Fabrication and characterization of disposable type lactate oxidase sensors for dairy products and clinical analysis

Patel, N. G.; Erlenkötter, Ansgar; Cammann, Karl; Chemnitius, Gabriele-Christine

doi:10.1016/s0925-4005(00)00410-x

Cited by 82 publications

(52 citation statements)

References 26 publications

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“…These techniques, although highly sensitive and accurate, may require laborious sample treatments and expensive instrumentation. For the detection of l-lactate, four enzymes are commonly used: Lactate dehydrogenase [7 -9], Lactate oxidase (LOx) [10], Cytochrome b2 [11,12] and Lactate monooxidase [13]. For the first three biocatalysts, pyruvate is the enzymatic product, whereas for the last enzyme, acetate is obtained.…”

Section: Introductionmentioning

confidence: 99%

An L‐Lactate Amperometric Enzyme Electrode Based on L‐Lactate Oxidase Immobilized in a Laponite Gel on a Glassy Carbon Electrode. Application to Dairy Products and Red Wine

et al. 2010

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Section: Introductionmentioning

confidence: 99%

An L‐Lactate Amperometric Enzyme Electrode Based on L‐Lactate Oxidase Immobilized in a Laponite Gel on a Glassy Carbon Electrode. Application to Dairy Products and Red Wine

et al. 2010

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“…[20][21][22] For immunosensors based on electrochemical methods, it is also possible to miniaturize the electrode for field applications. 19 Wide varieties of electrodes such as the carbon paste electrode (CPE), 17,18,25,26 glassy carbon electrode, 20 platinum electrode 27 and gold electrode, 28 have been used for electrochemical immunosensors. Recently, several immunosensor devices have been developed on screen-printed electrodes (SPEs).…”

Section: Introductionmentioning

confidence: 99%

Amperometric Immunosensor for the Detection of Vibrio cholerae O1 Using Disposable Screen-printed Electrodes

et al. 2006

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A disposable amperometric immunosensor was studied for the rapid detection of Vibrio cholerae (V. cholerae), the causative agent of cholera, employing an indirect sandwich enzyme linked immunosorbent assay (ELISA) principle. Screen-printed electrodes (SPEs) were fabricated (by using commercial and homemade carbon inks), electrochemically characterized and the assay conditions were optimized for capturing antibodies and antigen. Whole cell lysate (WCL) of V. cholerae was used to raise antibodies in rabbits and mice. The antibodies raised against WCL of V. cholerae were found to be specific, and no cross reactivity was observed with other enteric bacteria. 1-Naphthyl phosphate was used as a substrate with the amperometric detection of its enzymatic hydrolysis product 1-naphthol at a potential of +400 mV vs. Ag/AgCl reference electrode. A comparison between the amperometric detection technique and the standard ELISA was made in terms of the total assay time, the amount of biological materials used and the sensitivity of detection. The minimum detection limit of the amperometric immunosensor for V. cholerae was found to be 10 5 cells/ml in 55 min, while ELISA detected 10 6 cells/ml in 4 h.

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“…2 μL of the stock solution was dropped on the Pt-Nafion WE surface. The sensor was kept at 4 • C, in humid atmosphere, overnight [21].…”

Section: Electrochemical Measurementsmentioning

confidence: 99%

A Flexible Front-End for Wearable Electrochemical Sensing

Hanitra

Lobello

Stradolini

et al. 2018

2018 IEEE International Symposium on Medical Measurements and Applications (MeMeA)

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Abstract-This work presents the design and the realization of a flexible front-end circuitry for electrochemical sensing with wearable devices. The hardware combines readout circuitry for amperometric and Open Circuit Potential (OCP) measurements. The sensing platforms are dedicated to lactate and lithium detection in sweat, hence allowing the monitoring of athletes under physical effort. The wearability of the system is ensured by the flexibility of the electronic substrate, its small dimensions that fit an armband case, and the wireless transmission through a Bluetooth Low Energy (BLE) module. The power consumption of the system has been evaluated to be 200 mW, with 3.6 V on board power supply.

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Fabrication and characterization of disposable type lactate oxidase sensors for dairy products and clinical analysis

Cited by 82 publications

References 26 publications

An L‐Lactate Amperometric Enzyme Electrode Based on L‐Lactate Oxidase Immobilized in a Laponite Gel on a Glassy Carbon Electrode. Application to Dairy Products and Red Wine

An L‐Lactate Amperometric Enzyme Electrode Based on L‐Lactate Oxidase Immobilized in a Laponite Gel on a Glassy Carbon Electrode. Application to Dairy Products and Red Wine

Amperometric Immunosensor for the Detection of Vibrio cholerae O1 Using Disposable Screen-printed Electrodes

A Flexible Front-End for Wearable Electrochemical Sensing

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