Fabrication of an ultrasensitive and single-use graphite paper based immunosensor for Neuropeptide Y detection: A promising biosensing system for early detection of childhood obesity

Özcan, Burcu; Sezgintürk, Mustafa Kemal

doi:10.1016/j.mtcomm.2022.104797

Cited by 3 publications

(4 citation statements)

References 36 publications

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“…Both bioreceptors can then be immobilized on EDC/NHS-treated surfaces, as demonstrated by Chun et al (2013) and Nxele and Nyokong (2021) . Antibodies were immobilized on rGO treated with EDC/NHS to develop a novel graphite paper-based bioelectrode ( Özcan and Sezgintürk, 2022 ). The antigens can be immobilized in amine-functionalized rGO ( Patil et al, 2022 ) for antibody or aptamer testing.…”

Section: Antibody Aptamer and Nanomaterials Synergistic Systemsmentioning

confidence: 99%

“…Moreover, rGO also introduces nanoporous structures to the electrode’s surface, resulting in a high specific surface area that can accommodate more electroactive nanomaterials and deliver a higher electrochemical response. The application of rGO in developing ultrasensitive graphite paper-based immunosensors was reported ( Özcan and Sezgintürk, 2022 ).…”

Section: Antibody Aptamer and Nanomaterials Synergistic Systemsmentioning

confidence: 99%

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A review of antibody, aptamer, and nanomaterials synergistic systems for an amplified electrochemical signal

Reaño,

Escobar

2024

Front. Bioeng. Biotechnol.

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The synergy between biomolecules with inorganic nanomaterials and nanoparticles has been investigated over the past years, primarily to improve biomarker reception, generate signals, and amplify the signals generated. In this paper, several articles on aptamer-based and antibody-based electrochemical biosensors that target antigens were examined. Among the key characteristics identified were the electrochemical platform development, which includes the usage of nanomaterials as electroactive or electrocatalytic labels, crosslinking of the biological agent with inorganic compounds, and electrode coating to provide an electronic source and support efficient electron transfer. A single approach using labeled or unlabeled biological receptors has become advantageous due to its simple architecture and more straightforward application method. However, the dual system approach allows the incorporation of more nanomaterials to boost the signal and add more features to the electrochemical system. The dual system approach uses a capture and reporter probe in a competitive or sandwich detection format. The reporter probe is often labeled by an electroactive or electrocatalytic compound or immobilized in a nanocarrier, resulting in an increase in measured peak current in proportion to the target’s concentration. The reported limit of detection and linear range for each platform is presented to assess its efficiency. Generally, the dual system aptasensor showed higher sensitivity, stability, and reproducibility than the immunosensor in comparable settings. The aptasensor showed promising results for the development of point-of-care type applications.

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Section: Antibody Aptamer and Nanomaterials Synergistic Systemsmentioning

confidence: 99%

Section: Antibody Aptamer and Nanomaterials Synergistic Systemsmentioning

confidence: 99%

A review of antibody, aptamer, and nanomaterials synergistic systems for an amplified electrochemical signal

Reaño,

Escobar

2024

Front. Bioeng. Biotechnol.

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“…The efficiency of antibody binding with the target protein may depend on the amount of immobilized ligands on the surface of the electrodes [208,209]. It was noted in [78] that excess antibody molecules to the Aβ42 protein bound on the surface created a steric barrier for the protein molecules to approach the antibody molecules in the correct orientation.…”

Section: Impedimetric Detection Of Proteins Using Antibodiesmentioning

confidence: 99%

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Sitkov,

Ryabko,

Moshnikov

et al. 2024

Micromachines

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Impedimetric biosensors represent a powerful and promising tool for studying and monitoring biological processes associated with proteins and can contribute to the development of new approaches in the diagnosis and treatment of diseases. The basic principles, analytical methods, and applications of hybrid impedimetric biosensors for express protein detection in biological fluids are described. The advantages of this type of biosensors, such as simplicity and speed of operation, sensitivity and selectivity of analysis, cost-effectiveness, and an ability to be integrated into hybrid microfluidic systems, are demonstrated. Current challenges and development prospects in this area are analyzed. They include (a) the selection of materials for electrodes and formation of nanostructures on their surface; (b) the development of efficient methods for biorecognition elements’ deposition on the electrodes’ surface, providing the specificity and sensitivity of biosensing; (c) the reducing of nonspecific binding and interference, which could affect specificity; (d) adapting biosensors to real samples and conditions of operation; (e) expanding the range of detected proteins; and, finally, (f) the development of biosensor integration into large microanalytical system technologies. This review could be useful for researchers working in the field of impedimetric biosensors for protein detection, as well as for those interested in the application of this type of biosensor in biomedical diagnostics.

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“…Biosensors are advantageous and favored devices that are utilized to control target analyte materials in biological reactions and possess sensing capabilities [15]. Biosensors typically perform their functions as a result of a selective, rapid, and continuous reaction to a biologically or chemically active substance [16,17].…”

Section: Introductionmentioning

confidence: 99%

Tümör Nekrozis Faktörü-alfa biyobelirteç tayini için pratik ve tek kullanımlık ITO-PET bazlı immunosensör platformu

Özcan

2023

Hacettepe Journal of Biology and Chemistry

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This investigation displays a novel, practical indium tin oxide- polyethylene terephthalate (ITO-PET) based electrochemical biosensor for the Tumor Necrosis Factor-alpha (TNFα) biomarker determination. The ITO-PET electrode is a very advantageous preferred semiconductive electrode material. It has a lot of great features such as easy to prepare, cheapness, flexibility, stability. It also allows determining an analyte at very low concentrations and provides a great wide concentration range for analyte analysis. Electrochemical Impedance Spectroscopy (EIS) and Cyclic Voltammetry (CV) were used for the evaluation of biosensors, including the immobilization procedure, the investigation of the optimum conditions, and the characterization of biosensors. The immunosensor's electrode surface morphology during the immobilization process was observed using a Scanning Electron Microscope (SEM). In addition, Impedance measurement at a single frequency was used to characterize anti-TNFα and TNFα interactions (SFI). The clinical effectiveness of the developed biosensor was investigated by testing it with real human serum samples. Moreover, the fabricated immunosensor presents long shelf life, analysis of the antigen concentrations at picogram level (0.02 pg mL-1 -2.56 pg mL-1), reproducibility, reusability (11 times) and high sensitivity.

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Fabrication of an ultrasensitive and single-use graphite paper based immunosensor for Neuropeptide Y detection: A promising biosensing system for early detection of childhood obesity

Cited by 3 publications

References 36 publications

A review of antibody, aptamer, and nanomaterials synergistic systems for an amplified electrochemical signal

A review of antibody, aptamer, and nanomaterials synergistic systems for an amplified electrochemical signal

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Tümör Nekrozis Faktörü-alfa biyobelirteç tayini için pratik ve tek kullanımlık ITO-PET bazlı immunosensör platformu

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