Fabrication of biodegradable textile scaffold based on hydrophobized hyaluronic acid

Zapotocky, Vojtech; Pospisilova, Martina; Janouchová, Kateřina; Švadlák, Daniel; Batova, Jana; Sogorkova, Jana; Čepa, Martin; Běťák, Jiří; Štěpánková, Veronika; Šuláková, Romana; Kulhánek, Jaromír; Pitucha, Tomas; Vranová, Jana; Duffy, Garry P.; Velebný, Vladimı́r

doi:10.1016/j.ijbiomac.2016.10.076

Cited by 19 publications

(12 citation statements)

References 35 publications

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“…Swollen palmHA textiles are firm, smooth and easy to handle during in vitro experiments and possibly during implantation. Zapotocky at al . studied the physical characterization of this material and its degradation.…”

Section: Resultsmentioning

confidence: 99%

“…The process of fiber formation and its characterization was described in details elsewhere . The knitted scaffold preparation is briefly mentioned in supporting information and described in detail in . The strips of scaffold were placed in a physiological saline with different concentrations of FNC, FNG, LAM, COL IV, and MGEL (together with UV activator) for 30 min (37°C) to form scaffold coating (0.05; 0.01 mg/mL −1 of FNC, 0.05; 0.01; 0.005 mg/mL −1 of FNG, 0.05; 0.01; 0.005; 0.001 mg/mL −1 of LAM and 10, 5 and 1 mg/mL −1 of MGEL).…”

Section: Methodsmentioning

confidence: 99%

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Optimization of cell growth on palmitoyl‐hyaluronan knitted scaffolds developed for tissue engineering applications

Sogorkova

Zapotocky

Čepa

et al. 2018

J Biomedical Materials Res

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Polysaccharides meet several criteria for a suitable biomaterial for tissue engineering, which include biocompatibility and ability to support the delivery and growth of cells. Nevertheless, most of these polysaccharides, for example dextran, alginate, and glycosaminoglycans, are highly soluble in aqueous solutions. Hyaluronic acid hydrophobized by palmitic acid and processed to the form of wet-spun fibers and the warp-knitted textile scaffold is water non-soluble, but biodegradable material, which could be used for the tissue engineering purpose. However, its surface quality does not allow cell attachment. To enhance the biocompatibility the surface of palmitoyl-hyaluronan was roughened by freeze drying and treated by different cell adhesive proteins (fibronectin, fibrinogen, laminin, methacrylated gelatin and collagen IV). Except for collagen IV, these proteins covered the fibers uniformly for an extended period of time and supported the adhesion and cultivation of dermal fibroblasts and mesenchymal stem cells. Interestingly, adipose stem cells cultivated on the fibronectin-modified scaffold secreted increasing amount of HGF, SDF-1, and VEGF, three key growth factors involved in cardiac regeneration. These results suggested that palmitoyl-hyaluronan scaffold may be a promising material for various applications in tissue regeneration, including cardiac tissue repair. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1488-1499, 2018.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Optimization of cell growth on palmitoyl‐hyaluronan knitted scaffolds developed for tissue engineering applications

Sogorkova

Zapotocky

Čepa

et al. 2018

J Biomedical Materials Res

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“…As was shown in the experiments in vitro , the presence of the RGD motive in the HA‐PH hydrogel structure improved its cell affinity, but in a limited way. Therefore, for the cell encapsulation, the bioadhesive properties of the HA‐PH‐RGD hydrogel were further enhanced by the incorporation of fibrinogen which offers cellular adhesive domains and has been previously proved as a natural additive to enhance cell survival, growth and proliferation …”

Section: Discussionmentioning

confidence: 99%

“…Then, we evaluated the neuroregenerative potential of HA‐PH‐RGD hydrogel alone or in combination with human Wharton's jelly‐derived mesenchymal stem cells (hWJ‐MSCs) after its injection into the subacute spinal cord hemisection. To further promote hWJ‐MSCs adhesion, HA‐PH‐RGD was combined with fibrinogen (HA‐PH‐RGD/F), which contains integrin binding sites, such as RGD, and has been widely used as a promising additive to enhance cell survival, growth, and proliferation . Moreover, fibrinogen is abundant in a blood plasma and can be easily extracted from the donors for the autologous use.…”

Section: Introductionmentioning

confidence: 99%

“…To further promote hWJ-MSCs adhesion, HA-PH-RGD was combined with fibrinogen (HA-PH-RGD/F), which contains integrin binding sites, such as RGD, and has been widely used as a promising additive to enhance cell survival, growth, and proliferation. 33,34 Moreover, fibrinogen is abundant in a blood plasma and can be easily extracted from the donors for the autologous use. We used hWJ-MSCs, as these cells represent an easily accessible source, with high potential for clinical applications.…”

Section: Introductionmentioning

confidence: 99%

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Injectable hydroxyphenyl derivative of hyaluronic acid hydrogel modified with RGD as scaffold for spinal cord injury repair

Zaviskova

Tukmachev

Dubisova

et al. 2018

J Biomedical Materials Res

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Hydrogel scaffolds which bridge the lesion, together with stem cell therapy represent a promising approach for spinal cord injury (SCI) repair. In this study, a hydroxyphenyl derivative of hyaluronic acid (HA-PH) was modified with the integrin-binding peptide arginine-glycine-aspartic acid (RGD), and enzymatically crosslinked to obtain a soft injectable hydrogel. Moreover, addition of fibrinogen was used to enhance proliferation of human Wharton's jelly-derived mesenchymal stem cells (hWJ-MSCs) on HA-PH-RGD hydrogel. The neuroregenerative potential of HA-PH-RGD hydrogel was evaluated in vivo in acute and subacute models of SCI. Both HA-PH-RGD hydrogel injection and implantation into the acute spinal cord hemisection cavity resulted in the same axonal and blood vessel density in the lesion area after 2 and 8 weeks. HA-PH-RGD hydrogel alone or combined with fibrinogen (HA-PH-RGD/F) and seeded with hWJ-MSCs was then injected into subacute SCI and evaluated after 8 weeks using behavioural, histological and gene expression analysis. A subacute injection of both HA-PH-RGD and HA-PH-RGD/F hydrogels similarly promoted axonal ingrowth into the lesion and this effect was further enhanced when the HA-PH-RGD/F was combined with hWJ-MSCs. On the other hand, no effect was found on locomotor recovery or the blood vessel ingrowth and density of glial scar around the lesion. In conclusion, we have developed and characterized injectable HA-PH-RGD based hydrogel, which represents a suitable material for further combinatorial therapies in neural tissue engineering. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1129-1140, 2018.

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The Role of Hyaluronic Acid in Tissue Engineering

Collins

Zamboni

Serafin

et al. 2021

Polysaccharides of Microbial Origin

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Fabrication of biodegradable textile scaffold based on hydrophobized hyaluronic acid

Cited by 19 publications

References 35 publications

Optimization of cell growth on palmitoyl‐hyaluronan knitted scaffolds developed for tissue engineering applications

Optimization of cell growth on palmitoyl‐hyaluronan knitted scaffolds developed for tissue engineering applications

Injectable hydroxyphenyl derivative of hyaluronic acid hydrogel modified with RGD as scaffold for spinal cord injury repair

The Role of Hyaluronic Acid in Tissue Engineering

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