Fabrication and characterization of six electrospun poly(α-hydroxy ester)-based fibrous scaffolds for tissue engineering applications

Li, Wan‐Ju; Cooper, James A.; Mauck, Robert L.; Tuan, Rocky S.

doi:10.1016/j.actbio.2006.02.005

Cited by 468 publications

(364 citation statements)

References 22 publications

(28 reference statements)

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“…However, glutaraldehyde-treated materials can be cytotoxic [44]. As previously shown, pure PCL-based fibrous scaffolds were hydrolysis-resistant for culture periods over 40 days [45]. In order to avoid chemical cross-linking of electrospun scaffolds, PCL was added to the mixture of collagen and elastin as well as gelatin during scaffold fabrication.…”

Section: Discussionmentioning

confidence: 99%

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

et al. 2008

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Electrospinning using natural proteins or synthetic polymers is a promising technique for the fabrication of fibrous scaffolds for various tissue engineering applications. However, one limitation of scaffolds electrospun from natural proteins is the need to cross-link with glutaraldehyde for stability, which has been postulated to lead to many complications in vivo including graft failure. In this study, we determined the characteristics of hybrid scaffolds composed of natural proteins including collagen and elastin, as well as gelatin, and the synthetic polymer poly(ε-caprolactone) (PCL), so to avoid chemical cross-linking. Fiber size increased proportionally with increasing protein and polymer concentrations, whereas pore size decreased. Electrospun gelatin/PCL scaffolds showed a higher tensile strength when compared to collagen/elastin/PCL constructs. To determine the effects of pore size on cell attachment and migration, both hybrid scaffolds were seeded with adipose-derived stem cells. Scanning electron microscopy and nuclei staining of cell-seeded scaffolds demonstrated complete cell attachment to the surfaces of both hybrid scaffolds, although cell migration into the scaffold was predominantly seen in the gelatin/PCL hybrid. The combination of natural proteins and synthetic polymers to create electrospun fibrous structures resulted in scaffolds with favorable mechanical and biological properties.

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Section: Discussionmentioning

confidence: 99%

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

et al. 2008

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“…Among the various types of biomaterials being developed, electrospun materials composed of ultra fine fibers are preferred for medical applications. Electrospun structures closely mimic the three-dimensional (3D) fibrous network found in the natural extracellular matrix (ECM) [6][7][8][9]. Electrospun materials also have a high surface to volume ratio that facilitates cell attachment and drug sorption [6,10] and contain a large volume of interconnected pores, which assist in the transportation of oxygen, nutrients and the metabolic waste of cells and cell migration and communication [8].…”

Section: Introductionmentioning

confidence: 98%

Cytocompatible cross-linking of electrospun zein fibers for the development of water-stable tissue engineering scaffolds

2010

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“…For such applications PLA e PLAGA copolymers with different compositions have been successfully foamed either as plain materials [117,125,129] or in combination with inorganic substances [87,130]. Moreover, scCO 2 foaming is a powerful technology for the production of scaffolds loaded with biomolecules such as growth factors [131][132][133][134][135] and DNA [134,135] that, once released, have been shown to maintain their activity. …”

Section: Gas Foamingmentioning

confidence: 99%

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Gualandi

2011

Springer Theses

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and some lactide copolymers) and of non-commercial polyesters (i.e. poly-ω-pentadecalactone and some of its copolymers) were explored and discussed.Two techniques were employed to fabricate scaffolds: supercritical carbon dioxide (scCO 2 ) foaming and electrospinning (ES). The former is a powerful technology that enables to produce 3D microporous foams by avoiding the use of solvents that can be toxic to mammalian cells. The scCO 2 process, which is II commonly applied to amorphous polymers, was successfully modified to foam a highly crystalline poly(ω-pentadecalactone-co-ε-caprolactone) copolymer and the effect of process parameters on scaffold morphology and thermo-mechanical properties was investigated.In the course of the present research activity, sub-micrometric fibrous nonwoven meshes were produced using ES technology. Electrospun materials are considered highly promising scaffolds because they resemble the 3D organization of native extra cellular matrix. A careful control of process parameters allowed to fabricate defect-free fibres with diameters ranging from hundreds of nanometers to several microns, having either smooth or porous surface. Moreover, versatility of ES technology enabled to produce electrospun scaffolds from different polyesters as well as "composite" non-woven meshes by concomitantly electrospinning different fibres in terms of both fibre morphology and polymer material. The 3D-architecture of the electrospun scaffolds fabricated in this research was controlled in terms of mutual fibre orientation by properly modifying the instrumental apparatus. This aspect is particularly interesting since the micro/nano-architecture of the scaffold is known to affect cell behaviour.Since last generation scaffolds are expected to induce specific cell response, the present research activity also explored the possibility to produce electrospun scaffolds bioactive towards cells. Bio-functionalized substrates were obtained by loading polymer fibres with growth factors (i.e. biomolecules that elicit specific cell behaviour) and it was demonstrated that, despite the high voltages applied during electrospinning, the growth factor retains its biological activity once

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Fabrication and characterization of six electrospun poly(α-hydroxy ester)-based fibrous scaffolds for tissue engineering applications

Cited by 468 publications

References 22 publications

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

Cytocompatible cross-linking of electrospun zein fibers for the development of water-stable tissue engineering scaffolds

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

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