Fabrication, characterization and cellular biocompatibility of porous biphasic calcium phosphate bioceramic scaffolds with different pore sizes

Tang, Xuehui; Mao, Liang; Liu, Jiaqiang; Liu, Yang; Zhang, Wei; Shu, Mengjun; Hu, Nantao; Jiang, Lingyong; Fang, Bing

doi:10.1016/j.ceramint.2016.06.172

Cited by 13 publications

(5 citation statements)

References 34 publications

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“…BCPs are composed of calcium phosphate, the same inorganic material which comprises the natural skeleton (12,13,15). The present study suggested that tissue-engineered bone constructed with BCP scaffolds could successfully repair the bone defect, and BCP could be absorbed in vivo (40,41). These results validated earlier studies that reported that BCP is the ideal choice of scaffolds for tissue-engineered bone (12,13,15).…”

Section: Discussionmentioning

confidence: 65%

The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects

et al. 2019

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Although miRNAs have been implicated in the osteogenic differentiation of stem cells, their role in bone repair and reconstruction in tissue-engineered bone grafts remains unclear. We previously reported that microRNA (miR)-26a-5p inhibited the osteogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs), and that antimiR-26a-5p exerted the opposite effect. In the present study, the role of miR-26a-5p- and antimiR-26a-5p-modified ADSCs combined with biphasic calcium phosphate (BCP) scaffolds was evaluated in a rat femur defect model. The aim of the present study was to improve the understanding of the role of miR-26a-5p in bone regeneration in vivo , as well as to provide a new method to optimize the osteogenic ability of BCPs. ADSCs were infected with Lv-miR-26a-5p, Lv-miR-NC, Lv-antimiR-26a-5p or Lv-antimiR-NC respectively, and then combined with BCP scaffolds to repair rat femoral defects. Using X-rays, micro-computed tomography and histology at 2, 4, and 8 weeks postoperatively, the quantity and rate of bone regeneration were analyzed, revealing that they were the highest in animals treated with antimiR-26a-5p and the lowest in the miR-26a-5p treatment group. The expression levels of osteocalcin, collagen I, Runt-related transcription factor 2, Wnt family member 5A and calmodulin-dependent protein kinase II proteins were positively correlated with the bone formation rate. Taken together, the present results demonstrated that miR-26a-5p inhibited bone formation while antimiR-26a-5p accelerated bone formation via the Wnt/Ca 2+ signaling pathway. Therefore, antimiR-26a-5p-modified ADSCs combined with BCP scaffolds may be used to construct an effective tissue-engineering bone graft for bone repair and reconstruction.

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Section: Discussionmentioning

confidence: 65%

The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects

et al. 2019

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“…The results indicate that the average macropore diameter ranges from 100 to 600 μm, based on the amount of sodium dodecyl sulfate (SDS) from 0.8 to 0.5 g, respectively. The BCP scaffold has shown excellent degradability performance in SBF solution when incubated for up to 49 days . Seo et al.…”

Section: Biocompatibility and Bioresorbability Studies On Additive Ma...mentioning

confidence: 99%

Review of Physical, Mechanical, and Biological Characteristics of 3D-Printed Bioceramic Scaffolds for Bone Tissue Engineering Applications

Thangavel

Elsen

2022

ACS Biomater. Sci. Eng.

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This review focuses on the advancements in additive manufacturing techniques that are utilized for fabricating bioceramic scaffolds and their characterizations leading to bone tissue regeneration. Bioscaffolds are made by mimicking the human bone structure, material composition, and properties. Calcium phosphate apatite materials are the most commonly used scaffold materials as they closely resemble live bone in their inorganic composition. The functionally graded scaffolds are fabricated by utilizing the right choice of the 3D printing method and material combinations to achieve the requirement of the bioscaffold. To tailor the physical, mechanical, and biological properties of the scaffold, certain materials are reinforced, doped, or coated to incorporate the functionality. The biomechanical loading conditions that involve flexion, torsion, and tension exerted on the implanted scaffold are discussed. The finite element analysis (FEA) technique is used to investigate the mechanical property of the scaffold before fabrication. This helps in reducing the actual number of samples used for testing. The FEA simulated results and the experimental result are compared. This review also highlights some of the challenges associated while processing the scaffold such as shrinkage, mechanical instability, cytotoxicity, and printability. In the end, the new materials that are evolved for tissue engineering applications are compiled and discussed.

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“…Despite these characteristics, HAp has poor biodegradability where it may remain integrated into the regenerated bone tissue once implemented into the body. 1 Contrary to HAp, β-TCP degrades faster without osteoclastic resorption indicating that the rate of β-TCP resorption does not correspond to that of bone formation. 2 The imbalance between resorption and osteogenesis results in the deterioration of the bone quality.…”

Section: Introductionmentioning

confidence: 95%

Effect of Soaking Time on the Phase Transformation of Porous Biphasic β-Tricalcium Phosphate/Carbonate Apatite Scaffold

Muhaime¹,

Artillia²,

Ahmad³

2019

JPS

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Fabrication, characterization and cellular biocompatibility of porous biphasic calcium phosphate bioceramic scaffolds with different pore sizes

Cited by 13 publications

References 34 publications

The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects

The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects

Review of Physical, Mechanical, and Biological Characteristics of 3D-Printed Bioceramic Scaffolds for Bone Tissue Engineering Applications

Effect of Soaking Time on the Phase Transformation of Porous Biphasic β-Tricalcium Phosphate/Carbonate Apatite Scaffold

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