Fabrication and characterization of a Layered Double Hydroxide based catalase biosensor and a catalytic sensor for hydrogen peroxide determination

Tomassetti, M.; Pezzilli, Riccardo; Prestopino, Giuseppe; Natale, Corrado Di; Medaglia, P. G.

doi:10.1016/j.microc.2021.106700

Cited by 11 publications

(18 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a subsequent work, in order to overcome some practical issues in the measurement of real samples containing H 2 O 2 , a second type of enzymatic biosensor was also developed [ 35 ], again based on LDH and catalase, but using a Clark-type electrode rather than a GC electrochemical transducer. Despite the good results already obtained with the previously reported [ 34 ] enzymatic (GC-Ag paste )-LDH-catalase biosensor (linearity range 0.2–160 mmol L −1 ), the present work conversely deals with the possibility of significantly improving the performance of the more-simple (GC-Ag paste )-LDH non-enzymatic sensor. In fact, recent reports on non-enzymatic electrocatalytic sensors, usually based on nanocomposites, report outstanding performance in detecting hydrogen peroxide [ 36 , 37 , 38 , 39 , 40 ].…”

Section: Introductionmentioning

confidence: 71%

“…Among them, the so-called layered double hydroxides (LDHs), a class of inorganic materials with layered structure, have proven to be very useful and suitable for the development of electrocatalytic sensors [ 30 , 31 , 32 , 33 ]. In this regard, we recently published [ 34 ] the development of a glassy carbon (GC-Ag paste )-LDH-catalase enzyme biosensor and a non-enzymatic amperometric glassy carbon sensor of the same type, for the determination of hydrogen peroxide, both based on layered double hydroxide of the [Zn II Al III (OH) 2 ] + NO 3 − · nH 2 O type. The former, being a biosensor, naturally resulted in a greater sensitivity than the latter, in fact limits of detection (LOD) of 0.2 mmol L −1 and of 1.0 mmol L −1 were found, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…Among these, the ones that most impressed us are those sensors that used LDH [ 32 , 36 , 37 , 40 ] or make use of L-proline [ 38 , 39 , 41 ]. We therefore tested whether the response of our previous catalytic non-enzymatic sensor, not yet studied in depth [ 34 ], a glassy carbon-modified by LDH, the latter glued on the GC by means of silver paste, could be significantly improved by the presence of L-proline in solution. Indeed, the preliminary results have been very positive; in the present paper we studied this amperometric sensor thoroughly, also applying it to the determination of hydrogen peroxide in real matrices (human urines).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Novel Electrochemical Sensors Based on L-Proline Assisted LDH for H2O2 Determination in Healthy and Diabetic Urine

Tomassetti

Pezzilli

Prestopino

et al. 2022

Sensors

Self Cite

View full text Add to dashboard Cite

In this paper, a novel non-enzymatic modified glassy carbon (GC) sensor, of the (GC-Agpaste)-catalytic proline-assisted LDH type, for H2O2 determination was fabricated, studied, characterized and employed to determine the hydrogen peroxide content in healthy and diabetic human urine. LDH (whose composition can be schematized as [ZnIIAlIII (OH)2]+ NO3−·nH2O) is glued to glassy carbon by means of silver paste, while proline, which increases the catalytic properties of LDH, is used free in solution in the phosphate buffer. A voltametric survey was first conducted to ascertain the positive effect induced by the presence of proline, i.e., the increase of sensor sensitivity. Then a deep study of the new three-electrode amperometric proline-assisted LDH sensor, whose working electrode was of the same type as the one used to perform the cyclic voltammetry, was carried out, working at first in static air, then in a nitrogen atmosphere. Possible interferences from various substances, both oxidants and antioxidants, were also investigated. Lastly, the new amperometric sensor was successfully used to determine the H2O2 level in human urine from both healthy and diabetic subjects. The effect of proline in enhancing the properties of the sensor system was also investigated. The limit of detection (LOD) of the new catalytic sensor was of the order of 0.15 mmol L−1, working in air, and of 0.05 µmol L−1, working in nitrogen atmosphere.

show abstract

Section: Introductionmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Novel Electrochemical Sensors Based on L-Proline Assisted LDH for H2O2 Determination in Healthy and Diabetic Urine

Tomassetti

Pezzilli

Prestopino

et al. 2022

Sensors

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition, their active centers are often hidden inside a structure, where direct electron transfer between an enzyme and an electrode can often be difficult, and the environment on the electrode surface can also lead to reduced enzyme activity [ 127 , 128 ]. Currently, the molecular structures of enzymes can be modified by techniques such as chemical modification, recombinant enzyme molecules, and targeted mutagenesis [ 129 , 130 , 131 ], or by adjusting the modification process of enzyme molecules on the electrode surface to maintain the enzyme activity and expose the active center, thus improving the efficiency of the direct electron transfer of enzyme molecules [ 132 ].…”

