Fabrication of Amperometric Biosensing Systems Focusing on Attachment of High Substrate Selectivity

Abstract

The techniques for attaching high substrate selectivity to the amperometric biosensing systems are summarized with classification of membrane techniques, electron mediation techniques, electrodes, and electrochemical techniques.

Contributions by a Novel Edge Effect to the Permselectivity of an Electrosynthesized Polymer for Microbiosensor Applications

“…The design of biosensors for implantation in functioning biological tissues is an important area of research with significant socioeconomic impact. − Depending on the target analyte, different signal transduction pathways have been exploited in the design of enzyme-based biosensors, including direct oxidation of reduced oxidases, − dehydrogenase chemistries, − redox mediators, − and spectrophotometric approaches. − However, “first generation” electrochemical biosensors, based on reactions −, remain the most common design for many applications. ,,− The majority of these are oxidase-based devices designed to operate in amperometric mode, detecting H 2 O 2 generated by the reaction of the co-substrate (dioxygen) with the reduced form of the enzyme. Substrate + Ox/FAD → Products + Ox/FADH 2 Ox/FADH 2 + O 2 → Ox/FAD + H 2 O 2 H 2 O 2 → O 2 + 2H + + 2e − …”

“…The design of biosensors for implantation in functioning biological tissues is an important area of research with significant socioeconomic impact. [1][2][3][4] Depending on the target analyte, different signal transduction pathways have been exploited in the design of enzyme-based biosensors, including direct oxidation of reduced oxidases, [5][6][7] dehydrogenase chemistries, [8][9][10] redox mediators, [11][12][13] and spectrophotometric approaches. [14][15][16] However, "first generation" electrochemical biosensors, based on reactions 1-3, remain the most common design for many applications.…”

Elimination of Influence by Interference Substance on Amperometric Enzyme Biosensor Response Using Wavelet Transformation

“…(5) The current due to lactose is estimated by Eq. (11). It is noted that the current response of the bi-enzyme type lactose biosensor for lactose and ascorbic acid is independent, respectively.…”

“…In this case, if the cost of the membrane is expensive, the price of one biosensor tip may be very expensive. 8 Kuwabata et al [9][10][11] applied electrochemical impedance spectroscopy (EIS) to electrochemical glucose sensing without any selective permeable membranes. EIS is one of the nondestructive methods for measuring the current response to the application of sinusoidal AC voltage at various frequencies.…”

Effects of applied potential on the mass of non-conducting poly(ortho-phenylenediamine) electro-deposited on EQCM electrodes: comparison with biosensor selectivity parameters

“…From the first description of an enzyme-modified electrode to monitor concentration dynamics of a specific analyte, 1 through initial attempts at theoretical descriptions of the behaviour of these 'biosensors', 2,3 their application as analytical tools is continuing to grow and diversify in areas such as environmental surveillance, batch food analysis and clinical monitoring, and is beginning to impact on quality-oflife issues. [4][5][6][7][8] Depending on the target analyte, different signal transduction pathways have been exploited in the design of enzyme-based biosensors, including direct oxidation of reduced oxidases, [9][10][11] dehydrogenase chemistries, [12][13][14] redox mediators, [15][16][17] and spectrophotometric approaches. [18][19][20] However, 'first generation' electrochemical biosensors remain the most common strategy for many applications, [21][22][23][24][25] the majority being oxidase-based devices designed to operate in amperometric mode, detecting H 2 O 2 generated by the reaction of co-substrate (dioxygen) with the reduced form of the enzyme (reactions (1)-( 3)).…”