Fabrication and characterization of curcumin-loaded silk fibroin/P(LLA-CL) nanofibrous scaffold

Lian, Yuan; Zhan, Jie; Zhang, Kuihua; Mo, Xiumei

doi:10.1007/s11706-014-0270-8

Cited by 42 publications

(25 citation statements)

References 23 publications

(25 reference statements)

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“…[15][16][17][18] Numerous studies have proposed electrospun meshes loaded with curcumin, given the potential for health care applications. A wide range of polymers has been used, including polycaprolactone (PCL), [19][20][21] polylactic acid (PLA), 22 polylactic-co-glycolic acid, 23 polyvinylpyrrolidone (PVP), 24 silk, 25 and cellulose acetate. 26 Because of the hydrophobic nature of curcumin, the use of hydrophobic polymers such as degradable polyesters is particularly attractive to achieve a slow and prolonged release.…”

Section: Introductionmentioning

confidence: 99%

“…Relatively high amounts of curcumin have been incorporated in electrospun materials, as most of the studies carried out propose proportions of curcumin 1% of the polymer weight. [19][20][21][22]25,28 These materials often lead to concentrations near or above the apoptosis threshold of 10 μM. Although several authors claim benefits from such materials to stimulate repair, it is not clear why these amounts have been selected while data from studies carried out with free curcumin suggest that lower concentrations might be more adequate for wound healing applications.…”

Section: Introductionmentioning

confidence: 99%

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Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications

Mouthuy¹,

Škoc²,

Gašparović³

et al. 2017

IJN

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Electrospun filaments represent a new generation of medical textiles with promising applications in soft tissue repair. A potential strategy to improve their design is to combine them with bioactive molecules. Curcumin, a natural compound found in turmeric, is particularly attractive for its antioxidant, anti-inflammatory, and antimicrobial properties. However, investigating the range of relevant doses of curcumin in materials designed for tissue regeneration has remained limited. In this paper, a wide range of curcumin concentrations was explored and the potential of the resulting materials for soft tissue repair applications was assessed. Polydioxanone (PDO) filaments were prepared with various amounts of curcumin: 0%, 0.001%, 0.01%, 0.1%, 1%, and 10% (weight to weight ratio). The results from the present study showed that, at low doses (≤0.1%), the addition of curcumin has no influence on the spinning process or on the physicochemical properties of the filaments, whereas higher doses lead to smaller fiber diameters and improved mechanical properties. Moreover, filaments with 0.001% and 0.01% curcumin stimulate the metabolic activity and proliferation of normal human dermal fibroblasts (NHDFs) compared with the no-filament control. However, this stimulation is not significant when compared to the control filaments (0%). Highly dosed filaments induce either the inhibition of proliferation (with 1%) or cell apoptosis (with 10%) as a result of the concentrations of curcumin found in the medium (9 and 32 μM, respectively), which are near or above the known toxicity threshold of curcumin (~10 μM). Moreover, filaments with 10% curcumin increase the catalase activity and glutathione content in NHDFs, indicating an increased production of reactive oxygen species resulting from the large concentration of curcumin. Overall, this study suggested that PDO electrospun filaments loaded with low amounts of curcumin are more promising compared with higher concentrations for stimulating tissue repair. This study also highlighted the need to explore lower concentrations when using polymers as PDO, such as those with polycaprolactone and other degradable polyesters.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications

Mouthuy¹,

Škoc²,

Gašparović³

et al. 2017

IJN

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“…Our results revealed that remarkably high encapsulation (>97 %) and loading (10% -80%) efficiency of CUR can be achieved in the CMSPs fabrication process, while typical loading efficiency for hydrophobic compound loading in protein nanoparticles is around 5% [47][48][49][50][51] .This phenomenon could owe to the strong hydrophobic interaction between hydrophobic CUR and the water-insoluble silk-II structure formed during the salting-out process. Our results have shown significant improvement in loading efficiency (up to 80%) compared with 6% found for CUR-loading into SF/poly(L-lactic acid-co-e-caprolactone) (P(LLA-CL)) nanofibrous scaffolds 52 , 12% for the silk/CUR nanoparticles fabricated in supercritical CO2 53 , 15% for the SFPs reported by Li et al 54 , 30% for the silk/CUR nanoparticles produced by desolvation method 55 , as well as 10% loading efficiency for the paclitaxel loaded SFPs 56 . The lower loading efficiency in SFPs via desolvation method was due to the fact that CUR tend to dissolve in organic solvent instead of adsorb on SF nanoparticles SFPs.…”

