Fabrication and in vitro biological properties of piezoelectric bioceramics for bone regeneration

Tang, Yufei; Wu, Cong; Wu, Zixiang; Hu, Long; Zhang, Wei; Zhao, Kang

doi:10.1038/srep43360

Cited by 123 publications

(110 citation statements)

References 53 publications

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“…The highest piezoelectric coefficients, which quantify the generation of charge per unit of mechanical load, are currently achieved by lead‐based piezoelectric ceramics; however, their high lead content renders them toxic (Shrout & Zhang, ). Among the lead‐free piezoelectric systems, barium titanate (BT)‐based materials are a promising class for bone replacement as indicated by cytotoxicity, cell viability, and proliferation studies (Ball, Mound, Nino, & Allen, ; Baxter, Bowen, Turner, & Dent, ; Park et al, ; Tang et al, ; Zhang, Chen, Zeng, Zhou, & Zhang, ). Particularly, the BT‐derivative (Ba,Ca)(Zr,Ti)O 3 (BCZT) is of interest because of exceptionally high piezoelectric values compared to other lead‐free piezoelectric materials available today (Liu & Ren, ).…”

Section: Introductionmentioning

confidence: 99%

Biocompatibility of (Ba,Ca)(Zr,Ti)O₃ piezoelectric ceramics for bone replacement materials

et al. 2019

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Total joint replacement implants are generally designed to physically mimic the biological environment to ensure compatibility with the host tissue. However, implant instability exposes patients to long recovery periods, high risk for revision surgeries, and high expenses. Introducing electrical stimulation to the implant site to accelerate healing is promising, but the cumbersome nature of wired devices is detrimental to the implant design. We propose a novel strategy to stimulate cells at the implant site by utilizing piezoelectric ceramics as electrical stimulation sources. The inherent ability of these materials to form electric surface potentials under mechanical load allows them to act as internal power sources. This characteristic is commonly exploited in non‐biomedical applications such as transducers or sensors. We investigate calcium/zirconium‐doped barium titanate (BCZT) ceramics in an in vitro environment to determine their potential as implant materials. BCZT exhibits low cytotoxicity with human osteoblast and endothelial cells as well as high piezoelectric responses. Microstructural adaptation was identified as a route for optimizing piezoelectric behavior. Our results show that BCZT is a promising system for biomedical applications. Its characteristic ability to autonomously generate electric surface potentials opens the possibility to functionalize existing bone replacement implant designs to improve implant ingrowth and long‐term stability.

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Section: Introductionmentioning

confidence: 99%

Biocompatibility of (Ba,Ca)(Zr,Ti)O₃ piezoelectric ceramics for bone replacement materials

et al. 2019

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“…Given this fact, piezoelectric polymers and bioceramics had been investigated as potential substrates to accelerate bone regeneration . For example, piezoelectric poly(vinylidene fluoride) (PVDF) and barium titanate (BaTiO 3 ) were found able to promote osteogenic differentiation of pluripotent stem cells . Zhang et al prepared a kind of PVDF/BaTiO 3 composite membrane to promote the regeneration of rat calvarial defect through sustainably maintained in vivo electric microenvironment .…”

Section: Introductionmentioning

confidence: 99%

“…21,22 For example, piezoelectric poly(vinylidene fluoride) (PVDF) and barium titanate (BaTiO 3 ) were found able to promote osteogenic differentiation of pluripotent stem cells. 23,24 Zhang et al prepared a kind of PVDF/BaTiO 3 composite membrane to promote the regeneration of rat calvarial defect through sustainably maintained in vivo electric microenvironment. 25 These observations spurred interests and successes in using external ES to enhance bone regeneration and healing.…”

Section: Introductionmentioning

confidence: 99%

Time‐dependent effect of electrical stimulation on osteogenic differentiation of bone mesenchymal stromal cells cultured on conductive nanofibers

