Fabrication of a novel porous PGA-chitosan hybrid matrix for tissue engineering

Wang, Yu Chi; Lin, Mei Chiao; Wang, Da Ming; Hsieh, Hsyue Jen

doi:10.1016/s0142-9612(02)00434-9

Cited by 139 publications

(84 citation statements)

References 41 publications

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“…Similar endothermic peaks have been reported in the literature for chitosan film. For example, Lima et al [43] and Wang et al [44] reported an endothermic peak of chitosan film at 60.75 o C and 83 o C for fully deacetylated chitosan and 85% DD respectively. As for pure agar, Eldridge and Ferry [45] demonstrated that agar film exhibits an endothermic peak at around 90 o C while Lyons et al [46] found that the agar film shows an endothermic peak at 110 o C which is higher than the usual reported value.…”

Section: Ftir Analysismentioning

confidence: 99%

Preparation and Characterization of Chitosan/Agar Blends: Rheological and Thermal Studies

El-Hefian

Nasef

Yahaya

et al. 2010

J. Chil. Chem. Soc.

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In this work, a number of mixture aqueous solutions of chitosan/agar (CS/AG) at different ratios (considering chitosan as the main component) were prepared. The rheological properties i.e. shearing viscosity and shear stress of the blend solutions as a function of shear rate as well as the thermal properties of the blend films were investigated. Among the parameters studied were temperature, shearing time and storage time. Results showed that almost Newtonian behavior was observed at temperatures from 40 o C to 55 o C for the ratios 100/0, 90/10, 80/20 and 70/30. However, the proportions 60/40 and 50/50 showed a clear shear thinning behavior (pseudoplastic non-Newtonian behavior). It was also found that all the blend solutions obeyed the Arrhenius equation. In addition, the effect of shearing time on the shearing viscosity of all blends did not show any significant differences at all shearing times applied in this study except the proportion 50/50 wherein decreasing in shearing viscosity and shear stress was observed as the shearing time increased. Furthermore, different behaviors were observed for the blend solutions when the period of storage was extended to three weeks. The FTIR results and the differential scanning calorimetery (DSC) curves showed that the interaction between chitosan and agar can occur.

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Section: Ftir Analysismentioning

confidence: 99%

Preparation and Characterization of Chitosan/Agar Blends: Rheological and Thermal Studies

El-Hefian

Nasef

Yahaya

et al. 2010

J. Chil. Chem. Soc.

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“…However, the property of chitosan to support the different cells, the donor, and the load cell phenotype, deacetylation of chitosan molecular weight, and method of sterilisation may affect the results (6,23,24).…”

Section: Discussionmentioning

confidence: 99%

“…Wang et al (24) reported an increased degree of fibroblastic proliferation by using hybrid porous matrices, as a mixture of lowtoxicity solvents and polyglycolide (PGA)-chitosan. We also observed this positive effect of the porous nature of chitosan in the present study.…”

Section: Discussionmentioning

confidence: 99%

“…However, the property of chitosan to support the different cells, the donor and the load cell phenotype, deacetylation of chitosan molecular weight and method of sterilisation may have an effect on the results (6,23,24). Swiss 3T3 mouse embryonic fibroblast cells used in this sense, showing pre-compliance with the terms of the culture experiments, both in the experimental stage and the convenience of use in this regard, led to identification of the molecular structure of the organic material chitosan.…”

Section: Discussionmentioning

confidence: 99%

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Differences between Solution and Membrane Forms of Chitosan on the In Vitro Activity of Fibroblasts

