Fabrication of a chitosan/bioglass three-dimensional porous scaffold for bone tissue engineering applications

Yang, Jun; Long, Teng; He, Nanfei; Guo, Ya‐Jun; Zhu, Zhenan; Ke, Qinfei

doi:10.1039/c4tb00940a

Cited by 54 publications

(24 citation statements)

References 53 publications

(51 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, since Philippart et al [101] have recently thoroughly reviewed this specific topic, we shall restrict ourselves to only mention here the most important polymeric materials used for this purpose. The polymeric materials employed in conjunction with bioactive glass-based bone scaffolds can be either synthetical (e.g., polyvinyl alcohol (PVA) [102], poly-DL-lactic acid [108], poly(ε-caprolactone) [103,109], poly(lactic-coglycolic acid) [110], poly( d , l -lactide)/poly(ethylene glycol)-(polypropylene glycol)-poly(ethyleneglycol) [104]), natural (e.g., collagen [105], bacteria-derived poly-(3-hydroxybutyrate) [73] or poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [106], chitosan [111], cellulose [107], alginate [112], silk [113], zein [103], or gelatin [100]) or hybrid (poly(ε-caprolactone)/zein [103]; PVA/microfibrillated cellulose [102]) in origin. The usage of gelatin, as obtained by the temperature or chemical processing of collagen, can alleviate the risk of antigenicity, which is typically associated with animal-origin compounds [100].…”

Section: Bioactive Glasses and Glass-ceramicsmentioning

confidence: 99%

Bioactive Glasses and Glass-Ceramics for Healthcare Applications in Bone Regeneration and Tissue Engineering

et al. 2018

View full text Add to dashboard Cite

The discovery of bioactive glasses (BGs) in the late 1960s by Larry Hench et al. was driven by the need for implant materials with an ability to bond to living tissues, which were intended to replace inert metal and plastic implants that were not well tolerated by the body. Among a number of tested compositions, the one that later became designated by the well-known trademark of 45S5 Bioglass® excelled in its ability to bond to bone and soft tissues. Bonding to living tissues was mediated through the formation of an interfacial bone-like hydroxyapatite layer when the bioglass was put in contact with biological fluids in vivo. This feature represented a remarkable milestone, and has inspired many other investigations aiming at further exploring the in vitro and in vivo performances of this and other related BG compositions. This paradigmatic example of a target-oriented research is certainly one of the most valuable contributions that one can learn from Larry Hench. Such a goal-oriented approach needs to be continuously stimulated, aiming at finding out better performing materials to overcome the limitations of the existing ones, including the 45S5 Bioglass®. Its well-known that its main limitations include: (i) the high pH environment that is created by its high sodium content could turn it cytotoxic; (ii) and the poor sintering ability makes the fabrication of porous three-dimensional (3D) scaffolds difficult. All of these relevant features strongly depend on a number of interrelated factors that need to be well compromised. The selected chemical composition strongly determines the glass structure, the biocompatibility, the degradation rate, and the ease of processing (scaffolds fabrication and sintering). This manuscript presents a first general appraisal of the scientific output in the interrelated areas of bioactive glasses and glass-ceramics, scaffolds, implant coatings, and tissue engineering. Then, it gives an overview of the critical issues that need to be considered when developing bioactive glasses for healthcare applications. The aim is to provide knowledge-based tools towards guiding young researchers in the design of new bioactive glass compositions, taking into account the desired functional properties.

show abstract

Section: Bioactive Glasses and Glass-ceramicsmentioning

confidence: 99%

Bioactive Glasses and Glass-Ceramics for Healthcare Applications in Bone Regeneration and Tissue Engineering

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Chitosan (CS), a N -deacetylated chitin derivative, is a promising material for bone tissue engineering owing to its structure resemblance to the glycosaminoglycans (GAGs) found in natural bones and cartilage [ 8 , 9 ]. It has been characterized with exceptional biocompatibility, biodegradability, non-toxicity [ 10 ], as well as antimicrobial activities [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Preliminary In Vitro Evaluation of Chitosan–Graphene Oxide Scaffolds on Osteoblastic Adhesion, Proliferation, and Early Differentiation

