Fabrication, characterization and fibroblast proliferative activity of electrospun Achillea lycaonica-loaded nanofibrous mats

Çam, Muhammet Emin; Cesur, Sümeyye; Taşkın, Turgut; Erdemir, Gökçe; Erdem‐Kuruca, Serap; Şahin, Yeşim Müge; Kabasakal, Levent; Gündüz, Oğuzhan

doi:10.1016/j.eurpolymj.2019.109239

Cited by 30 publications

(15 citation statements)

References 34 publications

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“…The main parameters that affect the formation and homogeneity of nanofibrous scaffolds are ambient conditions such as temperature and humidity, process conditions such as working pressure and rotational speed for PG; working distance, polymer solution feed rate, applied voltage for electrospinning, solution properties such as viscosity, density, surface tension, and in addition for electrospinning, electrical conductivity. [47,48] These parameters were recorded for each solution (Table S1a).…”

Section: Physical Properties Of Solutionsmentioning

confidence: 99%

Accelerated diabetic wound healing by topical application of combination oral antidiabetic agents-loaded nanofibrous scaffolds: An in vitro and in vivo evaluation study

Çam

Ertaş

Alenezi

et al. 2021

Materials Science and Engineering: C

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The combination of oral antidiabetic drugs, pioglitazone, metformin, and glibenclamide, which also exhibit the strongest anti-inflammatory action among oral antidiabetic drugs, were loaded into chitosan/gelatin/polycaprolactone (PCL) by electrospinning and polyvinyl pyrrolidone (PVP)/PCL composite nanofibrous scaffolds by pressurized gyration to compare the diabetic wound healing effect. The combination therapies significantly accelerated diabetic wound healing in type-1 diabetic rats and organized densely packed collagen fibers in the dermis, it also showed better regeneration of the dermis and epidermis than single drug-loaded scaffolds with less inflammatory cell infiltration and edema. The formation of the hair follicles started in 14 days only in the combination therapy and lower proinflammatory cytokine levels were observed compared to single drug-loaded treatment groups. The combination therapy increased the wettability and hydrophilicity of scaffolds, demonstrated sustained drug release over 14 days, has high tensile strength and suitable cytocompatibility on L929 (mouse fibroblast) cell and created a suitable area for the proliferation of fibroblast cells. Consequently, the application of metformin and pioglitazone-loaded chitosan/gelatin/PCL nanofibrous scaffolds to a diabetic wound area offer high bioavailability, fewer systemic side effects, and reduced frequency of dosage and amount of drug.

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Section: Physical Properties Of Solutionsmentioning

confidence: 99%

Accelerated diabetic wound healing by topical application of combination oral antidiabetic agents-loaded nanofibrous scaffolds: An in vitro and in vivo evaluation study

Çam

Ertaş

Alenezi

et al. 2021

Materials Science and Engineering: C

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“…The Achillea genus, one of the most important genera of the Asteraceae family with ethnopharmacological significance, consists of approximately 85 species mainly distributed in Middle East regions, such as Iran, Turkey, and Serbia and Eastern regions of Europe [ 1 ]. Achillea species have been reported to possess highly bioactive compounds and were rich in flavones and other flavonoids [ 2 ], non-saturated carboxylic acids [ 3 ], phenolic glycosides [ 4 ], guaianolides [ 5 ], lignans [ 6 ], phthalate derivatives [ 7 ], piperidine amides, proazulenes [ 8 ], sesquiterpenes [ 9 ], sesquiterpene lactone-diol [ 10 ], sesquiterpene lactones [ 11 ], polyacetylenes [ 12 ], spirodepressolide [ 13 ], tannins [ 14 ], and triterpene alkamide [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…An ethnobotanical survey conducted by Mohammadnoseini and colleagues (2017) highlighted the use of several Achillea species in traditional medicine for the management of several ailments [ 16 ]. In addition, pharmacological studies have demonstrated that various Achillea species possess biological activities, such as antioxidant, antibacterial, antispasmodic, and anti-inflammatory [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

