Fabrication of Biomimetic Scaffolds with Oriented Porous Morphology for Cardiac Tissue Engineering

Zhang, Ting; Jin, Le; Fang, Yajun; Feng, Lin; Sun, Wenhuan; Xiong, Zhuo

doi:10.1166/jbt.2014.1255

Cited by 12 publications

(25 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From a structural perspective, an ideal scaffold should possess interconnected macro pores with sizes from 10 to 100 μm to facilitate cell infiltration and tissue formation, as well as similar topography of natural ECM that would have an impact on cell behavior such as cell adhesion and gene expression (Han et al, 2012;McMurtrey, 2014;Sakai et al, 2008;Sun et al, 2016;Zhang et al, 2014). For that purpose, here we developed a fabrication process to incorporate electrospun short fibers within biopolymer scaffold for tissue engineering applications.…”

Section: Discussionmentioning

confidence: 99%

“…Type A gelatin from porcine skin (purchased from Sigma Aldrich) was dissolved at 1 wt% concentration in deionized water at 60 C, this concentration was selected according to optimized value in (Zhang et al, 2014). The PCL short fibers were weighted and evenly dispersed in deionized water at 1 wt% concentration.…”

Section: Fiber Incorporated Scaffold Fabricationmentioning

confidence: 99%

“…The freeze-drying method is widely used to fabricate 3D porous scaffold for tissue engineering (Zhang et al, 2014). However, most freeze-dried scaffolds are composed of pores that are made from randomly or ordered distributed thin walls that lack the fibrous topography of the natural ECM (Ran et al, 2016;Sun, Li, Jiang, Sun, & Li, 2016).…”

mentioning

confidence: 99%

“…However, most freeze-dried scaffolds are composed of pores that are made from randomly or ordered distributed thin walls that lack the fibrous topography of the natural ECM (Ran et al, 2016;Sun, Li, Jiang, Sun, & Li, 2016). The mechanical properties of freeze-dried scaffold can be tailored by varying the thermal gradient during processing as described previously (Zhang et al, 2014), which would inevitably alter the pore size. The elastic modulus of freeze-dried scaffolds usually increases as pore size decreases, as previously reported in literature (Zhang et al, 2014).…”

mentioning

confidence: 99%

“…The mechanical properties of freeze-dried scaffold can be tailored by varying the thermal gradient during processing as described previously (Zhang et al, 2014), which would inevitably alter the pore size. The elastic modulus of freeze-dried scaffolds usually increases as pore size decreases, as previously reported in literature (Zhang et al, 2014). However, taking into account the variability of the tissue target of the regeneration, an average suitable pore size of scaffolds for tissue engineering applications could be considered in the range between 50 and 150 μm for efficient cell seeding, which suggests that the mechanical properties of scaffold increase, compromising the efficiency of cell seeding.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Novel biomimetic fiber incorporated scaffolds for tissue engineering

Fang

Liverani

Boccaccını

et al. 2019

J Biomedical Materials Res

Self Cite

View full text Add to dashboard Cite

From a structural perspective, an ideal scaffold ought to possess interconnected macro pores with sizes from 10 to 100 μm to facilitate cell infiltration and tissue formation, as well as similar topography to the natural extracellular matrix (ECM) which would have an impact on cell behavior such as cell adhesion and gene expression. For that purpose, here we developed a fabrication process to incorporate electrospun short fibers within freeze‐dried scaffolds for tissue engineering applications. Briefly, PCL short fibers were first produced from electrospun fibers with ultrasonication method. They were then evenly dispersed in gelatin solution and freeze‐dried to obtain fiber incorporated scaffolds. The resulting scaffolds exhibited hierarchical structure including major pores with sizes ranging from 50 to 150 μm and the short fibers dispersed in the thin walls of major pores, mimicking the fibrous feature of natural ECM. The short fibers were proven to modify the mechanical properties of scaffold and to facilitate cell adhesion and proliferation on the scaffold. As a promising scaffold for tissue engineering and regenerative medicine, the fiber incorporated scaffold may have further biomedical applications, in which the short fibers can act as drug release vehicles for growth factors or other biomolecules to promote vascularization.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Fiber Incorporated Scaffold Fabricationmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Novel biomimetic fiber incorporated scaffolds for tissue engineering

