Fabrication of colloidal silver-peptide nanocomposites for bacterial wound healing

Amyloid-Inspired Engineered Multidomain Amphiphilic Injectable Peptide Hydrogel─An Excellent Antibacterial, Angiogenic, and Biocompatible Wound Healing Material

“…From the CAC plot shown in Figure S12 , it appears to us that among all, LVK-PA possesses the smallest value of CAC (63 μM), which is quite expected from the presence of both “FF” and “WW” residues within its core structure. The π–π interaction among the “FF” and “WW” residues expedites the self-assembly process. ,, In this context, it is important to note that the presence of “FF” moiety on the “LVFFA” domain plays a crucial role in its aggregation property. − Both AIK-PA (97.7 μM) and VGK-PA (100.57 μM) show comparatively higher CAC and a lower rate of self-assembly than LVK-PA at a given concentration; again, the lesser hydrophobicity of the core amphiphile skeleton could be the reason. Solvatochromic dye Nile red being extremely sensitive toward the polar environment shows higher fluorescence upon its entrapment within self-assembled nanostructures containing a hydrophobic core.…”

“…The successful genesis of an engineered AMP-based biomaterial requires a fine balance between amphiphilic property in combination with its cationic nature and ability to acquire secondary structures so that it must not exert cytotoxicity to the mammalian cells. , Earlier Reches and Gazit identified “FF” dipeptide from the core hydrophobic region of amyloid beta 42 which established a broad application avenue of bioinspired short peptide-based material with multifaceted use. , Extrapolating its use Schnaider et al reported the use of “FF” dipeptide as the most minimalistic model of self-assembling peptide-based antimicrobial nanostructures . Following this, Ovais et al in their work used “FFssFF” as a capping agent for the formation of colloidal Ag/FFssFF composite nanoparticles having significant activity against both Gram-positive and Gram-negative bacterial strains . Shreds of evidence suggest that various hydrophobic beta aggregation-prone peptides similar to “FF” in combination with metal ions like Ag + , and Zn 2+ assembly can give rise to nanomaterials with improved antimicrobial and anti-biofilm effectivity. , However, the emergence of multidrug resistance bacteria, unavailability of biochemical cues, compromised polarity, and biocompatibility restricted their use as advanced tropical antibacterial wound healing therapeutics.…”

“…19 Following this, Ovais et al in their work used "FFssFF" as a capping agent for the formation of colloidal Ag/FFssFF composite nanoparticles having significant activity against both Gram-positive and Gramnegative bacterial strains. 20 Shreds of evidence suggest that various hydrophobic beta aggregation-prone peptides similar to "FF" in combination with metal ions like Ag + , and Zn 2+ assembly can give rise to nanomaterials with improved antimicrobial and anti-biofilm effectivity. 21,22 However, the emergence of multidrug resistance bacteria, unavailability of biochemical cues, compromised polarity, and biocompatibility restricted their use as advanced tropical antibacterial wound healing therapeutics.…”

Intestinal Toxicity of Metal Nanoparticles: Silver Nanoparticles Disorder the Intestinal Immune Microenvironment

“…Herein, we observed inflammatory reactions and intestinal microbiota alterations after long-term sublethal exposure to Ag NPs. Based on the physicochemical properties, the strong biological effects of Ag NPs would be mainly because of their small size and excellent colloidal stability (referring to the capping) . In addition, Ag NPs could be uptaken by DCs and induced abnormal maturation through ROS production.…”

“…Based on the physicochemical properties, the strong biological effects of Ag NPs would be mainly because of their small size and excellent colloidal stability (referring to the capping). 65 In addition, Ag NPs could be uptaken by DCs and induced abnormal maturation through ROS production. Instead of inducing cell death of DCs, the abnormal maturation of DCs activated the Bim signaling pathway and led to apoptosis.…”