Fabrication of copper nanoparticle composite nanogel for high‐efficiency management of Pseudomonas syringae pv. tabaci on tobacco

Abstract

BACKGROUND Copper nanoparticles (CuNPs) can release copper ions (Cu2+) to control bacterial diseases on crops. However, the high concentration of the CuNPs applied in disease controlling can highly limit their application. In this work, by in situ reducing CuNPs in alginate nanogels and coated with cetyl trimethyl ammonium chloride (CTAC), a CuNP composite nanogel was fabricated as a new nanopesticide with low copper content. RESULTS Data showed that the CTAC coating would affect the antibacterial activity and leaf surface adhesion of the nanogel, while CuNP content could also influence the membrane damage ability of the gel. The nanogel could depress the growth of bacteria by rupturing its membrane and show a minimum inhibitory concentration (MIC) as low as 500 μg mL−1, which only contain 58 μg mL−1 CuNP, and achieve a 64% of therapeutic efficiency (with 1000 μg mL−1 nanogel) in in vivo experiments, higher than that of commercial bactericide thiodiazole copper. Furthermore, the application of the nanogel can also perform a growth‐promoting effect on the plant, which may be due to the supplement of copper element provided by CuNP. CONCLUSION The CuNP composite nanogel fabricated in this work performed high leaf disease controllability and safety compared to the commercial bactericide thiodiazole copper. We hope this nanogel can provide a potential high‐efficiency nano‐bactericide that can be used in the leaf bacterial disease control.

Nanobiopesticides in sustainable agriculture: developments, challenges, and perspectives

“…151 The fabricated CuNP composite nanogel showed high controllability of Pseudomonas syringae on tobacco and safety compared to the commercial bactericide thiodiazole copper. 152 All these studies indicated that nanotechnology can improve the antibacterial activity of traditional biopesticides.…”

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“…50 This pathogen is responsible for bacterial wildfire, a devastating disease that can lead to substantial losses in plant production. 51 P. syringae pv. Tabaci infiltrates plants through natural openings such as stomata or wounds and can spread easily through water, wind, or contaminated tools.…”

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