Fabrication of 3D cylindrical thermosensitive hydrogels as supports for cell culture and detachment of tubular cell sheets

García-Sobrino, Rubén; Lago, Eugenia; Goñi, Clara; Ramos, Viviana; García, Carlos Tejerizo; Reinecke, Helmut; Elvira, Carlos; Rodríguez‐Hernández, Juan; Gallardo, Alberto; Martínez-Campos, Enrique

doi:10.1016/j.bioadv.2022.213210

Cited by 2 publications

(1 citation statement)

References 29 publications

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“…This difference is accentuated in the early stages of the test (see magnification in Figure 4b), indicating the rapid evaporation of the mixture due to the volatility of the solvent. Given the fairness in terms of chemical composition of the ink, the importance of the amount expelled after the extrusion process is observed, where the samples with a lower amount of EPS-ink lose a higher mass percentage, demonstrating the importance of the geometry of the specimen in kinetic studies by gravimetry, as other authors have shown [30]. Here, the evaporation process begins on the surface of the printed ribbons, generating a thin layer that hinders the evaporation process of the remaining acetone.…”

Section: D Printing Parameter Optimizationmentioning

confidence: 55%

Novel and Accessible Physical Recycling for Expanded Polystyrene Waste with the Use of Acetone as a Solvent and Additive Manufacturing (Direct Ink-Write 3D Printing)

García-Sobrino,

Cortés,

Calderón-Villajos

et al. 2023

Polymers

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The current high production of plastics has prompted the exploration of alternative pathways to facilitate recycling, aiming for a progressively sustainable society. This paper presents an alternative and affordable technology for treating waste expanded polystyrene (EPS) mixed with acetone in a 100:1 volume ratio to be used as 3D printing ink for Direct Ink Write technology. In order to optimize the printing parameters, a comprehensive study was conducted, evaluating different needle diameters, printing speeds, and bed temperature values to achieve homogenous pieces and a highly repeatable 3D printing process. Results showed that the main optimum printing parameters were using needles with diameters of 14 to 16 G and printing speeds ranging from 2 to 12 mm/s, which were found to yield the most uniform ribbons. Increasing the bed temperature, despite favoring acetone evaporation, led to the generation of more heterogeneous structures due to void growth inside the printed ribbons. Thus, employing room temperature for the bed proved to be the optimal value. Lastly, a comparative study between the starting material and the EPS after the printing process was conducted using FTIR-ATR and GPC analyses, ensuring the preservation of the original polymer’s integrity during physical recycling.

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Section: D Printing Parameter Optimizationmentioning

confidence: 55%

Novel and Accessible Physical Recycling for Expanded Polystyrene Waste with the Use of Acetone as a Solvent and Additive Manufacturing (Direct Ink-Write 3D Printing)

García-Sobrino,

Cortés,

Calderón-Villajos

et al. 2023

Polymers

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Osteoblastic Cell Sheet Engineering Using P(VCL-HEMA)-Based Thermosensitive Hydrogels Doped with pVCL@Icariin Nanoparticles Obtained with Supercritical CO2-SAS

García-Sobrino,

Casado-Losada,

Caltagirone

et al. 2024

Pharmaceutics

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New clinical strategies for treating severe bone and cartilage injuries are required, especially for use in combination with implant procedures. For this purpose, p(VCL-co-HEMA) thermosensitive hydrogels have been activated with icariin-loaded nanoparticles to be used as bone-cell-harvesting platforms. Supercritical CO2-SAS technology has been applied to encapsulate icariin, a small molecule that is involved in osteoblastic differentiation. Thus, physical-chemical analysis, including swelling and transmittance, showed the impact of HEMA groups in hydrogel composition. Moreover, icariin (ICA) release from p(VCL-co-HEMA) platforms, including pVCL@ICA nanoparticles, has been studied to evaluate their efficacy in relevant conditions. Finally, the thermosensitive hydrogels’ cell compatibility, transplant efficiency, and bone differentiation capacity were tested. This study identifies the optimal formulations for icariin-activated hydrogels for both control and HEMA formulations. Using this technique, osteoblastic sheets that were rich in collagen type I were successfully transplanted and recultivated, maintaining an optimal extracellular matrix (ECM) composition. These findings suggest a new cell-sheet-based therapy for bone regeneration purposes using customized and NP-activated pVCL-based cell platforms.

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Fabrication of 3D cylindrical thermosensitive hydrogels as supports for cell culture and detachment of tubular cell sheets

Cited by 2 publications

References 29 publications

Novel and Accessible Physical Recycling for Expanded Polystyrene Waste with the Use of Acetone as a Solvent and Additive Manufacturing (Direct Ink-Write 3D Printing)

Novel and Accessible Physical Recycling for Expanded Polystyrene Waste with the Use of Acetone as a Solvent and Additive Manufacturing (Direct Ink-Write 3D Printing)

Osteoblastic Cell Sheet Engineering Using P(VCL-HEMA)-Based Thermosensitive Hydrogels Doped with pVCL@Icariin Nanoparticles Obtained with Supercritical CO2-SAS

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