Fabrication and modification of dual-faced nano-mushrooms for tri-functional cell theranostics: SERS/fluorescence signaling, protein targeting, and drug delivery

Hsieh, Hsin-Yi; Huang, Tsu-Wei; Xiao, Jian-Long; Yang, Ching‐Fen; Chang, Chien-Cheng; Chu, Cheng Ying; Lo, Leu‐Wei; Wang, Shenq-Hann; Wang, Pen-Cheng; Chieng, Ching-Chang; Lee, Chau‐Hwang; Tseng, Fan‐Gang

doi:10.1039/c2jm32967k

Cited by 17 publications

(7 citation statements)

References 43 publications

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“…To stain the cell nuclei and actin filaments, the cells were fixed with 4% paraformaldehyde in Dulbecco's phosphate buffered saline (DPBS) for 15 min, followed by permeabilization with 0.5% Triton X-100 in PBS for 10 min. Then, the samples were blocked in 1% BSA solution for 45 min at room temperature 53,54 and exposed to Alexa Fluor 488 phalloidin (A12379, Invitrogen) for 1 h at 37 °C, and 1 μg mL −1 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma) for 5 min. Fluorescent images for each sample were captured using an inverted fluorescence microscope (Olympus IX70) with ex/em at 490/515 nm, 535/617 nm, 488/520 nm, and 358/461 nm for calcein AM, propidium iodide, Alexa Fluor 488 phalloidin, and DAPI, respectively.…”

Section: Cell Viability Assaymentioning

confidence: 99%

Gradient static-strain stimulation in a microfluidic chip for 3D cellular alignment

et al. 2014

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Cell alignment is a critical factor to govern cellular behavior and function for various tissue engineering applications ranging from cardiac to neural regeneration. In addition to physical geometry, strain is a crucial parameter to manipulate cellular alignment for functional tissue formation. In this paper, we introduce a simple approach to generate a range of gradient static strains without external mechanical control for the stimulation of cellular behavior within 3D biomimetic hydrogel microenvironments. A glass-supported microfluidic chip with a convex flexible polydimethylsiloxane (PDMS) membrane on the top was employed for loading the cells suspended in a prepolymer solution. Following UV crosslinking through a photomask with a concentric circular pattern, the cell-laden hydrogels were formed in a height gradient from the center (maximum) to the boundary (minimum). When the convex PDMS membrane retracted back to a flat surface, it applied compressive gradient forces on the cell-laden hydrogels. The concentric circular hydrogel patterns confined the direction of hydrogel elongation, and the compressive strain on the hydrogel therefore resulted in elongation stretch in the radial direction to guide cell alignment. NIH3T3 cells were cultured in the chip for 3 days with compressive strains that varied from ~65% (center) to ~15% (boundary) on hydrogels. We found that the hydrogel geometry dominated the cell alignment near the outside boundary, where cells aligned along the circular direction, and the compressive strain dominated the cell alignment near the center, where cells aligned radially. This study developed a new and simple approach to facilitate cellular alignment based on hydrogel geometry and strain stimulation for tissue engineering applications. This platform offers unique advantages and is significantly different than the existing approaches owing to the fact that gradient generation was accomplished in a miniature device without using an external mechanical source.

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Section: Cell Viability Assaymentioning

confidence: 99%

Gradient static-strain stimulation in a microfluidic chip for 3D cellular alignment

et al. 2014

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“…The free polystyrene hemisphere was modified with anti-CD44 to target overexpressed CD44 glycoproteins in cancer cells. The JPs exhibited 12-fold cancer targeting ability and 10-fold enhancement in SERS signal compared with smooth gold nanoparticles [ 52 ]. Au–Ag-polyaniline (PA) JPs have been used as SERS tags for diagnosis of rheumatoid arthritis (RA) (Fig.…”

Section: Sensing and Biosensing With Jps And Jmsmentioning

confidence: 99%

“…Regarding cellular biosensing applications, the development of antibody-modified JPs for targeting and SERS sensing of tumor cells [ 51 , 52 ] and JPs which combine sensing and barcoding functions to enable multiplexed bioanalysis [ 72 – 78 ] should be mentioned.…”

Section: Sensing and Biosensing With Jps And Jmsmentioning

confidence: 99%

Janus particles and motors: unrivaled devices for mastering (bio)sensing

et al. 2021

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“…Using a similar strategy, Hsieh et al later incorporated four functions into a single Janus particle: targeting, SERS and fluorescence imaging, and drug delivery. 142 Their Janus particles had a fluorescently labeled polystyrene hemisphere that was conjugated with anti-CD44 antibodies, and a gold hemisphere that was functionalized with a disulfide linker (biotin-SS-NHS-sulfo) that can be cleaved in the cytoplasm for drug delivery.…”

Section: Biosensing Enabled By the Spatial Segregation Of Janus Parti...mentioning

confidence: 99%

Janus particles for biological imaging and sensing

Sanchez

Gao

et al. 2016

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Janus particles, named after the two-faced Roman god Janus, have different surface makeups, structures or compartments on two sides. This review highlights recent advances in employing Janus particles as novel analytical tools for live cell imaging and biosensing. Unlike conventional particles used in analytical science, two-faced Janus particles provide asymmetry and directionality, and can combine different or even incompatible properties within a single particle. The broken symmetry enables imaging and quantification of rotational dynamics, revealing information beyond what traditional measurements offer. The spatial segregation of molecules on the surface of a single particle also allows analytical functions that would otherwise interfere with each other to be decoupled, opening up opportunities for novel multimodal analytical methods. We summarize here the development of Janus particles, a few general methods for their fabrication and, more importantly, the emerging and novel applications of Janus particles as multi-functional imaging probes and sensors.

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Fabrication and modification of dual-faced nano-mushrooms for tri-functional cell theranostics: SERS/fluorescence signaling, protein targeting, and drug delivery

Cited by 17 publications

References 43 publications

Gradient static-strain stimulation in a microfluidic chip for 3D cellular alignment

Gradient static-strain stimulation in a microfluidic chip for 3D cellular alignment

Janus particles and motors: unrivaled devices for mastering (bio)sensing

Janus particles for biological imaging and sensing

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