Zhu

Wei

et al. 2017

J Biomedical Materials Res

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Bone tissue engineering using bone mesenchymal stromal cells (BMSCs) is a multidisciplinary strategy that requires biodegradable scaffold, cell, various promoting cues to work simultaneously. Electrical stimulation (ES) is known able to promote osteogenic differentiation of BMSCs, but it is interesting to know how can it play the strongest promotion effect. To strengthen local ES on BMSCs, parallel-aligned conductive nanofibers were electrospun from the mixtures of poly(L-lactide) (PLLA) and multi-walled carbon nanotubes (MWCNTs), and used for cell culture. Osteogenic differentiation of BMSCs was conducted by applying ES (direct current, 1.5 V, 1.5 h/day) perpendicular to the fiber direction during the day 1-7, day 8-14, or day 15-21 period of the osteoinductive culture. In comparison with ES-free groups, bone-related markers and genes were found significantly up-regulated when ES was applied on BMSCs growing on nanofibers having higher conductivity. When the ES was applied at the earlier stage of osteoinductive culture, the promotion effect on osteogenic differentiation would be stronger. In the presence of a BMP blocker, the down-regulated expressions of bone-related genes were able to be slightly recovered by ES, especially when the ES was applied at the beginning of osteoinductive culture (i.e. day 1-7). The promotion effect generated by ES in the early stage was found sustainable to later stages of differentiation, while those ES applied at later stages of differentiation should have missed the optimal point. In other words, later ES was not so necessary in inducing the osteogenic differentiation of BMSCs. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3369-3383, 2017.

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“…PLLA offers adjustable physical and mechanical properties and exhibits piezoelectricity (electrical charges generated by a mechanical force and vice versa) at a level comparable with collagen, the natural bone polymer . Indeed, it is known that surface charges can be used to modify cell behavior, and piezoelectricity has been explored in tissue repair applications . We have ourselves shown that protein adsorption is favored in poled PLLA areas, poling at temperatures higher than the polymer glass transition (T g ) producing polarization stable for up to 10 days, thus allowing for protein adhesion and cell proliferation in tissue growth strategies .…”

Section: Introductionmentioning

confidence: 94%

NMR metabolomics to study the metabolic response of human osteoblasts to non‐poled and poled poly (L‐lactic) acid

Araújo

Carneiro

Marinho

et al. 2019

Magnetic Reson in Chemistry

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Untargeted nuclear magnetic resonance (NMR) metabolomics was employed, for the first time to our knowledge, to characterize the metabolome of human osteoblast (HOb) cells and extracts in the presence of non‐poled or negatively poled poly‐L‐lactic acid (PLLA). The metabolic response of these cells to this polymer, extensively used in bone regeneration strategies, may potentially translate into useful markers indicative of in vivo biomaterial performance. We present preliminary results of multivariate and univariate analysis of NMR spectra, which have shown the complementarity of lysed cells and extracts in terms of information on cell metabolome, and unveil that, irrespective of poling state, PLLA‐grown cells seem to experience enhanced oxidative stress and activated energy metabolism, at the cost of storage lipids and glucose. Possible changes in protein and nucleic acid metabolisms were also suggested, as well as enhanced membrane biosynthesis. Therefore, the presence of PLLA seems to trigger cell catabolism and anti‐oxidative protective mechanisms in HOb cells, while directing them towards cellular growth. This was not sufficient, however, to lead to a visible cell proliferation enhancement in the presence of PLLA, although a qualitative tendency for negatively poled PLLA to be more effective in sustaining cell growth than non‐poled PLLA was suggested. These preliminary results indicate the potential of NMR metabolomics in enlightening cell metabolism in response to biomaterials and their properties, justifying further studies of the fine effects of poled PLLA on these and other cells of significance in tissue regeneration strategies.

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Fabrication and in vitro biological properties of piezoelectric bioceramics for bone regeneration

Cited by 123 publications

References 53 publications

Biocompatibility of (Ba,Ca)(Zr,Ti)O₃ piezoelectric ceramics for bone replacement materials

Biocompatibility of (Ba,Ca)(Zr,Ti)O₃ piezoelectric ceramics for bone replacement materials

Time‐dependent effect of electrical stimulation on osteogenic differentiation of bone mesenchymal stromal cells cultured on conductive nanofibers

NMR metabolomics to study the metabolic response of human osteoblasts to non‐poled and poled poly (L‐lactic) acid

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Fabrication and in vitro biological properties of piezoelectric bioceramics for bone regeneration

Cited by 123 publications

References 53 publications

Biocompatibility of (Ba,Ca)(Zr,Ti)O3 piezoelectric ceramics for bone replacement materials

Biocompatibility of (Ba,Ca)(Zr,Ti)O3 piezoelectric ceramics for bone replacement materials

Time‐dependent effect of electrical stimulation on osteogenic differentiation of bone mesenchymal stromal cells cultured on conductive nanofibers

NMR metabolomics to study the metabolic response of human osteoblasts to non‐poled and poled poly (L‐lactic) acid

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Biocompatibility of (Ba,Ca)(Zr,Ti)O₃ piezoelectric ceramics for bone replacement materials

Biocompatibility of (Ba,Ca)(Zr,Ti)O₃ piezoelectric ceramics for bone replacement materials