Fabrication of a colorimetric sensor using acetic acid-capped drug-mediated copper oxide nanoparticles for nitrite biosensing in processed food

Abstract

Nitrite (NO2−) and nitrate (NO3−) are frequently used in cured meat products as preservatives, as they give a better taste and work well in color fixation. As a key possible carcinogen, excessive dietary consumption of NO2− in cured meat products would be bad for health. Herein, copper oxide nanoparticles (CuO NPs) were synthesized using the drug Augmentin as a reducing and capping agent. The desired synthesis of CuO NPs was confirmed by various characterization techniques, including UV–visible spectroscopy, Fourier transform infrared spectroscopy, energy-dispersive X-ray spectroscopy, thermal gravimetric analysis, X-ray diffraction, and scanning electron microscopy. The new approach of drug-mediated acetic acid-capped CuO NPs was developed for simple colorimetric detection of nitrite ions in a mimic solution of processed food. The color of the detection system changes from brown to yellow with the increase in the concentration of NO2− and has been observed with the naked eye. The selectivity of the NO2− detection system by the UV–visible spectrum and the naked eye is compared to other ions, such as Br−, I−, Cl−1, PO4−3, CO32−, and SO42−. The platform was successfully employed for the determination of nitrite in real samples. Moreover, this probe can be used for the sensitive detection of NO2− with a linear range of 1 × 10−8 to 2.40 × 10−6 M, a detection limit of 2.69 × 10−7 M, a limit of quantification 8.9 × 10−7 M, and a regression coefficient (R2) of 0.997. Our results suggest that this sensor can be used for on-site analysis and quantification as well as in the fields of disease diagnosis, environmental monitoring, and food safety.

From waste to wealth: iron oxide doped hydroxyapatite-based biosensor for the colorimetric detection of ascorbic acid

“…Conditions: 4 mg HAp@Fe 2 O 3 , 150 mL TMB (12 mM), 100 mL (24 mM) hydrogen peroxide, and 120 mL ascorbic acid (56 mM). (ii) AA, HAp@Fe 2 O 3 , and TMB solution interaction is demonstrated at different pH levels (3)(4)(5)(6)(7)(8)(9) nanoparticles having a structure with an expanded surface area is highly needed for the catalytic activity of our synthesized platform. By introducing iron into the HAp, we can further decrease the particle size, increase the surface area, and make it a better reactive surface for catalytic activities due to the enrichment of electrons.…”

“…On the other hand, the colorimetric method has great potential for ascorbic acid detection owing to its simplicity, rapid operation, and cost-effectiveness. 5 The added advantage associated with this approach is that its results can be visualized by the unaided eye. 6 Hydroxyapatite (HAp) holds a prime position among biomaterials and has been utilized for bone implants.…”

“…Conditions: 4 mg HAp@Fe 2 O 3 , 150 mL TMB (12 mM), 100 mL (24 mM) hydrogen peroxide, and 120 mL ascorbic acid (56 mM). (ii) AA, HAp@Fe 2 O 3 , and TMB solution interaction is demonstrated at different pH levels(3)(4)(5)(6)(7)(8)(9) in PBS at 4 mg HAp@Fe 2 O 3 [ascorbic acid] = 56 mM (120 mL). The ideal colorimetric response was obtained at a pH of 6.…”