Fabrication of calcium phosphate composite polymer/SLS-stabilized emulsion-based bioactive gels and their application for dentine tubule occlusion

Waiyawat, Jutharat; Kongsema, Mesayamas; Sinthuvanich, Chomdao; Chienthavorn, Orapin; Teanchai, Chayada; Akkarachaneeyakorn, Khrongkhwan

doi:10.1016/j.job.2019.11.002

Cited by 4 publications

(5 citation statements)

References 28 publications

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“…At present, the most advantageous bone graft material was the artificial bone compared to autogenous and allogeneic bone grafts. Calcium phosphate–based composites were the focus of research in the department of orthopedics, stomatology, and plastic surgery in recent years, which could achieve the same effect of autologous bone transplantation with the advantages of economic, wide source, and no immunogenicity ( Richter et al, 2019 ; Birkholz et al, 2016 ; Waiyawat et al, 2020 ; Ma et al, 2019 ). HE and Masson staining showed that the tibia was replaced by new bone tissue induced by the CTC composite.…”

Section: Discussionmentioning

confidence: 99%

Identification of Small-Molecule Inhibitors for Osteosarcoma Targeted Therapy: Synchronizing In Silico, In Vitro, and In Vivo Analyses

Liu

et al. 2022

Front. Bioeng. Biotechnol.

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Objective: The study aimed to explore a new approach for the treatment of osteosarcoma through combining biomaterials with next-generation small molecule–based targeted therapy.Methods: The model of osteosarcoma was established by 4-hydroxyaminoquinoline 1-oxide (4-HAQO) in mice while the collagen-thermosensitive hydrogel–calcium phosphate (CTC) biocomposites were prepared, and the small molecule inhibitors were virtually screened and synthesized. Then, for the osteosarcoma cell line, MG-63 cells were used to validate our bioinformatic findings in vitro, and the mouse osteosarcoma models were treated by combing CTC composites and small-molecule inhibitors after debridement.Results: Five compounds, namely, ZINC150338698, ZINC14768621, ZINC4217203, ZINC169291448, and ZINC85537017, were found in the ZINK database. Finally, ZINC150338698 was selected for chemical synthesis and experimental verification. The results of the MTT assay and Hoechst staining showed that the small-molecule inhibitor ZINC150338698 could significantly induce MG-63 cell death. Furthermore, CTC composites and ZINC150338698 could repair the bone defects well after the debridement of osteosarcoma. In addition, the biomaterials and small-molecule inhibitors have good biocompatibility and biosafety.Conclusion: Our findings not only offer systems biology approach-based drug target identification but also provide new clues for developing novel treatment methods for future osteosarcoma research.

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Section: Discussionmentioning

confidence: 99%

Identification of Small-Molecule Inhibitors for Osteosarcoma Targeted Therapy: Synchronizing In Silico, In Vitro, and In Vivo Analyses

Liu

et al. 2022

Front. Bioeng. Biotechnol.

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“…27 The properties of amorphous calcium phosphate can provoke the dissolution of calcium and phosphate ion and penetrate dentine tubules to facilitate the formation of amorphous calcium phosphate through the reprecipitation process. 15 Finally, to demonstrate the stability of occluding materials, the occluded dentine tubules was tested with acid soft drink. In SEM micrograph (Figure 6), the calcium phosphate particles in tubules were slightly dissolved and transformed to dentine layer.…”

Section: Discussionmentioning

confidence: 99%

“…Calcium phosphate nanoparticles were synthesized with an emulsion process modified from previous study. 15 Specifically, emulsions were prepared by mixing 2 mL of oleic acid and 0.055 g of sodium dodecyl sulphate (SDS) while stirring for 48 h at room temperature to produce a homogeneous solution. 100 µL of a calcium chloride aqueous solution (7.45 × 10 -4 moles, 7.45 M) was added into the above solution and stirred for 30 min to create water in an oil emulsion system.…”

