Fabrication of chitosan nanoparticles with phosphatidylcholine for improved sustain release, basolateral secretion, and transport of lutein in Caco-2 cells

Shwetha, Hulikere Jagdish; Shilpa, Shivaprasad; Mukherjee, Mousumi Beto; Ambedkar, Rudrappa; Raichur, Ashok M.; Lakshminarayana, Rangaswamy

doi:10.1016/j.ijbiomac.2020.09.040

Cited by 16 publications

(17 citation statements)

References 45 publications

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“…This behaviour was not observed in the spectra of the ZH and GZH carriers loaded with lutein, and there was no significant difference between ZH and GZH carriers before and after lutein was loaded. The characteristic absorption peak of lutein weakened or disappeared, indicating that there was no chemical reaction between lutein and the carrier after emulsification but only physical interaction, and the infrared spectrum results also confirmed that lutein was successfully loaded onto it ( Shwetha et al, 2020 ).…”

Section: Resultsmentioning

confidence: 74%

Glycosylation of Zein Hydrolysate as a Nanocarrier for Lutein Delivery: Preparation and Stability

Jiao

Chang

et al. 2022

Front. Pharmacol.

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Lutein is a functional carotenoid that has a wide range of physiological benefits in humans. However, it easily degrades and becomes inactivated during storage and processing, resulting in low bioavailability. The development of new nanocarriers can effectively improve the stability and biological activity of lutein. In this study, zein hydrolysate (ZH) carriers were glycosylated with glucosamine (GLU) under the action of transglutaminase, and lutein-loaded glycosylated ZH nanoparticles (GZH-LUT) were constructed by liquid–liquid dispersion. The results showed that the GZH-LUT particles had a narrow size distribution in the range of 200–300 nm and a decreased zeta potential and polydispersity index. In particular, GZH trapped lutein more efficiently than ZH. In addition, GZH-LUT had better physical and chemical properties, including better water solubility, oxidative stability, and environmental stability than free lutein and ZH-LUT. These results indicate that glycosylated zein hydrolysate has the potential to be used as a novel protein-based nanocarrier to enhance the solubility and stability of lutein, which can further improve its bioavailability.

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Section: Resultsmentioning

confidence: 74%

Glycosylation of Zein Hydrolysate as a Nanocarrier for Lutein Delivery: Preparation and Stability

Jiao

Chang

et al. 2022

Front. Pharmacol.

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“…These carotenoidencapsulated zein-alginate NPs were further coated with PC by the conventional thin-film hydration method with slight modification. 30 Briefly, PC (0.5%) was dissolved in chloroform and dried using a Labconco CentriVap benchtop vacuum concentrator (Kansas City, MO); then, 1 mL of carotenoid-encapsulated zein-alginate suspension was added and hydrated with the lipid film in a water bath at 55 °C. This carotenoid zein-alginate-PC nanoemulsion was ultrasonicated at and kept at 4 °C until further analysis, bioaccessibility, and cell absorption studies.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, the surfactant action of lipids insists on disaggregation and homogeneous suspension to yield better carotenoid-encapsulated nanoemulsion or NPs. 19,30 Recently, a few reports concluded that bioaccessibility and bioavailability of carotenoids are higher with increased lipid content of excipient nanoemulsion. 31 The addition of lipids affects solubility, stability, and mobility of encapsulated nutrients including carotenoids; therefore, fabrication of NPs with dietary lipids is appropriate to improve absorption of vital nutrients or therapeutics by specific interaction in the GI environment.…”

Section: Introductionmentioning

confidence: 99%

Zein-Alginate-Phosphatidylcholine Nanocomplex Efficiently Delivers Lycopene and Lutein over Dietary-Derived Carotenoid Mixed Micelles in Caco-2 Cells