Fang

Liverani

Boccaccını

et al. 2019

J Biomedical Materials Res

Self Cite

View full text Add to dashboard Cite

show abstract

Optimizing Bifurcated Channels within an Anisotropic Scaffold for Engineering Vascularized Oriented Tissues

Fang

Ouyang

Zhang

et al. 2020

Adv Healthcare Materials

Self Cite

View full text Add to dashboard Cite

Despite progress in engineering both vascularized tissues and oriented tissues, the fabrication of 3D vascularized oriented tissues remains a challenge due to an inability to successfully integrate vascular and anisotropic structures that can support mass transfer and guide cell alignment, respectively. More importantly, there is a lack of an effective approach to guiding the scaffold design bearing both structural features. Here, an approach is presented to optimize the bifurcated channels within an anisotropic scaffold based on oxygen transport simulation and biological experiments. The oxygen transport simulation is performed using the experimentally measured effective oxygen diffusion coefficient and hydraulic permeability of the anisotropic scaffolds, which are also seeded with muscle precursor cells and cultured in a custom‐made perfusion bioreactor. Symmetric bifurcation model is used as fractal unit to design the channel network based on biomimetic principles. The bifurcation level of channel network is further optimized based on the oxygen transport simulation, which is then validated by DNA quantification assay and pimonidazole immunostaining. This study provides a practical guide to optimizing bifurcated channels in anisotropic scaffolds for oriented tissue engineering.

show abstract

Cell-Free HA-MA/PLGA Scaffolds with Radially Oriented Pores for In Situ Inductive Regeneration of Full Thickness Cartilage Defects

Dai

Gao

et al. 2016

Macromol. Biosci.

View full text Add to dashboard Cite

A bioactive scaffold with desired microstructure is of great importance to induce infiltration of somatic and stem cells, and thereby to achieve the in situ inductive tissue regeneration. In this study, a scaffold with oriented pores in the radial direction is prepared by using methacrylated hyaluronic acid (HA-MA) via controlled directional cooling of a HA-MA solution, and followed with photo-crosslinking to stabilize the structure. Poly(lactide-co-glycolide) (PLGA) is further infiltrated to enhance the mechanical strength, resulting in a compressive modulus of 120 kPa. In vitro culture of bone marrow stem cells (BMSCs) reveals spontaneous cell aggregation inside this type of scaffold with a spherical morphology. In vivo transplantation of the cell-free scaffold in rabbit knees for 12 w regenerates simultaneously both cartilage and subchondral bone with a Wakitani score of 2.8. Moreover, the expression of inflammatory factor interleukin-1β (IL-1β) is down regulated, although tumor necrosis factor-α (TNF-α) is remarkably up regulated. With the anti-inflammatory, bioactive properties and good restoration of full thickness cartilage defect in vivo, the oriented macroporous HA-MA/PLGA hybrid scaffold has a great potential for the practical application in the in situ cartilage regeneration.

show abstract

Fabrication of Biomimetic Scaffolds with Oriented Porous Morphology for Cardiac Tissue Engineering

Cited by 12 publications

References 0 publications

Novel biomimetic fiber incorporated scaffolds for tissue engineering

Novel biomimetic fiber incorporated scaffolds for tissue engineering

Optimizing Bifurcated Channels within an Anisotropic Scaffold for Engineering Vascularized Oriented Tissues

Cell-Free HA-MA/PLGA Scaffolds with Radially Oriented Pores for In Situ Inductive Regeneration of Full Thickness Cartilage Defects

Contact Info

Product

Resources

About