Uslu¹,

Biltekin²,

Denir³

et al. 2015

Balkan Med J

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Background: Chitosan, a linear polysaccharide, has been recently used in biomedical applications. In vitro studies have demonstrated its effect on cellular growth and its stimulatory action on cellular layer formation. Aims: The present study aims to compare the proliferative effects of chitosan in two forms, membranous and solution forms, on Swiss 3T3 mouse embryonic fibroblasts. Study Design: In vitro study. Methods: Three experimental groups were formed: cells were cultured in a normal medium without chitosan (Control Group); cells were cultured either in a medium containing 2.0% chitosan in membranous form (Membrane Group) or chitosan solution at a concentration of 2.0% (Solution Group). Two different methods were used in the experiments: cells cultured on the medium containing chitosan in solution or membranous forms (method 1); and chitosan solution or membranous forms were added into the medium containing previously cultured cells (method 2). Results: Scanning electron microscopic investigations of the experimental groups revealed cells with welldefined cellular projections, intact cellular membranes and tight intercellular junctions. They were especially prominent in the membrane group of method 1 and in the membrane and solution groups of method 2. Mouse monoclonal anti-collagen 1 primary antibody was used to indicate collagen synthesis. Prominent collagen synthesis was detected in the membrane groups on the 10 th day of culture for both methods. Bromodeoxyuridine (BrdU) and MTT assays were performed in order to assess cellular proliferation and viability, respectively. BrdU labelling tests indicated a higher proliferation index in the membrane group of method 1 on the 5 th and 10 th days. For the second method, the membranous form on the 10 th day and solution form on the 5 th day were the most effective groups in terms of cellular proliferation. MTT results reflected a high cellular viability in method 1 on the 5 th day of treatment with the membranous form, whereas cellular viability was highest in the solution form of method 2 on the 5 th day. Conclusion: The membranous form of chitosan induced a significant proliferative effect and increased the ratio of cell-to-cell junctions of Swiss 3T3 mouse embryonic fibroblasts. Conveniently, the solution form also resulted in enhanced cell proliferation and viability compared to the control group. As the solution form is easy to prepare and apply to cells compared to the membrane form, the application of Chitosan directly to media appears to be a convenient alternative for tissue engineering approaches.

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“…These biopolymers also have low to xicity reactions with the body and their degradation rate can be easily controlled. Examp les of synthetic biodegradable polymers include Poly (lact ic acid), PLA [57][58][59][60][61][62], Po ly (L-lactic acid), PLLA [63][64][65][66], Po ly (lactic-co-glycolic acid), PLGA [67][68][69][70], Poly-capro lactone PCL [71][72][73][74] and Poly (g lycolic acid ), PGA [75][76][77][78]. These biopolymers are generally poly-α-hydro x esters that de-esterifies in the body as the polymer degrades to simp le metabolites [79].…”

Section: Introductionmentioning

confidence: 99%

Biomedical Magnesium Alloys: A Review of Material Properties, Surface Modifications and Potential as a Biodegradable Orthopaedic Implant

Poinern¹,

Brundavanam²,

Fawcett³

2013

AJBE

262

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Magnesium and magnesium based alloys are lightweight metallic materials that are extremely biocompatib le and have similar mechanical properties to natural bone. These materials have the potential to function as an osteoconductive and biodegradable substitute in load bearing applicat ions in the field of hard t issue engineering. However, the effects of corrosion and degradation in the physiological environ ment of the body has prevented their wide spread applicat ion to date. The aim o f this review is to examine the properties, chemical stability, degradation in situ and methods of improving the corrosion resistance of magnesium and its alloys for potential application in the orthopaedic field. To be an effective imp lant, the surface and sub-surface properties of the material needs to be carefully selected so that the degradation kinetics of the implant can be efficiently controlled. Several surface modification techniques are presented and their effectiveness in reducing the corrosion rate and methods of controlling the degradation period are discussed. Ideally, balancing the gradual loss of material and mechanical strength during degradation, with the increasing strength and stability of the newly forming bone tissue is the ultimate goal. If this goal can be achieved, then orthopaedic implants manufactured fro m magnesium based alloys have the potential to deliver successful clinical outcomes without the need for revision surgery.

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Fabrication of a novel porous PGA-chitosan hybrid matrix for tissue engineering

Cited by 139 publications

References 41 publications

Preparation and Characterization of Chitosan/Agar Blends: Rheological and Thermal Studies

Preparation and Characterization of Chitosan/Agar Blends: Rheological and Thermal Studies

Differences between Solution and Membrane Forms of Chitosan on the In Vitro Activity of Fibroblasts

Biomedical Magnesium Alloys: A Review of Material Properties, Surface Modifications and Potential as a Biodegradable Orthopaedic Implant

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