Fabrication and Hemocompatibility Evaluation of a Robust Honeycomb Nanostructure on Medical Pure Titanium Surface

Abstract

Thrombosis induced by blood-contacting medical devices is still a major clinical problem, resulting in some serious complications such as infarction, irreversible tissue damage, and even death. Therefore, seeking an effective and safe surface modification approach to improve the hemocompatibility of the material is still urgent. In this research, a novel and facile approach was proposed to fabricate a robust honeycomb nanostructure on medical pure titanium surface by two-step anodic oxidation, which effectively enhanced the physicochemical performance and hemocompatibility of the material. Especially, the honeycomb nanostructure that underwent annealing treatment at 500 °C (HN-Ti-500 °C) presented significant performance to suppress the coagulation cascade in the in vitro tests, the reason mainly ascribed to an overall repulsive interaction between the protein molecule related to thrombosis and material surface based on an extended Derjaguin−Landau− Verwey−Overbeek (XDLVO) theory analysis. Furthermore, a vessel stent fabricated by HN-Ti-500 °C was implanted into the left carotid artery of rabbits for 1 month. The antithrombotic mechanism and biocompatibility of the modified surface were further verified. The results presented that no thrombus generated and adhered onto the inner surface of the modified stent, and no obvious disorder hyperplasia and inflammation were observed in the intima tissue of the vessel at the implantation site, which indicated that the modified surface could effectively decrease the risk of in-stent restenosis and thrombosis. This work offers a promising strategy for surface modification of bloodcontacting medical titanium material to address the clinical complications associated with restenosis and thrombosis.

Silicon nanowire array overcomes chemotherapeutic resistance by inducing the differentiation of breast cancer stem cells

“…The biocompatibility of nanomaterials is considered to be related to the surface structure and is important for their application. [41][42][43] In this WAs was higher than that on wafer (Figure 3E). The cell density of Si wafer was lower than that on culture plate and SiNWAs (Figure 3F).…”

“…The biocompatibility of nanomaterials is considered to be related to the surface structure and is important for their application 41–43 . In this work, the cytotoxicity effects of SiNWA and Si wafer on BCSCs were qualitatively and quantitatively evaluated by live/dead cellular staining and MTT assay, respectively.…”

A Highly Selective Implantable Electrochemical Fiber Sensor for Real-Time Monitoring of Blood Homovanillic Acid

“…To evaluate the biocompatibility of the HVA fiber sensor, cytotoxicity was first studied, and no significant differences in cell morphology, cell viability, and cell proliferation were observed compared to the control group after coculturing the HVA sensor with rat fibroblasts L929 and human umbilical vein endothelial cells (HUVEC) for 2 days (SI, Figure S20). Blood compatibility was then validated by plate adhesion, hemolysis, dynamic coagulation, and fibrin deposition tests. , The platelet adhesion behavior of the HVA sensor was not significantly different from that of the medical implantable titanium (Ti) wire (SI, Figure S21). The hemolysis rate of the sensor was only 0.57% (SI, Figure S22), and the dynamic coagulation time was consistent with that of the blank control group and Ti wire group (SI, Figure S23).…”

“…Blood compatibility was then validated by plate adhesion, hemolysis, dynamic coagulation, and fibrin deposition tests. 38,39 The platelet adhesion behavior of the HVA sensor was not significantly different from that of the medical implantable titanium (Ti) wire (SI, Figure S21). The hemolysis rate of the sensor was only 0.57% (SI, Figure S22), and the dynamic coagulation time was consistent with that of the blank control group and Ti wire group (SI, Figure S23).…”

Computational simulation of stent thrombosis induced by various degrees of stent malapposition

“…Most studies have focused on the effect of SM on flow stagnation as opposed to the biomechanical association between blood flow and thrombus growth. As ST is correlated with platelet activation and coagulation, it is necessary to include blood substances in fluid simulations ( Zhang et al, 2022 ).…”