Fabrication of aptamer-guided siRNA loaded lipopolyplexes for gene silencing of notch 1 in MDA-mb-231 triple negative breast cancer cell line

Gharaibeh, Lobna; Alshaer, Walhan; Wehaibi, Suha; Buqain, Rula Al; Alqudah, Dana A.; Al-Kadash, Abdulfattah; Al-Azzawi, Hafsa; Awidi, Abdalla; Bustanji, Yasser

doi:10.1016/j.jddst.2021.102733

Cited by 17 publications

(11 citation statements)

References 55 publications

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“…We used appropriate assay kits of the QuantiFluor RNA system to evaluate the mRNA encapsulation efficiency of select representative lipids. [30,31] This evaluation was necessary because not only does pKa impact mRNA delivery efficiency, but the mRNA encapsulation capacity of different lipids also influences mRNA delivery. As depicted in Figure 2a, only lipid A-based LNPs had an encapsulation efficiency of more than 50%, while others were low, likely due to the low ratio of lipids to mRNA in our system.…”

Section: Resultsmentioning

confidence: 99%

Soybean Oil‐Derived Lipids for Efficient mRNA Delivery

Tang,

Yu,

Hsu

et al. 2023

Advanced Materials

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The rapid progress in the development of COVID‐19 mRNA vaccines during the initial year of the pandemic has highlighted the significance of lipid nanoparticles in therapeutic delivery. Various lipid types have been investigated for the effective delivery of mRNA, each with unique functions and versatile applications. These range from their use in cancer immunotherapy and gene editing to their role in developing vaccines against infectious diseases. Nonetheless, continued exploration of novel lipids and synthetic approaches is necessary to further advance the understanding and expand the techniques for optimizing mRNA delivery. In this work, new lipids derived from FDA‐approved soybean oil are facilely synthesized and these are employed for efficient mRNA delivery. EGFP and Fluc mRNA are used to evaluate the delivery efficacy of the lipid formulations both in vitro and in vivo. Furthermore, organ‐specific targeting capabilities are observed in certain formulations, and their outstanding performance is demonstrated in delivering Cre mRNA for gene editing. These results showcase the potential of soybean oil‐derived lipids in mRNA delivery, offering utility across a broad spectrum of bioapplications.

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Section: Resultsmentioning

confidence: 99%

Soybean Oil‐Derived Lipids for Efficient mRNA Delivery

Tang,

Yu,

Hsu

et al. 2023

Advanced Materials

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“…A few decades ago, a new technology was discovered to help administer drugs more safely and with minimal dosage; this was referred to as nanoparticle drug delivery ( Khdair et al ., 2016 ). Nanoparticles are nanomaterials that bind drugs with high affinity and aid in their targeted delivery and controlled release ( Patra et al ., 2018 ; Alshaer et al ., 2019 ; Gharaibeh et al ., 2021 ; Lafi et al ., 2021 ). Tumor-targeted nanoparticles were used to improve the therapeutic index of different BH3 mimetics.…”

Section: Apoptotic Pathwaysmentioning

confidence: 99%

Unlocking the Therapeutic Potential of BCL-2 Associated Protein Family: Exploring BCL-2 Inhibitors in Cancer Therapy

Dakkak,

Taneera,

El-Huneidi

et al. 2024

Biomol Ther (Seoul)

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Apoptosis, programmed cell death pathway, is a vital physiological mechanism that ensures cellular homeostasis and overall cellular well-being. In the context of cancer, where evasion of apoptosis is a hallmark, the overexpression of anti-apoptotic proteins like Bcl2, Bcl-xL and Mcl-1 has been documented. Consequently, these proteins have emerged as promising targets for therapeutic interventions. The BCL-2 protein family is central to apoptosis and plays a significant importance in determining cellular fate serving as a critical determinant in this biological process. This review offers a comprehensive exploration of the BCL-2 protein family, emphasizing its dual nature. Specifically, certain members of this family promote cell survival (known as anti-apoptotic proteins), while others are involved in facilitating cell death (referred to as pro-apoptotic and BH3-only proteins). The potential of directly targeting these proteins is examined, particularly due to their involvement in conferring resistance to traditional cancer therapies. The effectiveness of such targeting strategies is also discussed, considering the tumor's propensity for anti-apoptotic pathways. Furthermore, the review highlights emerging research on combination therapies, where BCL-2 inhibitors are used synergistically with other treatments to enhance therapeutic outcomes. By understanding and manipulating the BCL-2 family and its associated pathways, we open doors to innovative and more effective cancer treatments, offering hope for resistant and aggressive cases.

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“… The publication of this work enlightened researchers toward further improvements in the targeted delivery of siRNA using nanoassemblies and DNA nanotechnology. In combination with other nanodelivery systems, such as lipid nanoparticles (LNPs) and exosomes, aptamers can significantly improve the efficiency of drug delivery by “coordinating” with other delivery systems. − The design of aptamer-functionalized LNPs is shown in Figure a. Liang et al used cell-SELEX to select aptamer CH6 that specifically targets both rat and human osteoblasts.…”

Section: Aptamer-based Sirna Targeted Deliverymentioning

confidence: 99%

Aptamer-Based Targeted Delivery of Functional Nucleic Acids

Xie

Sun

et al. 2023

J. Am. Chem. Soc.

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Functional nucleic acid (NA)-based drugs have a broad range of applications since they allow the alteration and control of gene/protein expression patterns in cells. In principle, functional NAs need to be transported precisely and efficiently to target cells to guarantee both functionality and safety. Owing to their negative charges, it is difficult for natural NAs to cross the cell membrane composed of lipid bilayer and enter targeted cells. Worse still, the delivery of undirected functional NAs to nontargeted healthy cells and/or tissues would induce unpredictable adverse effects. Therefore, the precisely targeted delivery of functional NAs to specific cells/organs, particularly in extrahepatic sites, is required. Since aptamers can bind to various proteins on the cell surface with high specificity and selectivity, they can serve as the molecular recognition units to accurately bind target cells and subsequently enable the efficient delivery of cargo. In this perspective, we summarize the original, proof-of-concept aptamer-based strategies for the targeted delivery of functional NAs. A few specific examples are then discussed, followed by our perspectives on some of the challenges and opportunities that lie ahead.

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Fabrication of aptamer-guided siRNA loaded lipopolyplexes for gene silencing of notch 1 in MDA-mb-231 triple negative breast cancer cell line

Cited by 17 publications

References 55 publications

Soybean Oil‐Derived Lipids for Efficient mRNA Delivery

Soybean Oil‐Derived Lipids for Efficient mRNA Delivery

Unlocking the Therapeutic Potential of BCL-2 Associated Protein Family: Exploring BCL-2 Inhibitors in Cancer Therapy

Aptamer-Based Targeted Delivery of Functional Nucleic Acids

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