Fabrication and characterization of egg white cryogel scaffold for three-dimensional (3D) cell culture

Balaji, Perumalsamy; Anbazhagan, Murugadas; Sellathamby, Shanmugaapriya; Akbarsha, Mohammad Abdulkader

doi:10.1016/j.bcab.2018.12.019

Cited by 11 publications

(14 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to these spectra, it is seen that the EW retained its functional groups after crosslinking (Balaji et al, 2019).…”

Section: Resultsmentioning

confidence: 97%

“…Chicken eggs (Local market), methylene Blue (MB, Merck), glutaraldehyde solution 25% (GA, Merck), hydrochloric acid (HCl, Merck), sodium hydroxide (NaOH, Merck) and n-hexane (Lab-scan) were used without further purification. EW cryogel was fabricated using glutaraldehyde as a crosslinker (Balaji et al, 2019). Firstly, the EW was carefully separated from the yolk in Figure 1.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Egg White Cryogel for Removal of Methylene Blue from Aqueous Solution

Gulyuz

2021

International Journal of Environment and Geoinformatics

View full text Add to dashboard Cite

Macroporous egg white (EW) cryogel synthesized by cryogelation at sub-zero temperature (-18 o C) with crosslinker glutaraldehyde (GA) was tested in the adsorption of methylene blue (MB) as a model pollutant from aqueous solution. The characterization of obtained cryogel was carried out using Fourier-transform infrared spectroscopy and scanning electron microscopy. The effects of initial dye concentration, pH and contact time parameters on the adsorption were systematically evaluated. The pseudo-first order and pseudo-second order models were used to predict the adsorption kinetics. The adsorption followed the pseudo-second order model. Two isotherm models called Langmuir and Freundlich were applied on the experimental data to predict the maximum adsorption capacity and explain the process of adsorption. Langmuir isotherm model provided the best fit with maximum adsorption capacity of 56.18 mg MB/g cryogel. The dye adsorbed EW cryogel was easily regenerated and used several times with no noticeable reduction of capacity. The electrostatic attraction was the main adsorption mechanism of MB on the cryogel, especially at slightly basic pH. Its easy preparation, low-cost and good adsorption property make the EW cryogel a promising adsorbent for environmental applications.

show abstract

“…According to these spectra, it is seen that the EW retained its functional groups after crosslinking (Balaji et al, 2019).…”

Section: Resultsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Egg White Cryogel for Removal of Methylene Blue from Aqueous Solution

Gulyuz

2021

International Journal of Environment and Geoinformatics

View full text Add to dashboard Cite

show abstract

“…Balaji et al developed an egg white 3D cell culture scaffold system crosslinked with glutaraldehyde. This kind of egg white scaffold with an interconnected macroporous structure showed great advantages in nutrition transportation, enhancing cell signal transmission and proliferation 65 . With egg whites, gelatin, corn starch, and sucrose as raw materials, 3D printing technology was successfully used to print complex 3D geometries.…”

Section: Application To Biomaterialsmentioning

confidence: 99%

“…This kind of egg white scaffold with an interconnected macroporous structure showed great advantages in nutrition transportation, enhancing cell signal transmission and proliferation. 65 With egg whites, gelatin, corn starch, and sucrose as raw materials, 3D…”

Section: D Cell Culture Systemmentioning

confidence: 99%

An insight on egg white: From most common functional food to biomaterial application

Dong

Zhang

2020

J Biomed Mater Res

View full text Add to dashboard Cite

Natural egg white tis widely used as an ingredient in nutritional foods and for food processing. Due to its characteristic foaming, emulsification, adhesion, and gelation, and its heat setting, biocompatibility, and low cost, research into the application and development of egg white in biomaterials, especially medical biomaterials, have been receiving attention. The composition and characteristics of egg white protein, and the physical mixing and chemically cross‐linking of egg white with other materials used to make degradable packaging films, bioceramics, bioplastics, biomimetic films, hydrogels, 3D scaffolds, bone regeneration, biopatterning, biosensors, and so forth, are reviewed in detail in this report. The novel egg white‐based biomaterials in various forms and applications could be constructed mostly through physical treatments such as ultrasonic wave, ultraviolet, laser and other radiation or high‐temperature calcination. Furthermore, the application and prospects for the use of egg white in biomaterials is also discussed.

show abstract

“… 17 − 19 We conducted a preliminary study of an EW cryogel bioscaffold for use in 3-D MTS and found advantages such as extensive porosity, homogeneous pore size, and amide linkages during cross-linking, in addition to cost-affordability. 20 …”

Section: Introductionmentioning

confidence: 99%

Characterization of Hen’s Egg White To Use It as a Novel Platform To Culture Three-Dimensional Multicellular Tumor Spheroids

et al. 2020

Self Cite

View full text Add to dashboard Cite

We are standardizing protocols to develop egg white (EW) as a cost-effective platform for culture of three-dimensional (3-D) multicellular tumor spheroids for application in understanding tumor microenvironments and drug screening. In this article, we describe several physical and physiological characteristics of EW to use it as 3-D cell culture platform. Field emission scanning electron microscopy revealed the presence of different microstructures. Hydrodynamic size distribution data indicated nano- and micron-sized particles. Rheological measurements revealed the viscosity and viscoelastic behavior appropriate for maintaining cell viability and supporting 3-D cell growth under high-sheer conditions. It was found that thereis no autofluorescence, a requirement for imparting transparency and for microscopic observations of the spheroids. The EW facilitated the development of 3-D tumor spheroids, with an emphasis of difference in cell proliferation and intercellular cytoskeletal organization between two-dimensional and 3-D spheroid cultures. Put together, EW proves to be a cost-affordable and simple platform for 3-D culture of tumor spheroids.

show abstract

Fabrication and characterization of egg white cryogel scaffold for three-dimensional (3D) cell culture

Cited by 11 publications

References 16 publications

Egg White Cryogel for Removal of Methylene Blue from Aqueous Solution

Egg White Cryogel for Removal of Methylene Blue from Aqueous Solution

An insight on egg white: From most common functional food to biomaterial application

Characterization of Hen’s Egg White To Use It as a Novel Platform To Culture Three-Dimensional Multicellular Tumor Spheroids

Contact Info

Product

Resources

About