This paper presents a development in producing low residual stress PECVD SiNX layers at high deposition rates and their biomedical. The key factor in the novel process is the employment ofup to 600W high powers in high frequency (13.56 MJHz). In conjunction with the adjustment of the reactant gases composition, the residual stress can be tuned to 4MPa and high deposition rate up to 320nm/min can be achieved. Moreover, by using this optimized process, an 11ium thick low stress SiNX layer was produced, which will be used to fabricate large area windowsfor cell culture. Finally, a cell culture test by cultivating mouse stem cells onto porous membrane by the low stress PECVD SiNX layers also indicated that these layers are biocompatible and are suitable for biomedical applications.