Fabrication, Assembly, and Application of Patchy Particles

Pawar, Amar B.; Kretzschmar, Ilona

doi:10.1002/marc.200900614

Cited by 438 publications

(412 citation statements)

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“…Given that both natural proteins 3-8 and synthetic colloids [18][19][20][21][22][23][24][25][26][27][28][29] have been shown to readily assemble into symmetric, higher order structures, we anticipate that our SuPrA strategy can now be generalized to create synthetic, scalable…”

Section: 31mentioning

confidence: 99%

“…Notably, while some GFP variants, including the parent protein originally found in Aeqorea Victoria dimerize, the native dimer structure fits poorly into the electron density maps of the protomer, suggesting that the architecture of the protomer is completely novel. 54 The formation of SuPrA protomers appears to be largely independent of the placement of charges, suggesting that oppositely supercharging protein variants may generally drive formation into 'stacked' or brick-like structures whose overall form is determined by symmetric interactions.Given that both natural proteins 3-8 and synthetic colloids [18][19][20][21][22][23][24][25][26][27][28][29] have been shown to readily assemble into symmetric, higher order structures, we anticipate that our SuPrA strategy can now be generalized to create synthetic, scalableAll rights reserved. No reuse allowed without permission.…”

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confidence: 99%

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Supercharging enables organized assembly of synthetic biomolecules

Simon

Ramasubramani

Gläser

et al. 2018

Preprint

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Abstract:There are few methods for the assembly of defined protein oligomers and higher order structures that could serve as novel biomaterials. Using fluorescent proteins as a model system, we have engineered novel oligomerization states by combining oppositely supercharged variants. A well-defined, highly symmetrical 16-mer (two stacked, circular octamers) can be formed from alternating charged proteins; higher order structures then form in a hierarchical fashion from this discrete protomer. During SUpercharged PRotein Assembly (SuPrA), electrostatic attraction between oppositely charged variants drives interaction, while shape and patchy physicochemical interactions lead to spatial organization along specific interfaces, ultimately resulting in protein assemblies never before seen in nature.All rights reserved. No reuse allowed without permission.(which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint . http://dx.doi.org/10.1101/323261 doi: bioRxiv preprint first posted online May. 16, 2018; 2 The assembly of genetically-encoded molecules into symmetric, supramolecular materials enables complex functions in natural biosystems and would likewise prove valuable in the development of bionanotechnologies including drug delivery, energy transport, and biological information storage. [1][2][3][4][5][6][7] Repeated, symmetric interactions between molecular building blocks enable the formation of complex, defined, assemblies from a small total number of components as well as dynamic propagation of conformational change. [3][4][5][6][7][8] However, while de novo engineering of artificial symmetrical biomolecular complexes has begun to be explored, 9-15 these efforts generally rely upon precise design of molecular interfaces. 16,17 In contrast, studies of simple synthetic colloids suggests that higher order structures can be derived based solely on packing and energetic and considerations. Computational and experimental studies of polyhedral or spherical colloids indicate that complementary particle shapes 18-24 and simple attractive "patches" [24][25][26][27][28][29] alone can enable formation of complex symmetrical assemblies. Shape complementarity has likewise been exploited to arrange synthetic linear biomolecules, i.e., double-stranded DNA strands, into distinct structures, demonstrating its utility for for the higher order arrangement of synthetic linear biomolecules, suggesting a utility beyond inorganic systems. 30,31Herein we demonstrate a simple, robust strategy to assemble normally monomeric proteins into well defined, oligomeric quaternary structures and micron-scale particles. This strategy centers on driving protein interactions by engineering oppositely supercharged variants. Generally, oppositely-charged proteins interact through simple electrostativ interactions. 32 Previously, this propensity has been exploited in artificial biosystems to engineer binary protein crystals from naturally oppos...

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Section: 31mentioning

confidence: 99%

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confidence: 99%

Supercharging enables organized assembly of synthetic biomolecules

Simon

Ramasubramani

Gläser

et al. 2018

Preprint

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“…Janus particles [89,90], which consist of two faces of differing surface chemistry, or (more generally) particles with patchy surface morphologies [91] increase the complexity of materials that can be formed from assembly of the constituent building blocks. This is because by breaking the symmetry of the particle surface chemistry, inter-particle interactions are now not just dependent upon their relative [92]; lipid mixtures can therefore be developed that phase separate into two or more of these coexisting phase textures [93][94][95][96].…”

Section: Breaking Symmetry: Phase Separation and Aspherical Structmentioning

confidence: 99%

Application of nucleic acid–lipid conjugates for the programmable organisation of liposomal modules

Beales

Vanderlick

2014

Advances in Colloid and Interface Science

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“…However, only two patches are made using this method, and low quantities of particles are obtained. Other strategies have used faceted particles 24 , particles with protrusions 25 or coordinated patches 26,27,28 , but 3-dimensional directional bonding and assembly have yet to be demonstrated 29 .…”

Section: Introductionmentioning

confidence: 99%

Colloids with valence and specific directional bonding

Wang

Breed

et al. 2012

Nature

955

1,072

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The ability to design and assemble 3-dimensional structures from colloidal particles is limited by the absence of specific directional bonds. As a result, complex or low-coordination structures, common in atomic and molecular systems, are rare in the colloidal domain. Here we demonstrate a general method for creating the colloidal analogues of atoms with valence: colloidal particles with chemically functionalized patches that can form highly directional bonds.These "colloidal atoms" possess all the common symmetries-and some uncommon ones-characteristic of hybridized atomic orbitals, including sp, sp 2 , sp 3 , sp 3 d, sp 3 d 2 , and sp 3 d 3 . Functionalizing the patches with DNA with single-stranded sticky ends makes the interactions between patches on different particles programmable, specific, and reversible, thus facilitating the self-assembly of particles into "colloidal molecules," including "molecules" with triangular, tetrahedral, and other bonding symmetries. Because colloidal dynamics are slow, the kinetics of molecule formation can be followed directly by optical microscopy. These new colloidal atoms should enable the assembly of a rich variety of new micro-structured materials. 2 IntroductionThe past decade has seen an explosion in the kinds of colloidal particles that can be synthesized 1,2 , with many new shapes, such as cubes 3 , clusters of spheres 4-6 and dimpled particles 7,8 reported. Because the self-assembly of these particles is largely controlled by their geometry, only a few relatively simple crystals have been made: face-centered and body-centered cubic crystals and variants 9 . Colloidal alloys increase the diversity of structures [10][11][12] , but many structures remain difficult or impossible to make. For example, the diamond lattice, predicted more than 20 years ago to have a full 3-dimensional photonic band gap 13 , still cannot be made by colloidal self-assembly because it requires 4-fold coordination. Without directional bonds, such low-coordination states are unstable.

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Fabrication, Assembly, and Application of Patchy Particles

Cited by 438 publications

References 166 publications

Supercharging enables organized assembly of synthetic biomolecules

Supercharging enables organized assembly of synthetic biomolecules

Application of nucleic acid–lipid conjugates for the programmable organisation of liposomal modules

Colloids with valence and specific directional bonding

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