Fabrication and Characterization of Porous Flow-Assembled Chitosan Membranes in Microfluidics

Ly, Khanh L.; Luo, Xiaolong

doi:10.1007/978-3-030-75506-5_31

Cited by 2 publications

(2 citation statements)

References 23 publications

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“…During the fabrication of chitosan membranes, optical images were taken every 30 s, and the membrane thickness was measured with ImageJ 1.51j8 (NIH, Bethesda, MD, USA). The growth rate of chitosan membranes was determined by plotting the membrane thickness with respect to time [ 46 , 48 ], which revealed the correlation between membrane growth and fabrication parameters (voltage and pH of alginate).…”

Section: Methodsmentioning

confidence: 99%

Programmable Physical Properties of Freestanding Chitosan Membranes Electrofabricated in Microfluidics

Piao

Raub

et al. 2023

Membranes

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Microfluidic-integrated freestanding membranes with suitable biocompatibility and tunable physicochemical properties are in high demand for a wide range of life science and biological studies. However, there is a lack of facile and rapid methods to integrate such versatile membranes into microfluidics. A recently invented interfacial electrofabrication of chitosan membranes offers an in-situ membrane integration strategy that is flexible, controllable, simple, and biologically friendly. In this follow-up study, we explored the ability to program the physical properties of these chitosan membranes by varying the electrofabrication conditions (e.g., applied voltage and pH of alginate). We found a strong association between membrane growth rate, properties, and fabrication parameters: high electrical stimuli and pH of alginate resulted in high optical retardance and low permeability, and vice versa. This suggests that the molecular alignment and density of electrofabricated chitosan membranes could be actively tailored according to application needs. Lastly, we demonstrated that this interfacial electrofabrication could easily be expanded to produce chitosan membrane arrays with higher uniformity than the previously well-established flow assembly method. This study demonstrates the tunability of the electrofabricated membranes’ properties and functionality, thus expanding the utility of such membranes for broader applications in the future.

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Section: Methodsmentioning

confidence: 99%

Programmable Physical Properties of Freestanding Chitosan Membranes Electrofabricated in Microfluidics

Piao

Raub

et al. 2023

Membranes

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“…The mold for the microfluidic platform was manufactured on a 4 ′′ silicon wafer using conventional photolithography technique with negative photoresist SU-8 3035. The PDMS chip was constructed using soft-lithography by mixing Sylgard 184 and its curing agent (Ellsworth Adhesives, NY, USA) at a ratio of 10:1, degassed, poured on top of the patterned silicon mold, and cured at 65 • C for 4 h [38,39]. Next, the cured PDMS was peeled off from the mold and cut into a set of eight devices per chip.…”

Section: Microfluidic Platform Fabricationmentioning

confidence: 99%

Oral mucositis on a chip: modeling induction by chemo- and radiation treatments and recovery

Ly¹,

Luo²,

Raub³

2022

Biofabrication

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Oral mucositis (OM) is a debilitating complication affecting roughly 70% of head and neck cancer patients receiving chemotherapy and/or radiation treatment. No broadly effective preventative treatment for OM exists. Therefore, an in vitro model of cancer treatment-induced OM would aid studies into possible origins of the pathology and future drug targets to ameliorate it. In this study, we present a microfluidic oral mucosa triculture tissue construct consisting of a keratinocyte layer attached to a subepithelial fibroblast and endothelial cell-embedded collagen gel. To address the typically low stability of mucosal constructs in microfluidics, ruthenium-catalyzed photocrosslinking was implemented to strengthen the collagen gel and prevent the invasion of kerotinacytes, thus maintaining tissue construct geometry and oral mucosa barrier function for over 18 days of culture. Next, the OM chip was exposed to cisplatin (day 10) and damaging radiation (day 11, +/- cisplatin at day 10), mimicking damage from cancer therapy. Damage to and then recovery of the tissue layers and function were observed over days 11-18. Therefore, several important features of oral mucositis induction and resolution were modeled in microfluidic culture. The oral mucositis model on a chip allows for more sophisticated studies into mechanisms of OM and potential treatments.

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Fabrication and Characterization of Porous Flow-Assembled Chitosan Membranes in Microfluidics

Cited by 2 publications

References 23 publications

Programmable Physical Properties of Freestanding Chitosan Membranes Electrofabricated in Microfluidics

Programmable Physical Properties of Freestanding Chitosan Membranes Electrofabricated in Microfluidics

Oral mucositis on a chip: modeling induction by chemo- and radiation treatments and recovery

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