Fabrication of a three-dimensional β-tricalcium-phosphate/gelatin containing chitosan-based nanoparticles for sustained release of bone morphogenetic protein-2: Implication for bone tissue engineering

Bastami, Farshid; Paknejad, Zahrasadat; Jafari, Maissa; Salehi, Majid; Rad, Maryam Rezai; Khojasteh, Arash

doi:10.1016/j.msec.2016.10.084

Cited by 70 publications

(43 citation statements)

References 55 publications

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“…Many conventional strategies, including implantation of pre-fabricated constructs or designing delivery systems of growth factors, have been investigated with the aim of improving the healing process [55, 56]. However, there are still challenges in fabrication of an ideal scaffold with proper composition, physical structure and ability of controlled release of growth factors for bone tissue engineering.…”

Section: Discussionmentioning

confidence: 99%

3D printed TCP-based scaffold incorporating VEGF-loaded PLGA microspheres for craniofacial tissue engineering

et al. 2017

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Vascularization is a critical process during bone regeneration/repair and the lack of tissue vascularization is recognized as a major challenge in applying bone tissue engineering methods for cranial and maxillofacial surgeries. The aim of our study is to fabricate a vascular endothelial growth factor (VEGF)-loaded gelatin/alginate/β-TCP composite scaffold by 3D printing method using a computer-assisted design (CAD) model. Rheological characterization of various gelatin/alginate/β-TCP formulations led to an optimized paste as a printable bioink at room temperature. VEGF-loaded PLGA microspheres were then incorporated into the paste prior to printing to ensure sustained release of the growth factor. The in vitro release kinetics of the loaded VEGF revealed that the designed scaffolds fulfill the bioavailability of VEGF required for vascularization in the early stages of tissue regeneration. The results were confirmed by two times increment of proliferation of human umbilical vein endothelial cells (HUVECs) seeded on the scaffolds after 10 days. The compressive modulus of the scaffolds, 98 ± 11 MPa, was found to be in the range of cancellous bone suggesting their potential application for craniofacial tissue engineering. Osteoblast culture on the scaffolds showed that the construct supports cell viability, adhesion and proliferation. It was found that the ALP activity increased over 50% using VEGF-loaded scaffolds after 2 weeks of culture. In conclusion, the 3D printed gelatin/alginate/β-TCP scaffold with slow releasing of VEGF can be considered as a potential candidate for regeneration of craniofacial defects.

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Section: Discussionmentioning

confidence: 99%

3D printed TCP-based scaffold incorporating VEGF-loaded PLGA microspheres for craniofacial tissue engineering

et al. 2017

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“…Khojasteh et al., described in their study the fabrication of a three‐dimensional β‐tricalcium‐phosphate/gelatin containing CS‐based nanoparticles for sustained release of bone morphogenetic protein‐2. The study was aimed to assess the possible application of this system for bone tissue engineering.…”

Section: Chitosan and Its Nanoparticlesmentioning

confidence: 99%

Polysaccharide Based Nanoparticles

Khan

Abtew

Modani

et al. 2018

Israel Journal of Chemistry

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Nanoparticles made of biodegradable polymers have outstanding merits as a drug delivery system. Polysaccharide-based nanoparticles have been well explored because of their safety, stability, biocompatibility, biodegradability and hydrophilicity. The present review emphasizes the efforts articulated to improve polysaccharide-based nano-particles as drug delivery tool for effective therapy and sitespecific targeting of mainly anticancer agents. This review updates reports of recent research on polysaccharides-based systems particularly chitosan, alginate, cyclodextrins, hyaluronic acid, pullulan, dextran and their combinations with potential applications.

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“…e need to obtain materials with suitable features for tissue engineering leads to the continued research and development of new process and technologies [1][2][3]. Nowadays, several devices are successfully used in biomedicine.…”

Section: Introductionmentioning

confidence: 99%

PHEMA Hydrogels Obtained by Infrared Radiation for Cartilage Tissue Engineering

Passos

Carvalho

Rodrigues³

et al. 2019

International Journal of Chemical Engineering

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Although the exposure of polymeric materials to radiation is a well-established process, little is known about the relationship between structure and property and the biological behavior of biomaterials obtained by thermal phenomena at 1070 nm wavelength. This study includes results concerning the use of a novel infrared radiation source (ytterbium laser fiber) for the synthesis of poly(2-hydroxyethyl methacrylate) (PHEMA) hydrogel in order to produce medical devices. The materials were obtained by means of free radical polymerization mechanism and evaluated regarding its cross-linking degree, polymer chain mobility, thermal, and mechanical properties. Their potential use as a biomaterial toward cartilage tissue was investigated through incubation with chondrocytes cells culture by dimethylmethylene blue (DMMB) dye and DNA quantification. Differential scanning calorimetry (DSC) results showed that glass transition temperature (Tg) was in the range 103°C–119°C, the maximum degree of swelling was 70.8%, and indentation fluency test presented a strain of 56%–85%. A significant increase of glycosaminoglycans (GAGs) concentration and DNA content in cells cultured with 40 wt% 2-hydroxyethyl methacrylate was observed. Our results showed the suitability of infrared laser fiber in the free radicals formation and in the rapid polymer chain growth, and further cross-linking. The porous material obtained showed improvements concerning cartilage tissue regeneration.

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Fabrication of a three-dimensional β-tricalcium-phosphate/gelatin containing chitosan-based nanoparticles for sustained release of bone morphogenetic protein-2: Implication for bone tissue engineering

Cited by 70 publications

References 55 publications

3D printed TCP-based scaffold incorporating VEGF-loaded PLGA microspheres for craniofacial tissue engineering

3D printed TCP-based scaffold incorporating VEGF-loaded PLGA microspheres for craniofacial tissue engineering

Polysaccharide Based Nanoparticles

PHEMA Hydrogels Obtained by Infrared Radiation for Cartilage Tissue Engineering

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