Section: Commonly Used Signal Amplification Strategies For Electroche...mentioning

confidence: 99%

Electrochemical Signal Amplification Strategies and Their Use in Olfactory and Taste Evaluation

Wang

Liu

et al. 2022

Biosensors

View full text Add to dashboard Cite

Biosensors are powerful analytical tools used to identify and detect target molecules. Electrochemical biosensors, which combine biosensing with electrochemical analysis techniques, are efficient analytical instruments that translate concentration signals into electrical signals, enabling the quantitative and qualitative analysis of target molecules. Electrochemical biosensors have been widely used in various fields of detection and analysis due to their high sensitivity, superior selectivity, quick reaction time, and inexpensive cost. However, the signal changes caused by interactions between a biological probe and a target molecule are very weak and difficult to capture directly by using detection instruments. Therefore, various signal amplification strategies have been proposed and developed to increase the accuracy and sensitivity of detection systems. This review serves as a reference for biosensor and detector research, as it introduces the research progress of electrochemical signal amplification strategies in olfactory and taste evaluation. It also discusses the latest signal amplification strategies currently being employed in electrochemical biosensors for nanomaterial development, enzyme labeling, and nucleic acid amplification techniques, and highlights the most recent work in using cell tissues as biosensitive elements.

show abstract

“…Moreover, there is currently an increasing interest in LDHs due to their properties as heterogeneous catalysts and supports for molecular catalysts [29][30][31] or photocatalysis [32], for fabrication of superhydrophobic surfaces [33,34], water treatment and remediation [35][36][37] and ion-exchanger membranes [38,39]. Interestingly, anion conductivity in LDHs can be modulated by incorporating different anions in the interlayer space, allowing for their use as sensors [40][41][42][43][44] or as fillers in different electrochemical applications, such as fuel cells, supercapacitors and water splitting [45][46][47]. Different types of LDH compounds and LDH-based nanocomposites and hybrids have been made available by several synthesis methods, including exfoliation or topdown methods, as well as several chemical or mechanical methods (see, for instance, [48,49]).…”

Section: Introductionmentioning

confidence: 99%

Layered Double Hydroxides as a Drug Delivery Vehicle for S-Allyl-Mercapto-Cysteine (SAMC)

et al. 2021

Self Cite

View full text Add to dashboard Cite

The intercalations of anionic molecules and drugs in layered double hydroxides (LDHs) have been intensively investigated in recent years. Due to their properties, such as versatility in chemical composition, good biocompatibility, high density and protection of loaded drugs, LDHs seem very promising nanosized systems for drug delivery. In this work, we report the intercalation of S-allyl-mercapto-cysteine (SAMC), which is a component of garlic that is well-known for its anti-tumor properties, inside ZnAl-LDH (hereafter LDH) nanostructured crystals. In order to investigate the efficacy of the intercalation and drug delivery of SAMC, the intercalated compounds were characterized using X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). The increase in the interlayer distance of LDH from 8.9 Å, typical of the nitrate phase, to 13.9 Å indicated the intercalation of SAMC, which was also confirmed using FT-IR spectra. Indeed, compared to that of the pristine LDH precursor, the spectrum of LDH-SAMC was richly structured in the fingerprint region below 1300 cm−1, whose peaks corresponded to those of the functional groups in the SAMC molecular anion. The LDH-SAMC empirical formula, obtained from UV-Vis spectrophotometry and thermogravimetric analysis, was [Zn0.67Al0.33(OH)2]SAMC0.15(NO3)0.18·0.6H2O. The morphology of the sample was investigated using SEM: LDH-SAMC exhibited a more irregular size and shape of the flake-like crystals in comparison with the pristine LDH, with a reduction in the average crystallite size from 3 µm to about 2 µm. In vitro drug release studies were performed in a phosphate buffer solution at pH 7.2 and 37 °C and were analyzed using UV-Vis spectrophotometry. The SAMC release from LDH-SAMC was initially characterized by a burst effect in the first four hours, during which, 32% of the SAMC is released. Subsequently, the release percentage increased at a slower rate until 42% after 48 h; then it stabilized at 43% and remained constant for the remaining period of the investigation. The LDH-SAMC complex that was developed in this study showed the improved efficacy of the action of SAMC in reducing the invasive capacity of a human hepatoma cell line.

show abstract

Fabrication and characterization of a Layered Double Hydroxide based catalase biosensor and a catalytic sensor for hydrogen peroxide determination

Cited by 11 publications

References 48 publications

Novel Electrochemical Sensors Based on L-Proline Assisted LDH for H2O2 Determination in Healthy and Diabetic Urine

Novel Electrochemical Sensors Based on L-Proline Assisted LDH for H2O2 Determination in Healthy and Diabetic Urine

Electrochemical Signal Amplification Strategies and Their Use in Olfactory and Taste Evaluation

Layered Double Hydroxides as a Drug Delivery Vehicle for S-Allyl-Mercapto-Cysteine (SAMC)

Contact Info

Product

Resources

About