Shwetha

Arathi

Mukherjee

et al. 2022

J. Agric. Food Chem.

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This study evaluated the potency of zein-alginate-phosphatidylcholine nanoparticles (NPs) on bioaccessibility/intestinal uptake of encapsulated lycopene (LY) and lutein (LT) versus dietary absorption using simulated digestion and human intestinal Caco-2 cells. LY-zein-alginate-PC (LYZAP) and LT-zein-alginate-PC (LTZAP) NPs yield desired properties, which exhibit sustained release and are suitable for oral administration. Interestingly, co-treatment of LYZAP + LTZAP showed better release of carotenoids instead of individual treatment at intestinal pH. Bioaccessibility, cellular uptake, and basolateral secretion of LY and LT from NPs were significantly enhanced than micellar carotenoids (dietary mode of absorption). The increased absorption of carotenoids from NPs correlated with triglyceride levels. The intestinal cell uptake of carotenoids by nanoencapsulation may be due to endocytosis, paracellular, and SRB-1 protein-mediated transport. Overall, LYZAP and LTZAP NPs possess superior properties to control the release and cellular uptake of unique or distinct carotenoids. The inclusion of alginate and phosphatidylcholine in zein-based nanoencapsulation could be a promising strategy to improve carotenoid bioavailability.

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“…Furthermore, the in vivo oral pharmacokinetic study using Wistar rats revealed that the LTN-CHNPs exhibited 128.3% improved oral bioavailability compared to free LTN. In another study, Shwetha et al developed LTN-loaded CS and phosphatidylcholine-based NPs (LTN-CS-PPT-NPs) for improved oral delivery [214]. The LTN-CS-PPT-NPs revealed excellent drug release profiles in both SGF and SIF for up to 42 h of study.…”

Section: Luteinmentioning

confidence: 99%

Recent Advancement in Chitosan-Based Nanoparticles for Improved Oral Bioavailability and Bioactivity of Phytochemicals: Challenges and Perspectives

et al. 2021

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The excellent therapeutic potential of a variety of phytochemicals in different diseases has been proven by extensive studies throughout history. However, most phytochemicals are characterized by a high molecular weight, poor aqueous solubility, limited gastrointestinal permeability, extensive pre-systemic metabolism, and poor stability in the harsh gastrointestinal milieu. Therefore, loading of these phytochemicals in biodegradable and biocompatible nanoparticles (NPs) might be an effective approach to improve their bioactivity. Different nanocarrier systems have been developed in recent decades to deliver phytochemicals. Among them, NPs based on chitosan (CS) (CS-NPs), a mucoadhesive, non-toxic, and biodegradable polysaccharide, are considered the best nanoplatform for the oral delivery of phytochemicals. This review highlights the oral delivery of natural products, i.e., phytochemicals, encapsulated in NPs prepared from a natural polymer, i.e., CS, for improved bioavailability and bioactivity. The unique properties of CS for oral delivery such as its mucoadhesiveness, non-toxicity, excellent stability in the harsh environment of the GIT, good solubility in slightly acidic and alkaline conditions, and ability to enhance intestinal permeability are discussed first, and then the outcomes of various phytochemical-loaded CS-NPs after oral administration are discussed in detail. Furthermore, different challenges associated with the oral delivery of phytochemicals with CS-NPs and future directions are also discussed.

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Fabrication of chitosan nanoparticles with phosphatidylcholine for improved sustain release, basolateral secretion, and transport of lutein in Caco-2 cells

Cited by 16 publications

References 45 publications

Glycosylation of Zein Hydrolysate as a Nanocarrier for Lutein Delivery: Preparation and Stability

Glycosylation of Zein Hydrolysate as a Nanocarrier for Lutein Delivery: Preparation and Stability

Zein-Alginate-Phosphatidylcholine Nanocomplex Efficiently Delivers Lycopene and Lutein over Dietary-Derived Carotenoid Mixed Micelles in Caco-2 Cells

Recent Advancement in Chitosan-Based Nanoparticles for Improved Oral Bioavailability and Bioactivity of Phytochemicals: Challenges and Perspectives

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