UHPLC-MS Characterization and Biological Insights of Different Solvent Extracts of Two Achillea Species (A. aleppica and A. santolinoides) from Turkey

et al. 2021

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In the current study, Achillea santolinoides and Achillea aleppica aeral parts and root were extracted with ethyl acetate, methanol, and water. Detailed phytochemical profiles were obtained using UHPLC-MS, yielding the identification of hydroxybenzoic and hydroxycinnamic acids, phenolic acid glycosides and sugar esters, acylquinic acids, O-glycosyl flavones and flavonols, and flavonoid aglycons, among others. The antioxidant properties and enzyme inhibitory activities of the extracts were assayed with in vitro tests. The phenolic content of the water extracts was significantly higher as compared to the ethyl acetate and methanol ones. A. aleppica aerial parts methanol extract possessed highest flavonoid content (49.18 mg rutin equivalent/g). Antioxidant properties assessment revealed that the methanol extract of A. santolinoides roots actively scavenged DPPH (54.11 mg TE/g) and ABTS radicals (112.53 mg TE/g) and possessed highest reducing potential (183.55 and 129.92 mg TE/g, for CUPRAC and FRAP, respectively). The ethyl acetate extracts of aerial parts and roots of both species showed highest inhibition against BuCHE (6.07–6.76 mg GALAE/g). The ethyl acetate extract of A.santolinoides aerial part showed highest inhibition against tyrosinase (73.00 mg KAE/g). These results showed that the tested Achillea species might represent novel phytotherapeutic avenues for the management of Alzheimer’s disease and epidermal hyperpigmentation conditions, which are both associated with oxidative stress. This paper could shed light into future potential industrial applications using the tested Achillea species.

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“…In recent years, the study of controlled release of drugs and bioactive agents from polymeric materials has attracted the attention of many researchers from around the world [3]. Controlled drug delivery applications include both sustained delivery over days/weeks/months/years and targeted delivery on a single or sustained basis [4].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of burst release and sustained release of pioglitazone-loaded fibrous mats on diabetic wound healing: an in vitro and in vivo comparison study

Çam

Yildiz

Alenezi

et al. 2020

J. R. Soc. Interface.

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In order to provide more effective treatment strategies for the rapid healing of diabetic wounds, novel therapeutic approaches need to be developed. The therapeutic potential of peroxisome proliferator-activated receptor-γ (PPAR-γ) agonist pioglitazone hydrochloride (PHR) in two different release kinetic scenarios, burst release and sustained release, was investigated and compared with in vitro and in vivo tests as potential wound healing dressings. PHR-loaded fibrous mats were successfully fabricated using polyvinyl-pyrrolidone and polycaprolactone by scalable pressurized gyration. The results indicated that PHR-loaded fibrous mats expedited diabetic wound healing in type-1 diabetic rats and did not show any cytotoxic effect on NIH/3T3 (mouse embryo fibroblast) cells, albeit with different release kinetics and efficacies. The wound healing effects of fibrous mats are presented with histological and biochemical evaluations. PHR-loaded fibrous mats improved neutrophil infiltration, oedema, and inflammation and increased epidermal regeneration and fibroblast proliferation, but the formation of hair follicles and completely improved oedema were observed only in the sustained release form. Thus, topical administration of PPAR-γ agonist in sustained release form has high potential for the treatment of diabetic wounds in inflammatory and proliferative phases of healing with high bioavailability and fewer systemic side effects.

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Fabrication, characterization and fibroblast proliferative activity of electrospun Achillea lycaonica-loaded nanofibrous mats

Cited by 30 publications

References 34 publications

Accelerated diabetic wound healing by topical application of combination oral antidiabetic agents-loaded nanofibrous scaffolds: An in vitro and in vivo evaluation study

Accelerated diabetic wound healing by topical application of combination oral antidiabetic agents-loaded nanofibrous scaffolds: An in vitro and in vivo evaluation study

UHPLC-MS Characterization and Biological Insights of Different Solvent Extracts of Two Achillea Species (A. aleppica and A. santolinoides) from Turkey

Evaluation of burst release and sustained release of pioglitazone-loaded fibrous mats on diabetic wound healing: an in vitro and in vivo comparison study

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