Fabrication and Characterization of a Reversed-Phase/Strong Cation Exchange Stationary Phase Gradient

Cain, Caitlin N.; Weatherbee, Shelby L.; Forzano, Anna V.; Rutan, Sarah C.; Collinson, Maryanne M.

doi:10.1016/j.chroma.2020.461177

Cited by 7 publications

(2 citation statements)

References 46 publications

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“…However, the inorganic salt used in SCX is not compatible with the later ionization source (typically an electrospray ionization (ESI) source), which limits its wide application. ,− As one type of hydrophobic interaction chromatography (HIC) method, the separation mechanism of HILIC is orthogonal to that of RP chromatography. However, HILIC requires peptides to be soluble in high level organic solvents, which is not applicable for many peptides. ,− Since high pH RP chromatography does not require an additional desalting step before LC-MS analysis, it becomes a popular method used as the first dimension separation. − Nevertheless, high-pH RPLC and low-pH RPLC are not perfectly orthogonal to each other, since both methods separate peptides based on peptide hydrophobicity. − …”

Section: Introductionmentioning

confidence: 99%

Deep Bottom-up Proteomics Enabled by the Integration of Liquid-Phase Ion Trap

Hong

Zhai

et al. 2023

Anal. Chem.

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In bottom-up proteomics, the complexity of the proteome requires advanced peptide separation and/or fractionation methods to acquire an in-depth understanding of protein profiles. Proposed earlier as a solution-phase ion manipulation device, liquid phase ion traps (LPITs) were used in front of mass spectrometers to accumulate target ions for improved detection sensitivity. In this work, an LPIT-reversed phase liquid chromatography-tandem mass spectrometry (LPIT-RPLC-MS/MS) platform was established for deep bottom-up proteomics. LPIT was used here as a robust and effective method for peptide fractionation, which also shows good reproducibility and sensitivity on both qualitative and quantitative levels. LPIT separates peptides based on their effective charges and hydrodynamic radii, which is orthogonal to that of RPLC. With excellent orthogonality, the integration of LPIT with RPLC-MS/MS could effectively increase the number of peptides and proteins being detected. When HeLa cells were analyzed, peptide and protein coverages were increased by ∼89.2% and 50.3%, respectively. With high efficiency and low cost, this LPIT-based peptide fraction method could potentially be used in routine deep bottom-up proteomics.

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Section: Introductionmentioning

confidence: 99%

Deep Bottom-up Proteomics Enabled by the Integration of Liquid-Phase Ion Trap

Hong

Zhai

et al. 2023

Anal. Chem.

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“…Compared with single-mode stationary phases, the mixed-mode stationary phases that possess multiple chromatographic ligands have attracted substantial attention owing to their superior selectivity and separation efficiency for complex mixtures of analytes [6][7][8]. Great efforts have been made in designing and fabricating advanced mixed-mode stationary phases [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Preparation of spherical silica hydroxyl‐functionalized covalent organic polymer composites for mixed‐mode liquid chromatography

Chen

Wang

Fan

et al. 2022

J of Separation Science

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Covalent organic polymers are an emerging class of amorphous microporous materials that have raised increasing concerns in analytical chemistry due to their unique structural and surface chemical properties. However, the application of covalent organic polymers as mixed-mode stationary phases in chromatographic separations has rarely been reported. Herein, novel spherical silica hydroxyl-functionalized covalent organic polymer composites were successfully prepared via a layer-by-layer approach. The structure and morphology of the materials were carefully characterized by elemental analysis, Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, X-ray diffraction, thermogravimetric analysis, Brunauer-Emmett-Teller, and contact angle measurements. Baseline separations of various alkylbenzenes, polycyclic aromatic hydrocarbons, and nucleosides and bases were achieved on the prepared stationary phase under reversedphase/hydrophilic interaction mode. The column efficiencies of 23 853 and 36 580 plates/m were obtained for butylbenzene and uracil, respectively, and the relative standard deviation of the retention time for continuous injections was less than 1.38% (n = 10), suggesting satisfactory column efficiency and repeatability. Additionally, this novel stationary phase realized the complete separation of the endocrine-disrupting chemicals in river water. This work affords a new route for synthesizing covalent organic polymers-based mixed-mode stationary phase and further reveals their great potential in chromatographic separation.

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Dendritic organic molecular gel coating with molecular shape selectivity and its application in selective separation by liquid chromatography

et al. 2022

Anal Bioanal Chem

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Fabrication and Characterization of a Reversed-Phase/Strong Cation Exchange Stationary Phase Gradient

Cited by 7 publications

References 46 publications

Deep Bottom-up Proteomics Enabled by the Integration of Liquid-Phase Ion Trap

Deep Bottom-up Proteomics Enabled by the Integration of Liquid-Phase Ion Trap

Preparation of spherical silica hydroxyl‐functionalized covalent organic polymer composites for mixed‐mode liquid chromatography

Dendritic organic molecular gel coating with molecular shape selectivity and its application in selective separation by liquid chromatography

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