Fabrication and characterization of DTBP-crosslinked chitosan scaffolds for skin tissue engineering

Adekogbe, Iyabo; Ghanem, Amyl

doi:10.1016/j.biomaterials.2005.05.043

Cited by 195 publications

(127 citation statements)

References 31 publications

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“…2), by their sealed surface or sheet-like structure. However, the maximum tensile strength values of the scaffolds made from the collagen/chitosan (7:3) were in the range of ∼8 to ∼10 MPa which is within the range of the recommended values 13 . Therefore, these scaffolds should have a strength adequate to resist rupture during implantation and maintain integrity during in vivo/in vitro cell growth.…”

Section: Mechanical Properties and Morphologysupporting

confidence: 70%

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Faikrua¹,

Jeenapongsa²,

Sila-asna³

et al. 2009

ScienceAsia

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ABSTRACT:The aim of this study was to determine the properties of β-glycerol phosphate (GP)/collagen/chitosan blended films for the potential application to skin tissue engineering. Various ratios of collagen to chitosan (8:2 and 7:3) and amounts of GP (0.5, 1, and 1.5% w/w) of total polymers were blended in solution form and cast into films. According to SEM images, the casted films showed a non-porous surface. For the mechanical properties, the prepared scaffolds exhibited the maximum elongation ranging from 15-23%, which is lower than those found by other researchers. However, the maximum tensile strength values of the scaffolds made from the collagen/chitosan (ratios 7:3) crosslinked with 0.5 or 1% w/w GP were in the range of 8-10 MPa which achieve the recommended values for application in skin tissue engineering. The scaffolds showed ability to retain their structure after immersion in phosphate buffer saline solution (pH 7.4) for 1 h, and their volume increased about 20%. After incubation in collagenase solution (200 U of collagenase/5 g of collagen) at 37°C, the scaffolds were degraded within 24 to 26 days which coincides very well with the healing time of acute wounds (about 25 days). FT-IR studies revealed the possibility of an interaction of GP with collagen/chitosan via ionic interaction that enhances the strength and stability of the prepared scaffold. The results from an in vitro culture study showed that the keratinocyte HaCaT culture could adhere well and grow on the selected scaffold with a typical morphology at 98.1 ± 1.8% of the control (cells growth on tissue culture plate) after cultivation for 5 days. The results suggest the potential of the GP/collagen/chitosan blended films for use as skin scaffolds.

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Section: Mechanical Properties and Morphologysupporting

confidence: 70%

“…Several methods have been tried to optimize its biodegradation rate and the mechanical properties. These include blending of collagen with other natural polymers [10][11][12] and/or introducing other chemical agents to the collagen [13][14][15][16][17] . Glutaraldehyde is used as the common crosslinking agent.…”

Section: Introductionmentioning

confidence: 99%

Untitled

Faikrua¹,

Jeenapongsa²,

Sila-asna³

et al. 2009

ScienceAsia

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“…Its maximum tensile strength (8.5 ± 0.1 MPa) lies within the range of 8-10 MPa previously recommended 18,24 , although the elongation tolerated (2.4 ± 0.6%) was lower than reported previously 25,26 . Additionally, the scaffolds were easy to handle and resisted rupture during implantation.…”

Section: Discussionmentioning

confidence: 83%

Efficacy evaluation of the fibroblast-seeded collagen/chitosan scaffold on application in skin tissue engineering

Intaraprasit¹,

Faikrua²,

Sittichokechaiwut³

et al. 2012

ScienceAsia

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There remains a need for dressings which aid the healing of chronically refractory wounds. To this end, we formulated and tested a collagen/chitosan scaffold supporting basic fibroblast growth factor (bFGF) producing cells. Collagen was blended with chitosan derived from crab shell (in ratio 7:3 of 3% weight of total polymer), crosslinked with glutaraldehyde, and then cast into a film. Tensile strength and elongation at break values of the scaffold was 8.5 ± 0.1 MPa and 2.4 ± 0.6%, respectively. The swelling degree was 77 ± 4%, and the weight remaining after collagenase degradation for 1 month was 30.5 ± 6.3%. Our scaffolds showed bio-compatibility because they encouraged attachment and proliferation of spindle-shaped human dermal fibroblasts (HDFs) which secreted surface-bound bFGF. Next, the fibroblast-seeded scaffold was prepared by seeding HDFs on the collagen/chitosan scaffold (5 × 10 5 cells/cm 2 ) and further cultured for 7 days.These fibroblast-seeded scaffolds were used to dress full depth wounds in domestic pigs. The wounds with fibroblastseeded scaffolds showed reduced swelling at day 7 and at all time points (7, 14, and 21 days), and re-epithelialization was faster than controls. Thus at day 21 of treatment, wounds treated with the fibroblast-seeded scaffold provided the highest percent re-epithelialization (69.7 ± 9.6%), which was higher than untreated (49.9 ± 6.3%) and cell-free scaffold treatment (54.3 ± 6.9%). The wound tissue of the fibroblast-seeded scaffold treated group showed regular arrangement of host collagens and the rete ridge-like structure at the epidermal layer was also found at day 21. The results indicate the potential of the fibroblast-seeded scaffold for application in skin tissue engineering.

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“…The samples were then removed at 5 minutes time interval and the superficial water was gently blotted on a filter paper and weighed. The amount of water uptaken by the lyophilised gel was calculated by measuring the reduced level of water (ml) in the measuring cylinder 25 .…”

Section: Seperation Of Aloe Vera Gelmentioning

confidence: 99%

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2018

IJPSR

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Photochemoprotection has nowadays become inevitable to combat aging. Exposure to Ultraviolet radiations accelerates skin aging which eventually culminates in wrinkles, laxity, dyspigmentation, roughness and dryness. Though many active synthetic topicals have been used since years, the present era of treating an aged skin has been diverted towards natural biomaterials as these synthetic topicals pose health and safety risk on human health. In an effort to produce a promising natural anti-aging agent in contemporary cosmetics, this study has been aimed to formulate an anti-aging gel by blending three biopolymers namely Collagen (COL) 3%w/v, Chitosan (CS) 1.5% w/v and Aloe vera (AV) gel 0.21% w/v. The AV blended COL-CS gel was characterized by spreadability and moisture uptake test, which indicated good spreadability and increased hydrophyllicity of the gel. Cell culture studies on NIH3T3 mouse fibroblasts cells were carried out using senescenceassociated-β-gal [SA-β-gal] as a biomarker. The fibroblasts cells on incubation with AV blended COL-CS gel showed increased proliferation rate and the absence of blue stain indicated that the process of senesce has been reversed. In conclusion, the prepared AV blended COL-CS gel helps in regeneration and rejuvenation of the skin and therefore can be used as a promising anti-aging gel.

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Fabrication and characterization of DTBP-crosslinked chitosan scaffolds for skin tissue engineering

Cited by 195 publications

References 31 publications

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Efficacy evaluation of the fibroblast-seeded collagen/chitosan scaffold on application in skin tissue engineering

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