Fabrication of an electrochemical biosensor based on Fe3O4 nanoparticles and uricase modified carbon paste electrode for uric acid determination

Erdoğan, Zehra Özden; Küçükkolbaşı, Semahat

doi:10.1007/s00706-021-02749-z

Cited by 13 publications

(7 citation statements)

References 33 publications

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“…Erdogan et al reported a uricase electrochemical biosensor based on ferric oxide nanoparticles (Fe 3 O 4 NPs) modification. [12] In this study, Fe 3 O 4 NPs were modified onto the CPE to enhance its conductivity. In addition, the strong adsorption force and high specific surface area of Fe 3 O 4 NPs were used to increase the loading capacity of the sensor for uricase.…”

Section: Application Of Metal and Its Derivative Nanomaterials In Enz...mentioning

confidence: 99%

“…[9] The fluorescence has a narrow detection range and poor anti-interference capabilities, the colorimetry requires high conditions, [6] the spectrophotometry has poor selectivity, [10] the HPLC involves complicated pretreatment processes, uses harmful solvents, [11] and requires expensive equipment. [12] The electrochemical method has attracted great attention in the field of biosensors due to the advantages of simple operation, low cost, and good selectivity. [13] Commonly used electrochemical methods for uric acid testing include cyclic voltammetry (CV), [14] linear sweep voltammetry (LSV), [15] electrochemical impedance spectroscopy (EIS), [16] chronocoulometry (CC), [17] electrochemiluminescence (ECL) and so on.…”

Section: Introductionmentioning

confidence: 99%

“…used electrochemical method to prepare a non‐enzymatic H 2 O 2 nanoprobe based on gold sputtering layered gold dendrite, and realized the detection of uric acid content in blood [9] . The fluorescence has a narrow detection range and poor anti‐interference capabilities, the colorimetry requires high conditions, [6] the spectrophotometry has poor selectivity, [10] the HPLC involves complicated pretreatment processes, uses harmful solvents, [11] and requires expensive equipment [12] . The electrochemical method has attracted great attention in the field of biosensors due to the advantages of simple operation, low cost, and good selectivity [13] .…”

Section: Introductionmentioning

confidence: 99%

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Enzymatic and Non‐Enzymatic Uric Acid Electrochemical Biosensors: A Review

et al. 2023

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In recent years, the development of electrochemical biosensors for uric acid has made great achievements. Firstly, uric acid electrochemical biosensors were classified according to their reaction mechanism. Then, the reaction mechanism of the uric acid sensor and the application of nano‐modified materials were deeply analyzed from the perspective of non‐enzyme and enzymes. In this paper, the catalytic oxidation capacity, enzyme adsorption effect, conductivity, robustness, detection range, and detection limit of uric acid sensors were discussed and compared. Finally, the advantages of acid‐sensitive electrochemical biosensors were summarized, and the constructive recommendations were proposed for improving the deficiencies of acid biosensors. The potential for further development in this area was also discussed.

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Section: Application Of Metal and Its Derivative Nanomaterials In Enz...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enzymatic and Non‐Enzymatic Uric Acid Electrochemical Biosensors: A Review

et al. 2023

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“…The surface stress-based membrane biosensor (MBios) has shown improvements over existing sensors in terms of price, detection speed, and operability. Hence, the MBios has received a lot of attention recently as a reliable technique for HSA detection. ,− …”

Section: Introductionmentioning

confidence: 99%

“…Hence, the MBios has received a lot of attention recently as a reliable technique for HSA detection. 9 , 12 − 15 …”

Section: Introductionmentioning

confidence: 99%

Multilayer Heterogeneous Membrane Biosensor Based on Multiphysical Field Coupling for Human Serum Albumin Detection

et al. 2023

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A factor closely associated with renal disease status in clinical diagnosis is abnormal human serum albumin (HSA) concentration levels in human body fluids urine, serum, etc. The surface stress biosensor was developed as a new type of biosensor to detect protein molecule concentration and has a wide range of clinical applications. However, further sensitivity improvement is required to achieve higher detection performance. Herein, MXene/PDMS/ Fe 3 O 4 /PDMS of the multilayer heterogeneous membrane biosensor (MHBios) based on the coupling of the magnetic field, electric field, and surface stress field was successfully developed to achieve high sensitivity HSA detection through magnetic sensitization. The modified antibody specifically binds to HSA at the AuNP layer, allowing the biosensor to convert the surface stress caused by PDMS film deformation into an electrical signal. When the biosensor was exposed to a uniform magnetic field, the conductive path of the conductive layer was reshaped further as the magnetic force amplified the deformation of the PDMS film, enhancing the conversion of biological signals to electrical signals. The results exhibited that the detection limit (LOD) of the MHBios was 78 ng/mL when HSA concentration was 0−50 μg/ mL, which was markedly lower than the minimum diagnostic limit of microalbuminuria. Furthermore, the MHBios detected HSA in actual samples, confirming the potential for early disease screening.

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