Section: Preparation Of Calcium Phosphate Nanoparticles and Compositementioning

confidence: 99%

“…13 In this study, the emulsion technique is chosen to study the preparation of calcium phosphate containing bioactive gel due to its capacity to control particle sizes and morphology as it is a low-cost procedure and performed under mild conditions. 14,15 Oleic acid is monounsaturated fatty acid used as a component in many foods. The fatty acid is also found at the total content between 0.12% and 0.25% of human and various animal species' teeth.…”

Section: Introductionmentioning

confidence: 99%

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Tooth desensitizing calcium phosphate composite gelatin-based gel

Waiyawat

Kanjana

Kongsema

et al. 2020

Journal of Bioactive and Compatible Polymers

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Dentine sensitivity is a dental problem common in individuals aged between 20 and 50 years. The most effective treatment method involves occluding the exposed dental tubules. This study focused on the synthesis of calcium phosphate nanoparticles in the form of gel to use as a proof of concept for home-treatment of sensitive teeth. In this study, calcium phosphate nanoparticles were prepared using emulsion method, in which oleic fatty acid was employed as an external phase, and sodium dodecyl sulphate (SDS) was used as a surfactant to form water-in-oil nanodroplets. Finally, in order to facilitate gel formation, the gelatin solution was introduced at the final step. The amount of gelatin varied from 5 to 15 percent by weight, which was found to have an effect on the gels’ properties and the size of calcium phosphate nanoparticles embedded in gel. Based on the characterization, the calcium phosphate nanoparticles were spherical in shape, though the size decreased as the amount of gelatin increased. The gel embedding smallest nanoparticle, that is, gel-15%G, was successfully proven to be non-toxic and able to fully occlude the dentine tubules only after overnight application. According to acid challenge, the occluded materials can resist to acid solution via redissolvation and reprecipitaion process.

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“…However, the difficulty of precisely obtaining the size of CaP crystals is a big problem using this method. The microemulsion method is highly effective for regulating the size of CaP crystals due to the uniform and narrow channels of the microreactors strictly restricting the nucleation and growth of crystals [ 154 , 155 ]. The surfactants in the microemulsion play a critical role in modulating the CaP crystal size.…”

Section: Effects Of Sizes and Structural Characteristics Of Cap Mater...mentioning

confidence: 99%

Calcium Phosphate-Based Biomaterials for Bone Repair

Hou

Zhang

Zhou

et al. 2022

JFB

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Traumatic, tumoral, and infectious bone defects are common in clinics, and create a big burden on patient’s families and society. Calcium phosphate (CaP)-based biomaterials have superior properties and have been widely used for bone defect repair, due to their similarities to the inorganic components of human bones. The biological performance of CaPs, as a determining factor for their applications, are dependent on their physicochemical properties. Hydroxyapatite (HAP) as the most thermally stable crystalline phase of CaP is mostly used in the form of ceramics or composites scaffolds with polymers. Nanostructured CaPs with large surface areas are suitable for drug/gene delivery systems. Additionally, CaP scaffolds with hierarchical nano-/microstructures have demonstrated excellent ability in promoting bone regeneration. This review focuses on the relationships and interactions between the physicochemical/biological properties of CaP biomaterials and their species, sizes, and morphologies in bone regeneration, including synthesis strategies, structure control, biological behavior, and the mechanisms of CaP in promoting osteogenesis. This review will be helpful for scientists and engineers to further understand CaP-based biomaterials (CaPs), and be useful in developing new high-performance biomaterials for bone repair.

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Fabrication of calcium phosphate composite polymer/SLS-stabilized emulsion-based bioactive gels and their application for dentine tubule occlusion

Cited by 4 publications

References 28 publications

Identification of Small-Molecule Inhibitors for Osteosarcoma Targeted Therapy: Synchronizing In Silico, In Vitro, and In Vivo Analyses

Identification of Small-Molecule Inhibitors for Osteosarcoma Targeted Therapy: Synchronizing In Silico, In Vitro, and In Vivo Analyses

Tooth desensitizing calcium phosphate composite gelatin-based gel

Calcium Phosphate-Based Biomaterials for Bone Repair

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