Fabrication and in vitro biocompatibility of biomorphic PLGA/nHA composite scaffolds for bone tissue engineering

Qian, Junmin; Xu, Weiqing; Yong, Xueqing; Jin, Xinxia; Zhang, Wei

doi:10.1016/j.msec.2013.11.047

Cited by 69 publications

(39 citation statements)

References 46 publications

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“…Nanotechnology has made it possible to create structures within the same size as those that constitute naturally occurring bone, opening a new era for TERM. Hence, nanoparticles (NPs) can be used to modify scaffolds properties, leading to enhanced characteristics such as superior mechanical properties and osteointegration, osteoconduction, and osteoinduction . Moreover, NPs can be applied to deliver drugs in a controlled and dependent manner, either systemically or locally .…”

Section: Introductionmentioning

confidence: 99%

“…Hence, nanoparticles (NPs) can be used to modify scaffolds properties, leading to enhanced characteristics such as superior mechanical properties [2][3][4][5] and osteointegration, osteoconduction, and osteoinduction. 2,[6][7][8][9] Moreover, NPs can be applied to deliver drugs in a controlled and dependent manner, either systemically or locally. [10][11][12][13][14][15][16][17][18] In another approach, NPs can be used to label cells, namely stem cells, enabling the continuous cell tracking and monitoring of its fate.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nanoparticles for bone tissue engineering

Vieira¹,

Vial²,

Reis³

et al. 2017

Biotechnology Progress

165

137

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Tissue engineering (TE) envisions the creation of functional substitutes for damaged tissues through integrated solutions, where medical, biological, and engineering principles are combined. Bone regeneration is one of the areas in which designing a model that mimics all tissue properties is still a challenge. The hierarchical structure and high vascularization of bone hampers a TE approach, especially in large bone defects. Nanotechnology can open up a new era for TE, allowing the creation of nanostructures that are comparable in size to those appearing in natural bone. Therefore, nanoengineered systems are now able to more closely mimic the structures observed in naturally occurring systems, and it is also possible to combine several approaches - such as drug delivery and cell labeling - within a single system. This review aims to cover the most recent developments on the use of different nanoparticles for bone TE, with emphasis on their application for scaffolds improvement; drug and gene delivery carriers, and labeling techniques. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:590-611, 2017.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nanoparticles for bone tissue engineering

Vieira¹,

Vial²,

Reis³

et al. 2017

Biotechnology Progress

165

137

View full text Add to dashboard Cite

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“…Porous hydroxyapatite (pHAP) is often used for the ceramic phase, is known for its excellent osteoconductivity and even osteoinductivity . For polymer phase, poly(lactic‐co‐glycolic) acid (PLGA) is approved as biodegradable, biocompatible, and noncytotoxic polymer . Furthermore, polysaccharide‐based structural polymers may improve chemical and functional properties of different ceramic scaffold structures, which is important for cell adhesion and growth.…”

Section: Introductionmentioning

confidence: 99%

“…14 For polymer phase, poly(lactic-co-glycolic) acid (PLGA) is approved as biodegradable, biocompatible, and noncytotoxic polymer. 15,16 Furthermore, polysaccharidebased structural polymers may improve chemical and functional properties of different ceramic scaffold structures, which is important for cell adhesion and growth. Natural polysaccharide alginate has already shown good characteristics as a polymer for bone tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells

Karadžić

Vučić

Jokanovic

et al. 2014

J. Biomed. Mater. Res.

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The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by "outgrowth" method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiation and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro.

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“…According to literature, nHA can enhance the attachment, proliferation, and differentiation of bone forming cells, as well as in vivo bone generation. 27,28 Such nHA incorporation was shown to enhance the expression of osteogenic related genes in human mesenchymal stem cells, such as ALP, osteocalcin, and bone sialoprotein, and promote ECM mineralization. In dental regeneration, a previous study also indicated that nHA had an effect on cell proliferation, differentiation, and ECM production of porcine tooth bud cells.…”

Section: Discussionmentioning

confidence: 99%

Influence of highly porous electrospun PLGA/PCL/nHA fibrous scaffolds on the differentiation of tooth bud cellsin vitro

Cai

Hoopen

Zhang

et al. 2017

J Biomedical Materials Res

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In this study, we investigated the use of three-dimensional electrospun poly(lactic-co-glycolic acid)/poly(ε-caprolactone) (PLGA/PCL) scaffolds seeded and cultured with postnatal dental cells, for improved dental tissue regeneration. Wet-electrospinning combined with ultrasonic treatment was studied as a method to enhance scaffold porosity and to promote cell-cell interactions. We also investigated whether nano-hydroxyapatite (nHA) incorporation could enhance dental cell differentiation. All scaffolds were seeded with human tooth pulp-derived dental mesenchymal (hDM) cells, or a combination of hDM and pig dental epithelial (pDE) cells, cultured for up to 28 days. Developmentally staged samples were assessed using scanning electron microscopy, histological, immunohistochemical, DNA and alkaline phosphatase activity assays, and quantitative-PCR for ameloblastic, odontoblastic, and osteogenic related gene expression. Results showed that electrospun scaffolds exhibited sufficient porosity to support robust cell ingrowth. Additional ultrasonic treatment led to a less homogeneous scaffold porosity, resulting in evident cell clustering and enhanced hDM-pDE cell-cell interactions. Finally, nHA incorporation was found to enhance dental cell differentiation. However, it also resulted in smaller fibre diameter and reduced scaffold porosity, and inhibited cell ingrowth and proliferation. In conclusion, ultrasonically treated wet-electrospun PLGA/PCL scaffolds are a suitable material for dental tissue engineering, and support future in vivo evaluations of this model.

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Fabrication and in vitro biocompatibility of biomorphic PLGA/nHA composite scaffolds for bone tissue engineering

Cited by 69 publications

References 46 publications

Nanoparticles for bone tissue engineering

Nanoparticles for bone tissue engineering

Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells

Influence of highly porous electrospun PLGA/PCL/nHA fibrous scaffolds on the differentiation of tooth bud cellsin vitro

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