Fabrication of a Three-Layer PDMS Pneumatic Microfluidic Chip for Micro Liquid Sample Operation

Liu, Xuling; Li, Songjing

doi:10.1177/2472630319870126

Cited by 9 publications

(4 citation statements)

References 29 publications

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“…The research of Bong developed a pressure-driven control system that enables rapid and precise manipulation of complex microflows and further explored its utility in continuous-and pulsed-flow microfluidic procedures for the synthesis and analysis of microparticles [19] . Prior studies found that simulation analysis and experimental research on the performance of the on-chip pneumatic micro actuator of a pneumatic microfluidic chip, including the simulation analysis of the volume and pressure change process in the charging and exhaust process of the actuated chamber, and observed the liquid flow characteristics controlled by air pressure with a type of microscope and verified its working reliability [20][21][22] . Previous studies, both experiments and simulations about pneumatic micro actuators, have focused on influencing factors of air pressure fluctuation, air chamber geometries, or air pressure on the deformation of actuated membranes.…”

Section: Nomenclature E(t)mentioning

confidence: 98%

Research of control method for pneumatic control of pneumatic microchips

Liu¹,

Zuo²,

Yang³

et al. 2022

SLAS Technology

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Section: Nomenclature E(t)mentioning

confidence: 98%

Research of control method for pneumatic control of pneumatic microchips

Liu¹,

Zuo²,

Yang³

et al. 2022

SLAS Technology

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“…Additionally, PDMS demonstrates hydrophobic characteristics that can be used to regulate fluid flow inside the microfluidic device [72]. The hydrophobic properties of PDMS make it easier for well-defined channels and droplets to develop, providing fine-grained control over the transport of samples and reagents [73].…”

Section: (C)mentioning

confidence: 99%

Designing Microfluidic PCR Chip Device Using CFD Software for the Detection of Malaria

Austria,

Garcia,

Caparanga

et al. 2023

Preprint

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Polymerase chain reaction (PCR) technique is one of the molecular methods in amplifying DNA for the detection of malaria. However, the limitations of PCR especially when used for routine clinical practice can hamper its sensitivity and specificity. With that, this study focuses on designing a microfluidic device that will mimic the function of a conventional genus-specific PCR based on 18S rRNA gene to detect malaria parasites (Plasmodium falciparum) at low grade parasitemia. The design was drawn and simulated using ANSYS 14.5 Computational Fluid Dynamics (CFD). The simulation shows that adding loops to the design increases its relative deviation but to a minimal extent as compared to having only a straight path design, which indicates that looping is acceptable in designing a microfluidic device to minimize chip length. Also, increasing the cross-sectional area of the fluid path decreases the efficiency of the design, thus, the design with a relatively smaller cross-sectional area is favored. And lastly, among the three materials utilized, the chip made of polypropylene is the most efficient with a relative deviation of 0.94 as compared to polycarbonate and polydimethylsiloxane which have relative deviations of 2.78 and 1.92, respectively.

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“…2) of PAH-chip design and fabrication and cell seeding on the device so that any researchers without an engineering background can prepare the chips, grow PAH cells, study the PAH pathophysiology, and screen anti-PAH drugs. There are several published protocols on polydimethylsiloxane (PDMS)-based chips are available [13][14][15][16][17][18][19][20][21][22][23] , but our protocol is different in terms of application of the device and the depth of the details. Moreover, the published protocols lack details, but here we have added every detail in each step to prepare PDMS chips in studying the pathophysiology of PAH.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic device for recapitulating PAH-afflicted pulmonary artery: design, fabrication, and on-chip cell culture

Ahsan

2022

Preprint

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Pulmonary arterial hypertension (PAH), a rare condition and a hyperproliferative vascular disorder, is characterized by vascular remodeling of the intrapulmonary arterial wall, resulting in elevated pulmonary vascular resistance, right ventricular hypertrophy, and eventually right heart failure and death. Although animal models can reproduce the chief feature of the disease, i.e., elevated pulmonary arterial pressure, they do not accurately portray the biology of human PAH. Further, the FDA Modernization Act 2021 encourages using alternatives to animal models such as organ chip models in non-clinical studies because the latter models are not only humane but also expected to expedite drug development and discovery. As such, we recently established the feasibility of designing, developing, and deploying a PAH-on-a-chip for studying PAH pathophysiology and screening for anti-PAH drugs in our laboratory. This PAH-chip model mimics five layers of the pulmonary artery and allows the growing of adult PAH cells in separate channels, facilitating cell-cell and cell-matrix interactions. Importantly, the device can reconstruct the major clinical features of PAH, including arterial muscularization and plexiform lesions. Here, we described a step-by-step detailed method of PAH-chip design and fabrication and cell seeding on the device so that any biologist can prepare the device and study PAH pathophysiology in a laboratory setting. Our protocol is different from other published methods of PDMS-based chips in terms of application of the device and the depth of the details. We envision that this method of chip fabrication can also be used to study other pulmonary vascular disorders.

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Fabrication of a Three-Layer PDMS Pneumatic Microfluidic Chip for Micro Liquid Sample Operation

Cited by 9 publications

References 29 publications

Research of control method for pneumatic control of pneumatic microchips

Research of control method for pneumatic control of pneumatic microchips

Designing Microfluidic PCR Chip Device Using CFD Software for the Detection of Malaria

Microfluidic device for recapitulating PAH-afflicted pulmonary artery: design, fabrication, and on-chip cell culture

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