Fabrication and characterization of surgical sutures with propolis silver nano particles and analysis of its antimicrobial properties

Selvaraju, Gayathri Devi; Rekha, U. Vidhya; Sumathijones, C.; Cheema, Manraj Singh; Jayamani, D. Rachael; Dharani, R.; Sneha, S.; Yamuna, M.; Gayathiri, Ekambaram; Yadav, Sudesh

doi:10.1016/j.jksus.2022.102082

Cited by 9 publications

(14 citation statements)

References 22 publications

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“…coli and S. aureus . Syukri et al used the Eucalyptus camaldulensis leaf extract as a capping and reducing agent for AgNP synthesis and coated the silk suture.…”

Section: Resultsmentioning

confidence: 99%

“…The intensity changed depending on the incubation time. Therefore, EDS-based chemical composition was studied to specifically identify the presence of Ag, which showed a heterogeneous distribution of particles on the material surface. ,, The quantitative data are expressed in atomic % of Ag deposition as follows: compared with 48 h (24.8) and 96 h (7.3) of incubation time, the 24 h (32.2)-treated suture fragment showed more adsorption of NPs, which may be due to the supersaturation point or prolonged incubation time leading to desorption of particles under shaking conditions.…”

Section: Resultsmentioning

confidence: 99%

“…In recent studies published by Selvaraju et al, 20 nm AgNPs reduced by propolis have been used to coat catgut sutures and the antimicrobial effect of clinical pathogens. The results showed that NPs exhibited enhanced antimicrobial effects, thereby preventing SSI-based pathogens . Based on our search, there are very few investigations in this area; therefore, in this work, we developed highly cationic P-AgNPs using polyethylenimine (PEI) as both a reducing and stabilizing agent without using any extra reagents.…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial and Cytotoxic Effect of Positively Charged Nanosilver-Coated Silk Sutures

Monroy Caltzonci,

Rasu Chettiar,

Ibarra

et al. 2024

ACS Omega

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Sutures are a crucial component of surgical procedures, serving to close and stabilize wound margins to promote healing. However, microbial contamination of sutures can increase the risk of surgical site infections (SSI) due to colonization by pathogens. This study aimed to tackle SSI by synthesizing positively charged silver nanoparticles (P-AgNPs) and using them to produce antimicrobial sutures. The P-AgNPs were reduced and stabilized using polyethylenimine (PEI), a cationic branched polymer. The physiochemical characteristics of P-AgNPs were confirmed from the surface plasmon resonance (SPR) peak at 419 nm, spherical morphology with a particle size range of 8−10 nm, PEI functional groups on NPs, a hydrodynamic diameter of 12.3 ± 2.4 nm, and a zeta potential of 31.3 ± 6 mV. Subsequently, the surfaces of silk sutures were impregnated with P-AgNPs at different time intervals (24, 48, and 96 h) using an ex situ method. Scanning electron microscopy (SEM) and tensile strength studies were conducted to determine the coating and durability of the NP-coated sutures. The NPs were quantified on sutures using inductively coupled plasma optical emission spectrophotometry (ICP-OES), which was in the range of 1−5 μg. Primarily, antimicrobial activity was studied using three microorganisms (Candida albicans, Streptococcus mutans, and Staphylococcus aureus) for both P-AgNPs and suture-coated P-AgNPs using the agar diffusion method. The results showed that only the NPs and NP-coated sutures exhibited enhanced antimicrobial effects against bacteria and fungi. Finally, the cytotoxicity of the sutures was investigated using stem cells from the apical papilla (SCAPs) for 24 h, which exhibited more than 75% cell viability. Overall, the results indicate that NP-coated sutures can potentially be used as antimicrobial sutures to diminish or inhibit SSI in postoperative or general surgery patients.

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“…coli and S. aureus . Syukri et al used the Eucalyptus camaldulensis leaf extract as a capping and reducing agent for AgNP synthesis and coated the silk suture.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antimicrobial and Cytotoxic Effect of Positively Charged Nanosilver-Coated Silk Sutures

Monroy Caltzonci,

Rasu Chettiar,

Ibarra

et al. 2024

ACS Omega

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Section: Introductionmentioning

confidence: 99%

“…31,32 Therefore, there is a definite need for a new generation antimicrobial suture against multi drug resistance for overcoming SSIs and currently plenty of research studies were performed to obtain antimicrobial properties on surgical sutures. [33][34][35][36] Antimicrobial Peptides (AMPs) are the molecules of all multicellular organisms that take place in the innate immune system as the first line of defense with broad-spectrum antimicrobial effects against Gram-positive and Gramnegative bacteria, fungi, and viruses. 17,37,38 AMPs not only take a part in the innate immune system but also modulate the host's inflammatory response during infection.…”

Section: Introductionmentioning

confidence: 99%

Effect of antimicrobial peptide conjugated surgical sutures on multiple drug-resistant microorganisms

et al. 2022

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Surgical site infections are commonly encountered as a risk factor in clinics that increase the morbidity of a patient after a surgical operation. Surgical sutures are one of the leading factor for the formation of surgical site infections that induce bacterial colonization by their broad surface area. Current strategies to overcome with surgical site infections consist utilization of antibiotic agent coatings such as triclosan. However, the significant increase in antibiotic resistance majorly decreases their efficiency against recalcitrant pathogens such as; Pseudomonas aeruginosa and Staphylococcus aureus. Therefore, the development of a multi drug-resistant antimicrobial suture without any cytotoxic effect to combat surgical site infections is vital. Antimicrobial peptides are the first defense line which has a broad range of spectrum against Gram-positive, and Gram-negative bacteria and even viruses. In addition, antimicrobial peptides have a rapid killing mechanism which is enhanced by membrane disruption and inhibition of functional proteins in pathogens without the development of antimicrobial resistance. In the scope of the current study, the antimicrobial effect of antimicrobial peptide conjugated poly (glycolic acid-co-caprolactone) (PGCL) sutures were investigated against P. aeruginosa and methicillin-resistant S. aureus (MRSA) strains by using antimicrobial peptide sequences of KRFRIRVRV-NH2, RWRWRWRW-NH2 and their dual combination (1:1). In addition, in vitro wound scratch assays were performed to evaluate the effect of antimicrobial peptide conjugated sutures on keratinocyte cell lines. Our results indicated that antimicrobial peptide modified sutures could be a potential novel medical device to overcome surgical site infections by the superior acceleration of wound healing.

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Advances, challenges, and prospects for surgical suture materials

Meng

Chen

et al. 2023

Acta Biomaterialia

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Fabrication and characterization of surgical sutures with propolis silver nano particles and analysis of its antimicrobial properties

Cited by 9 publications

References 22 publications

Antimicrobial and Cytotoxic Effect of Positively Charged Nanosilver-Coated Silk Sutures

Antimicrobial and Cytotoxic Effect of Positively Charged Nanosilver-Coated Silk Sutures

Effect of antimicrobial peptide conjugated surgical sutures on multiple drug-resistant microorganisms

Advances, challenges, and prospects for surgical suture materials

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