Wong

Lim

Tiong

et al. 2020

IJMS

View full text Add to dashboard Cite

An ideal scaffold should be biocompatible, having appropriate microstructure, excellent mechanical strength yet degrades. Chitosan exhibits most of these exceptional properties, but it is always associated with sub-optimal cytocompatibility. This study aimed to incorporate graphene oxide at wt % of 0, 2, 4, and 6 into chitosan matrix via direct blending of chitosan solution and graphene oxide, freezing, and freeze drying. Cell fixation, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, alkaline phosphatase colorimetric assays were conducted to assess cell adhesion, proliferation, and early differentiation of MG63 on chitosan–graphene oxide scaffolds respectively. The presence of alkaline phosphatase, an early osteoblast differentiation marker, was further detected in chitosan–graphene oxide scaffolds using western blot. These results strongly supported that chitosan scaffolds loaded with graphene oxide at 2 wt % mediated cell adhesion, proliferation, and early differentiation due to the presence of oxygen-containing functional groups of graphene oxide. Therefore, chitosan scaffolds loaded with graphene oxide at 2 wt % showed the potential to be developed into functional bone scaffolds.

show abstract

“…Ideal scaffolds for bone tissue engineering should have the following properties: (i) biocompatibility; they should satisfy the common demands of medical materials, which are not toxic and do not degrade into toxic matters, without teratogenicity and tumorigenicity; (ii) biodegradability, in that they can degrade in accordance with tissue restoration, and that the biodegradability is tunable; (iii) good osteoconductivity, in that they can promote adhesion, growth, and related biological properties, and they are helpful for the import of oxygen and export of carbon dioxide, additionally, they can promote an ingrowth of vessels and nerves; (iv) plasticity, the materials can be fabricated into scaffolds of needed porosity and type, in addition, they should have considerable mechanical strength and fatigue resistance, so as to provide mechanical support; and (v) easy to obtain and at a low cost [ 12 , 13 , 14 , 15 ]. All in all, ideal scaffolds can create an environment in which cells can biologically adapt to them.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Gelatin/PCL Electrospun Fiber Mat with Bone Powder and the Study of Its Biocompatibility

Rong

Chen

Yang

et al. 2016

JFB

View full text Add to dashboard Cite

Fabricating ideal scaffolds for bone tissue engineering is a great challenge to researchers. To better mimic the mineral component and the microstructure of natural bone, several kinds of materials were adopted in our study, namely gelatin, polycaprolactone (PCL), nanohydroxyapatite (nHA), and bone powder. Three types of scaffolds were fabricated using electrospinning; gelatin/PCL, gelatin/PCL/nHA, and gelatin/PCL/bone powder. Scaffolds were examined using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) observations. Then, Adipose-derived Stem Cells (ADSCs) were seeded on these scaffolds to study cell morphology, cell viability, and proliferation. Through this study, we found that nHA and bone powder can be successfully united in gelatin/PCL fibers. When compared with gelatin/PCL and gelatin/PCL/nHA, the gelatin/PCL/bone powder scaffolds could provide a better environment to increase ADSCs’ growth, adhesion, and proliferation. Thus, we think that gelatin/PCL/bone powder has good biocompatibility, and, when compared with nHA, bone powder may be more effective in bone tissue engineering due to the bioactive factors contained in it.

show abstract

Fabrication of a chitosan/bioglass three-dimensional porous scaffold for bone tissue engineering applications

Abstract: A chitosan/bioglass three-dimensional porous scaffold with excellent biocompatibility and mechanical properties has been developed for the treatment of bone defects.

Cited by 54 publications

References 53 publications

Bioactive Glasses and Glass-Ceramics for Healthcare Applications in Bone Regeneration and Tissue Engineering

Bioactive Glasses and Glass-Ceramics for Healthcare Applications in Bone Regeneration and Tissue Engineering

Preliminary In Vitro Evaluation of Chitosan–Graphene Oxide Scaffolds on Osteoblastic Adhesion, Proliferation, and Early Differentiation

Fabrication of Gelatin/PCL Electrospun Fiber Mat with Bone Powder and the Study of Its Biocompatibility

Contact Info

Product

Resources

About