Fabrication and evaluation of chitosan/gelatin/PVA hydrogel incorporating honey for wound healing applications: An in vitro, in vivo study

Shamloo, Amir; Aghababaie, Zahra; Afjoul, Homa; Jami, Mehrzad; Bidgoli, Mina Razaghzadeh; Vossoughi, Manouchehr; Ramazani, Ahmad; Kamyab‐Hesari, Kambiz

doi:10.1016/j.ijpharm.2020.120068

Cited by 116 publications

(61 citation statements)

References 41 publications

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“…Skin[ 50 , 51 ] and muscle[ 52 , 53 ] bioprinting have been addressed in 4% and 3% of the total manuscripts, respectively. These tissues and organs are relatively simple in architecture and composition, and yet they present substantial challenges to the current state-of-the-art bioprinting platforms.…”

Section: Applicationsmentioning

confidence: 99%

Bioinks for 3D Bioprinting: A Scientometric Analysis of Two Decades of Progress

Pedroza-González¹,

Rodríguez-Salvador²,

Pérez-Benítez³

et al. 2024

IJB

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This scientometric analysis of 393 original papers published from January 2000 to June 2019 describes the development and use of bioinks for 3D bioprinting. The main trends for bioink applications and the primary considerations guiding the selection and design of current bioink components (i.e., cell types, hydrogels, and additives) were reviewed. The cost, availability, practicality, and basic biological considerations (e.g., cytocompatibility and cell attachment) are the most popular parameters guiding bioink use and development. Today, extrusion bioprinting is the most widely used bioprinting technique. The most reported use of bioinks is the generic characterization of bioink formulations or bioprinting technologies (32%), followed by cartilage bioprinting applications (16%). Similarly, the cell-type choice is mostly generic, as cells are typically used as models to assess bioink formulations or new bioprinting methodologies rather than to fabricate specific tissues. The cell-binding motif arginine-glycine-aspartate is the most common bioink additive. Many articles reported the development of advanced functional bioinks for specific biomedical applications; however, most bioinks remain the basic compositions that meet the simple criteria: Manufacturability and essential biological performance. Alginate and gelatin methacryloyl are the most popular hydrogels that meet these criteria. Our analysis suggests that present-day bioinks still represent a stage of emergence of bioprinting technology.

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Section: Applicationsmentioning

confidence: 99%

Bioinks for 3D Bioprinting: A Scientometric Analysis of Two Decades of Progress

Pedroza-González¹,

Rodríguez-Salvador²,

Pérez-Benítez³

et al. 2024

IJB

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“…Rats were anesthetized using IP injection of ketamine hydrochloride (50 mg/kg body weight) and xylazine (5 mg/kg body weight) [38]. After removing the back hair of rats and disinfecting the shaved area, full-thickness round wounds with a diameter of 12 mm were developed by a sterile scalpel.…”

Section: Methodsmentioning

confidence: 99%

“…Scaffolds (n = 3) were immersed in 2 mL lysozyme solution in PBS pH 7.4 and incubated at 37 • C for 30 days. The lysozyme solution was refreshed every 2 days to maintain constant enzyme activity [38]. To examine the scaffold degradation rate, the lysozyme solution was removed at indicated time points, and samples were washed with sterile deionized water, dried and weighed.…”

Section: In Vitro Biodegradationmentioning

confidence: 99%

Glibenclamide Nanocrystal-Loaded Bioactive Polymeric Scaffolds for Skin Regeneration: In Vitro Characterization and Preclinical Evaluation

et al. 2021

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Skin restoration following full-thickness injury poses significant clinical challenges including inflammation and scarring. Medicated scaffolds formulated from natural bioactive polymers present an attractive platform for promoting wound healing. Glibenclamide was formulated in collagen/chitosan composite scaffolds to fulfill this aim. Glibenclamide was forged into nanocrystals with optimized colloidal properties (particle size of 352.2 nm, and polydispersity index of 0.29) using Kolliphor as a stabilizer to allow loading into the hydrophilic polymeric matrix. Scaffolds were prepared by the freeze drying method using different total polymer contents (3–6%) and collagen/chitosan ratios (0.25–2). A total polymer content of 3% at a collagen/chitosan ratio of 2:1 (SCGL3-2) was selected based on the results of in vitro characterization including the swelling index (1095.21), porosity (94.08%), mechanical strength, rate of degradation and in vitro drug release. SCGL3-2 was shown to be hemocompatible based on the results of protein binding, blood clotting and percentage hemolysis assays. In vitro cell culture studies on HSF cells demonstrated the biocompatibility of nanocrystals and SCGL3-2. In vivo studies on a rat model of a full-thickness wound presented rapid closure with enhanced histological and immunohistochemical parameters, revealing the success of the scaffold in reducing inflammation and promoting wound healing without scar formation. Hence, SCGL3-2 could be considered a potential dermal substitute for skin regeneration.

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“…In most previous studies, honey's wound healing effects have been emphasized more than their structural properties. 57 They have used honey as a non-combustible substance in dressing wounds. In contrast, according to this study's outcomes, hydrogel containing Aloe vera (Al-Chi-Alv) has desirable structural properties, including pore size, swelling, degradation, stability, and permeability.…”

Section: Discussionmentioning

confidence: 99%

Fabrication and characterization of alginate/chitosan hydrogel combined with honey and aloe vera for wound dressing applications

Saberian

Seyedjafari

Zargar

et al. 2021

J of Applied Polymer Sci

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Hydrogels can be one of the best polymeric wound dressings due to the desirable properties of wound healing. In this study, emphasizing the use of natural biomaterials such as Aloe vera and honey in the structure of cross-linked polymers, a novel hydrogel was produced that might be applied to healing wounds.In the beginning, four hydrogel groups were made from a combination of Sodium Alginate and Chitosan with Aloe vera extract and honey in optimum concentrations. Then the structure of those was evaluated by SEM and FTIR. After confirming hydrogels' structural properties, their physical properties, including swelling, porosity, density, mass loss, stability, and WVTR, were examined. Besides, the hydrogel biocompatibility was assessed by analyzing the cell viability and hemolytic activity. Adhesion of the cells to the hydrogel was also observed by SEM imaging. The results showed that the designed hydrogel has a porous structure with interconnected cavities, which their size can provide suitable conditions for cell adhesion, migration, and proliferation. Also, their physical and structural properties can be a strong suit to wound healing. Although honey's application can weaken the hydrogel structure, honey has beneficial properties due to its complex biomolecules. In contrast, Aloe vera in hydrogel generally improved the hydrogel's specificity for wound healing. According to the results of this study, taking advantage of hydrogels containing honey and Aloe vera based on alginate and chitosan polymers led to the formation of an acceptable structure and biocompatibility that can be used in future researches to repair tissues, especially wounds.

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Fabrication and evaluation of chitosan/gelatin/PVA hydrogel incorporating honey for wound healing applications: An in vitro, in vivo study

Cited by 116 publications

References 41 publications

Bioinks for 3D Bioprinting: A Scientometric Analysis of Two Decades of Progress

Bioinks for 3D Bioprinting: A Scientometric Analysis of Two Decades of Progress

Glibenclamide Nanocrystal-Loaded Bioactive Polymeric Scaffolds for Skin Regeneration: In Vitro Characterization and Preclinical Evaluation

Fabrication and characterization of alginate/chitosan hydrogel combined with honey and aloe vera for wound dressing applications

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