Fabrication and characterization of poly(lactic-co-glycolic acid)/polyvinyl alcohol blended hollow fibre membranes for tissue engineering applications

Meneghello, Giulia; Parker, Daniel J.; Ainsworth, Ben; Perera, Semali; Chaudhuri, Julian B.; Ellis, Marianne; Bank, Paul De

doi:10.1016/j.memsci.2009.07.034

Cited by 50 publications

(37 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is possible to make fibers by spinning from a variety of materials suitable for cell culture, both biodegradable and non-biodegradable, for example; polycaprolactone (PCL) 27 , poly-L-lactide acid (PLLA) 28 , poly(lactic-co-glycolic acid) (PLGA) 29 , polysulfone (PSU) 12 and polyetheretherketone (PEEK)…”

Section: Discussionmentioning

confidence: 99%

Hollow Fiber Bioreactors for <em>In Vivo</em>-like Mammalian Tissue Culture

Storm

Sorrell

Shipley

et al. 2016

JoVE

Self Cite

View full text Add to dashboard Cite

Tissue culture has been used for over 100 years to study cells and responses ex vivo. The convention of this technique is the growth of anchorage dependent cells on the 2-dimensional surface of tissue culture plastic. More recently, there is a growing body of data demonstrating more in vivo-like behaviors of cells grown in 3-dimensional culture systems. This manuscript describes in detail the set-up and operation of a hollow fiber bioreactor system for the in vivo-like culture of mammalian cells. The hollow fiber bioreactor system delivers media to the cells in a manner akin to the delivery of blood through the capillary networks in vivo. The system is designed to fit onto the shelf of a standard CO 2 incubator and is simple enough to be set-up by any competent cell biologist with a good understanding of aseptic technique. The systems utility is demonstrated by culturing the hepatocarcinoma cell line HepG2/C3A for 7 days. Further to this and in line with other published reports on the functionality of cells grown in 3-dimensional culture systems the cells are shown to possess increased albumin production (an important hepatic function) when compared to standard 2-dimensional tissue culture. Video LinkThe video component of this article can be found at

show abstract

Section: Discussionmentioning

confidence: 99%

Hollow Fiber Bioreactors for <em>In Vivo</em>-like Mammalian Tissue Culture

Storm

Sorrell

Shipley

et al. 2016

JoVE

Self Cite

View full text Add to dashboard Cite

show abstract

“…Melt moulding, solution casting, phase separation, solventcasting particulate-leaching, emulsion freeze drying, fibre meshes/fibre bonding, freeze drying, and gas foaming are some conventional scaffold fabrication techniques cited in the literature (Sachlos and Czernuszka 2003). Meneghello et al (2009) fabricated poly(lactic-coglycolic acid)/polyvinyl alcohol (PVA) blended hollow fibre membranes, where the results demonstrated that 5% (w/w) addition of PVA helped to better transport the cell culture medium (CCM) and its constituents. Bettahalli et al (2011a,b) developed polylactic acid (PLLA) hollow fibre membranes to test the delivery capability of these membranes to diffuse nutrients to the cells.…”

Section: Membrane Type Schematic Of Cross-section Referencesmentioning

confidence: 99%

Glucose diffusion in tissue engineering membranes and scaffolds

Suhaimi

Das

2016

Reviews in Chemical Engineering

View full text Add to dashboard Cite

Tissue engineering has evolved into an exciting area of research due to its potential in regenerative medicine. The shortage of organ donors as well as incompatibility between patient and donor pose an alarming concern. This has resulted in an interest in regenerative therapy where the importance of understanding the transport properties of critical nutrients such as glucose in numerous tissue engineering membranes and scaffolds is crucial. This is due to its dependency on successful tissue growth as a measure of potential cure for health issues that cannot be healed using traditional medical treatments. In this regard, the diffusion of glucose in membranes and scaffolds that act as templates to support cell growth must be well grasped. Keeping this in mind, this review paper aims to discuss the glucose diffusivity of these materials. The paper reviews four interconnected issues, namely, (i) the glucose diffusion in tissue engineering materials, (ii) porosity and tortuosity of these materials, (iii) the relationship between microstructure of the material and diffusion, and (iv) estimation of glucose diffusivities in liquids, which determine the effective diffusivities in the porous membranes or scaffolds. It is anticipated that the review paper would help improve the understanding of the transport properties of glucose in membranes and scaffolds used in tissue engineering applications.

show abstract

“…Additionally, in recent years, hollow-fiber membrane-based bioreactors have been reported as apt cell culture systems for therapeutic applications 9 , such as tissue engineering 21 or growing red blood cells 22 . Therefore, in this paper, the performance of hybridoma cells in a HFBR system was analyzed as a case study of cell culture in a membrane reactor.…”

Section: Jain and Kumarmentioning

confidence: 99%

“…[19][20][21][22]. The exponents of the inhibitory terms were assumed to be the same for glucose/glutamine consumption and lactate/ammonium production, both values being found to be quite similar (n LAC = 0.10 and n AM = 0.15, respectively).…”

Section: -Evolution Of Antibody Concentration (Samples Taken From Thementioning

confidence: 99%

Diffusion and Inhibition Processes in a Hollow-fiber Membrane Bioreactor for Hybridoma Culture. Development of a Mathematical Model

Legazpi

Laca

Collado

et al. 2016

Chem.Biochem.Eng.Q.

View full text Add to dashboard Cite

The performance of a hollow-fiber membrane bioreactor (HFBR) (molecular weight cut-off 30 kD, fiber surface area 2050 cm 2 ) containing a culture of hybridoma cells has been investigated. Experimental data were used as basis to develop a model of general application. Concentrations of fundamental nutrients (glucose and glutamine), inhibitory products (ammonium and lactate), and monoclonal antibodies (MAb) against bovine lactoferrin (IgG 1 ) were monitored over time. Exchange of nutrients and products occurred across the capillary surface, whereas cells and MAb remained in the extra-capillary space (ECS). A protein-free culture medium (Hybrimax) with and without antibiotics was used. In both cases, the final MAb concentration was the same; however, antibiotic presence slowed down the time to achieve this concentration. Diffusion assays have been carried out in order to support the development of a mathematical model that describes the performance of the HFBR, including mass transfer and reaction terms. Inhibition by ammonium and lactate has been considered in the kinetics, providing model results consistent with experimental data. Further research with other cell lines and/or culture media will allow to broaden the field of application of this model for general use in HFBR systems.

show abstract

Fabrication and characterization of poly(lactic-co-glycolic acid)/polyvinyl alcohol blended hollow fibre membranes for tissue engineering applications

Cited by 50 publications

References 50 publications

Hollow Fiber Bioreactors for <em>In Vivo</em>-like Mammalian Tissue Culture

Hollow Fiber Bioreactors for <em>In Vivo</em>-like Mammalian Tissue Culture

Glucose diffusion in tissue engineering membranes and scaffolds

Diffusion and Inhibition Processes in a Hollow-fiber Membrane Bioreactor for Hybridoma Culture. Development of a Mathematical Model

Contact Info

Product

Resources

About