Fabrication of antibody-loaded microgels using microfluidics and thiol-ene photoclick chemistry

Gregoritza, Kathrin; Abstiens, Kathrin; Graf, Moritz; Goepferich, Achim

doi:10.1016/j.ejpb.2018.02.024

Cited by 21 publications

(22 citation statements)

References 50 publications

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“…However, microgels possess unique properties of both hydrogels and colloidal particles, such as structural integrity, compartmentalization, orthogonal functionalization, softness, deformability, permeability, stimuli-responsiveness, reversible swelling, and adaptivity, and have been found to be promising for a wide range of applications in various fields including controlled drug release, separation technology, bio-, and chemical sensors, etc. [312][313][314][315][316][317][318][319][320][321][322][323][324][325][326] Microgels can be fabricated mainly via emulsion polymerization with an added surfactant, surfactant-free emulsion polymerization (SFEP), inverse mini-, and microemulsion polymerization, as well as the crosslinking of prepolymer chains, etc. [327,328] Typically, the techniques used to break-up and gel the particles for microgel formation will determine the final microgel properties, including the structure, size, and strength [329] and the presence and amount of crosslinks determine the "colloidal" or "macromolecular" character of the microgel.…”

Section: Microgelsmentioning

confidence: 99%

Hydrogel: Diversity of Structures and Applications in Food Science

Jia

Luo

2021

Food Reviews International

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Hydrogels are a series of soft and wet materials with three dimensions of crosslinked networks. Hydrogels have attracted great attention due to their diverse functional properties, and their wide range of applications, such as in soft robots and actuators, stretched electronic devices, tissue engineering materials, controlled-release drug delivery vehicles, biomedicine materials, food science, and (bio)sensors. In general, there are four core concerns in hydrogel science, including the polymer source, structure fabrication, gel function, and gel applications. According to the logic that the "structure determines function", it is believed that rational design of structures can effectively regulate the functions and applications of hydrogels. Hence, in the current review, "structure" as the core topic will be highly regarded, and the crosslinking mechanisms and structural diversity of hydrogels are comprehensively summarized. Additionally, hydrogels also show their great application potential in food science. Hence, the current review also pays more attention to the application of hydrogels in food nutrition and health, food engineering and processing, and food safety. It is whished that this review not only serves as a reference for improving the comprehensive understanding of the structural design of hydrogels but also provides a forward-looking idea for hydrogel applications in food science.

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Section: Microgelsmentioning

confidence: 99%

Hydrogel: Diversity of Structures and Applications in Food Science

Jia

Luo

2021

Food Reviews International

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“…Visible light photoinitiation is advantageous for the encapsulation of biological materials as UV radiation can cause DNA damage and accelerate tissue aging and cancer onset. Blue light photo-initiators that can be used are camphorquinone [ 82 ], eosin Y [ 83 ], and riboflavin [ 84 , 85 ].…”

Section: Microfluidic Production Of Spherical Matrix-type Microgelsmentioning

confidence: 99%

Crosslinking Strategies for the Microfluidic Production of Microgels

2021

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This article provides a systematic review of the crosslinking strategies used to produce microgel particles in microfluidic chips. Various ionic crosslinking methods for the gelation of charged polymers are discussed, including external gelation via crosslinkers dissolved or dispersed in the oil phase; internal gelation methods using crosslinkers added to the dispersed phase in their non-active forms, such as chelating agents, photo-acid generators, sparingly soluble or slowly hydrolyzing compounds, and methods involving competitive ligand exchange; rapid mixing of polymer and crosslinking streams; and merging polymer and crosslinker droplets. Covalent crosslinking methods using enzymatic oxidation of modified biopolymers, photo-polymerization of crosslinkable monomers or polymers, and thiol-ene “click” reactions are also discussed, as well as methods based on the sol−gel transitions of stimuli responsive polymers triggered by pH or temperature change. In addition to homogeneous microgel particles, the production of structurally heterogeneous particles such as composite hydrogel particles entrapping droplet interface bilayers, core−shell particles, organoids, and Janus particles are also discussed. Microfluidics offers the ability to precisely tune the chemical composition, size, shape, surface morphology, and internal structure of microgels by bringing multiple fluid streams in contact in a highly controlled fashion using versatile channel geometries and flow configurations, and allowing for controlled crosslinking.

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“…Visible light photoinitiation is advantageous for encapsulation of biological materials since UV radiation can cause DNA damage and accelerate tissue aging and cancer onset. Blue light photo-initiators that can be used are camphorquinone [77], eosin Y [78], and riboflavin [79,80].…”

Section: Photopolymerisationmentioning

confidence: 99%

“…Water soluble pre-polymers modified by introduction of cross-linkable molecules can be used instead of monomers. The examples of such modified polymers used for microfluidic production of microgels are dextran-hydroxyethyl methacrylate (dextran-HEMA) [85], gelatin-methacryloyl (GelMA) [86,87], poly(N-isopropylacrylamide-dimethylmaleimide), (P(NIPAAm-DMMI)) [88], poly(ethylene glycol diacrylate) (PEGDA) [89], poly(ethylene glycol methyl ether acrylate) (PEGMA) [90], poly(ethylene glycol) norbornene (PEG-NB) [78], and 6-armed acrylated PEG [91].…”

Section: Photopolymerisationmentioning

confidence: 99%

Crosslinking Strategies for Microfluidic Production of Microgels

Chen¹,

Bolognesi²,

Vladisavljević³

2021

Preprint

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This article provides a systematic review of the crosslinking strategies used to produce microgel particles in microfluidic chips. Various ionic crosslinking methods for gelation of charged pol-ymers are discussed, including external gelation via crosslinkers dissolved or dispersed in the oil phase, internal gelation methods using crosslinkers added to the dispersed phase in their non-active forms, such as chelating agents, photo-acid generators, sparingly soluble or slowly hydrolyzing compounds, and methods involving competitive ligand exchange, rapid mixing of polymer and crosslinking streams, and merging polymer and crosslinker droplets. Covalent crosslinking methods using enzymatic oxidation of modified biopolymers, photo-polymerization of crosslinkable monomers or polymers, and thiol-ene “click” reactions are also discussed, as well as the methods based on sol-gel transitions of stimuli responsive polymers triggered by pH or temperature change. In addition to homogeneous microgel particles, the production of structurally heterogeneous particles such as composite hydrogel particles entrapping droplet interface bi-layers, core-shell particles, organoids, and Janus particles are also discussed. Microfluidics offers the ability to precisely tune chemical composition, size, shape, surface morphology, and internal structure of microgels by bringing in contact multiple fluid streams in a highly controlled fashion using versatile channel geometries and flow configurations and allowing controlled crosslinking.

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Fabrication of antibody-loaded microgels using microfluidics and thiol-ene photoclick chemistry

Cited by 21 publications

References 50 publications

Hydrogel: Diversity of Structures and Applications in Food Science

Hydrogel: Diversity of Structures and Applications in Food Science

Crosslinking Strategies for the Microfluidic Production of Microgels

Crosslinking Strategies for Microfluidic Production of Microgels

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