Section: Discussionmentioning

confidence: 73%

Magnetic-Silk Core–Shell Nanoparticles as Potential Carriers for Targeted Delivery of Curcumin into Human Breast Cancer Cells

Song

Muthana

Mukherjee

et al. 2017

ACS Biomater. Sci. Eng.

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Curcumin is a promising anti-cancer drug but its applications in cancer therapy are limited due to its poor solubility, short half-life and low bioavailability. In this paper, we present a curcumin loaded magnetic silk fibroin core-shell nanoparticle system for sustained release of curcumin into breast cancer cells. Curcumin loaded magnetic silk fibroin core-shell nanoparticles were fabricated by a simple salting-out method using sodium phosphate with magnetic nanoparticles. The size, zeta potential, encapsulation/ loading efficiency and curcumin release rate were controlled and optimised by regulating silk fibroin concentration, pH value of the phosphate solution and curcumin usage. Curcumin loaded magnetic silk fibroin core-shell nanoparticles showed enhanced cytotoxicity and higher cellular uptake in the human Caucasian breast adenocarcinoma cell line (MDA-MB-231cells) evidenced by MTT and cellular uptake assays. In addition, silk fibroin nanoparticles and magnetic silk fibroin nanoparticles without curcumin loaded were used as controls. The particles prepared using sodium phosphate showed significantly smaller diameter (90-350 nm) compared with those prepared using potassium phosphate, which possess a diameter range of 500-1200 nm. These smaller particles are superior for biomedical applications since such size range is particularly desired for cell internalization. In addition, the magnetic cores inside the particles provide possibility of using an external magnet for cancer targeting.

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“…Afterwards, the curcumin would be sustained released along the degradation of microspheres. Therefore, there may be two main mechanisms that regulate the release behavior of curucmin, diffusion and carrier's degradation [38,54]. Comparing among different microsphere formulations, the release rate of curcumin was not significantly different.…”

Section: Time (H)mentioning

confidence: 98%

Development of Thai Silk Fibroin/Hyaluronic Acid Microspheres and the Application on Controlled Release of Curcumin

Sungkhaphan¹,

Ratanavaraporn²,

Damrongsukkul³

2017

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Abstract. The aim of this study was to develop microspheres from Thai silk fibroin (SF) blended with hyaluronic acid (HA) for controlled release application. HA was introduced to blend with SF to enhance properties of the microspheres. The SF-based microspheres could be prepared by water in oil emulsion and glutaraldehyde crosslinking technique. The obtained microspheres contained 71-91% SF and 9-29% HA. The microspheres having higher SF content showed more stability and slower degradation rate. Then, to prove the potential of SFHA microspheres as carriers for drug delivery, curcumin was absorbed in the microspheres. All microspheres released curcumin in a controllable manner, possibly due to the hydrophobic interaction between curcumin and crystalline domain of SF. Furthermore, it was shown that HA was self-released from the microspheres. The GAcrosslinked SFHA microspheres that simultaneously and controlled released curcumin and HA could be further developed and applied as injectable drug delivery system for treatment of diseases e.g. arthritis.

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Fabrication and characterization of curcumin-loaded silk fibroin/P(LLA-CL) nanofibrous scaffold

Cited by 42 publications

References 23 publications

Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications

Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications

Magnetic-Silk Core–Shell Nanoparticles as Potential Carriers for Targeted Delivery of Curcumin into Human Breast Cancer Cells

Development of Thai Silk Fibroin/Hyaluronic Acid Microspheres and the Application on Controlled Release of Curcumin

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