Fabrication and Characterization of Scaffolds of Poly(<i>ε</i>-caprolactone)/Biosilicate® Biocomposites Prepared by Generative Manufacturing Process

Cunha, Daniel Aparecido Lopes Vieira da; Neto, Paulo Inforçatti; Micocci, Kelli Cristina; Bellani, Caroline Faria; Selistre-de-Araújo, Heloísa Sobreiro; Silveira, Zilda de Castro; Branciforti, Márcia Cristina

doi:10.1155/2019/2131467

Cited by 10 publications

(11 citation statements)

References 46 publications

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“…84 da Cunha 2019 fabricated PCL-Biosilicate scaffold using extrusion printer, with 0°/90°pore sizes and pore interconnectivity, which led to 57% increase in the stiffness of scaffold, without increasing any toxicity. 85 González-Gil (2019) used a bone nonunion model in Sprague-Dawley rats in six groups: control, live bone allograft, rhBMP-2 in collagen; acellular PCL; PCL with periosteumderived MSCs, and PCL containing bone marrow-derived MSCs. Significant new bone formation was seen in LBA, CS BMP2 and PCL PMSCs groups at 8 weeks.…”

Section: Cellular Bioactivity On Pcl Scaffoldsmentioning

confidence: 99%

Polycaprolactone as biomaterial for bone scaffolds: Review of literature

Dwivedi

Kumar

Pandey

et al. 2020

Journal of Oral Biology and Craniofacial Research

424

290

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Bone tissue engineering using polymer based scaffolds have been studied a lot in last decades. Considering the qualities of all the polymers desired to be used as scaffolds, Polycaprolactone (PCL) polyester apart from being biocompatible and biodegradable qualifies to an appreciable level due its easy availability, cost efficacy and suitability for modification. Its adjustable physio-chemical state, biological properties and mechanical strength renders it to withstand physical, chemical and mechanical, insults without significant loss of its properties. This review aims to critically analyse the efficacy of PCL as a biomaterial for bone scaffolds. 1.1. Polymers as biomaterial for scaffolds The characteristics of a biomaterial that must be scrutinized thoroughly before considering it for bone tissue engineering applications includes chemical composition, biological and structural characteristics, degradation behavior and fabrication process. Polymeric scaffolds are of considerable interest due to their distinctive features, such

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Section: Cellular Bioactivity On Pcl Scaffoldsmentioning

confidence: 99%

Polycaprolactone as biomaterial for bone scaffolds: Review of literature

Dwivedi

Kumar

Pandey

et al. 2020

Journal of Oral Biology and Craniofacial Research

424

290

View full text Add to dashboard Cite

show abstract

“…However, it is necessary to modify the filament to produce scaffolds for bone tissue regeneration using this technique. Cunha et al [14] produced 3D scaffolds of PCL/Biosilicate ® and the observed improvement in the mechanical properties and the addition of Biosilicate ® provided a good environment for cell attachment and proliferation [14]. It is worth noticing that the printed scaffold used a specific printing setup where the two separated materials were feed in powder form which may lead to some drawbacks as poor filler dispersion.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Degradation During Melt Processing of PLA/Biosilicate® Composites

et al. 2019

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Poly (lactic acid) (PLA)/bioactive composites are emerging as new biomaterials since it is possible to combine stiffness, mechanical resistance, and bioactive character of the bioglasses with conformability and bioabsorption of the PLA. In this study, PLA/Biosilicate® composites were prepared using a melt-processing route. The processability and properties were evaluated aiming to produce composites with bioactive properties. Two different PLA (PLA 2003D and PLA 4043D) were tested with the addition of 1 wt. % of Biosilicate®. Both materials presented a huge reduction in melt viscosity after internal mixer processing. The degradation effects of the addition of Biosilicate® in the PLAs matrices were evaluated using zeta potential tests that showed a very high liberation of ions, which catalyzes PLA thermo-oxidative reactions. To understand the extension of degradation effects during the processing, the composites were characterized using thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), gel permeation chromatography (GPC), and rheological tests. GPC results showed that PLA with the lowest residual acid content (RAC), PLA 2003D, presented higher thermal stability, higher molecular weight, and viscosity baseline compared to PLA 4043D. The composites showed a significant decrease in molecular weight for both PLA with the addition of Biosilicate®. TGA results showed that Biosilicate® might have reduced the activation energy to initiate thermodegradation reactions in PLAs and it occasioned a reduction in the Tonset by almost 40 °C. The DSC results showed that severe matrix degradation and the presence of bioglass did not significantly affect glass transition temperature (Tg), melting temperature (Tm) and crystallinity of PLAs, but it influenced cold crystallization peak (Tcc). In this way, the type of PLA used influences the processability of this material, which can make the production of filaments of this material for 3D printing unfeasible.

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“…It can be obtained a filament that forms a 3D scaffold geometry laid up on a platform [25]. Few studies [26,27] report its efficiency in tissue engineering manufacture. Dávila et al [26] and Cunha et al [27] studied the fabrication of polymer-ceramic scaffolds and reported the formation of undesirable ceramic aggregates in the polymeric matrix, which can be improved by adjusting the operational parameters during the manufacturing process, or applying a pre-process.…”

Section: Introductionmentioning

confidence: 99%

Fabrication, morphological, mechanical and biological performance of 3D printed poly(ϵ-caprolactone)/bioglass composite scaffolds for bone tissue engineering applications

Barbosa

Dernowsek²,

Tobar

et al. 2022

Biomed. Mater.

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Several techniques, such as additive manufacturing, have been used for the manufacture of polymer-ceramic composite scaffolds for bone tissue engineering. A new extruder head recently developed for improving the manufacturing process is an experimental 3D printer Fab@CTI that enables the use of ceramic powders in the processing of composite materials or polymer blends. Still, the manufacturing process needs improvement to promotes the dispersion of ceramic particles in the polymer matrix. This article addresses the manufacture of scaffolds by 3D printing from mixtures of poly(ε-caprolactone) (PCL) and a glass powder of same composition of 45S5Bioglass®, labeled as synthesized bioglass (SBG), according to two different methods that investigated the efficiency of the new extruder head. The first one is a single extrusion process in a Fab@CTI 3D printer, and the other consists in the pre-processing of the PCL-SBG mixture in a mono-screw extruder with a Maddock® element, followed by direct extrusion in the experimental Fab@CTI 3D printer. The morphological characterization of the extruded samples by SEM showed an architecture of 0o/90o interconnected struts and suitable porosity for bone tissue engineering applications. Scaffolds fabricated by two methods shows compressive modulus ranging from 54.4 ± 14.2 MPa to 155.9 ± 20.4 MPa, results that are compatible to use in bone tissue engineering. Cytotoxicity assays showed non-toxic effects and viability for in vitro MG-63 cell proliferation. Alizarin Red staining test showed calcium deposition in all scaffolds, which suggests PCL/SBG composites promising candidates for use in bone tissue engineering. Results of cell morphology suggest more cell growth and adhesion for scaffolds fabricated using the pre-processing in a mono-screw extruder.

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Fabrication and Characterization of Scaffolds of Poly(ε-caprolactone)/Biosilicate® Biocomposites Prepared by Generative Manufacturing Process

Cited by 10 publications

References 46 publications

Polycaprolactone as biomaterial for bone scaffolds: Review of literature

Polycaprolactone as biomaterial for bone scaffolds: Review of literature

Analysis of the Degradation During Melt Processing of PLA/Biosilicate® Composites

Fabrication, morphological, mechanical and biological performance of 3D printed poly(ϵ-caprolactone)/bioglass composite scaffolds for bone tissue engineering applications

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