Fabrication and biological properties of calcium phosphate/chitosan composite coating on titanium in modified SBF

Kim, Seok-Jae; Jeong, Young-Hwa; Moon, Hyun-Joo; SongHoJun,; Park, Kyung Hee

doi:10.1016/j.msec.2018.04.060

Cited by 25 publications

(18 citation statements)

References 20 publications

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“…Additionally, chitosan nanocomposites have been developed as prominent materials used for the loading and controlled release of growth factors over time, but few studies have been conducted on mHA/CS (Qasim et al, 2017a;Park et al, 2018). The mHA/CS could be an appropriate carrier for rhAm, which has been proved in our study.…”

Section: Discussionmentioning

confidence: 60%

Mesoporous Hydroxyapatite/Chitosan Loaded With Recombinant-Human Amelogenin Could Enhance Antibacterial Effect and Promote Periodontal Regeneration

Liao

Shu

et al. 2020

Front. Cell. Infect. Microbiol.

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The recovery of impaired periodontium is still a challenge to the treatment of periodontitis. This study was the first to apply the mesoporous hydroxyapatites/chitosan (mHA/CS) composite scaffold to periodontal regeneration. The aim of our study is to evaluate the biological effects of mesoporous hydroxyapatite/chitosan (mHA/CS) loaded with recombinant human amelogenin (rhAm) on periodontal regeneration. The physicochemical properties of mHA/CS scaffolds were examined by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET) analysis. Then, the biological effects of the mHA/CS loaded with rhAm were evaluated, including antibacterial effect, controlled-release capacity, osteogenic and cementogenic effects in vitro and in vivo. The antibacterial effect was tested on 1.5 mg/mL CS; 3 mg/mL mHA; 2.25 mg/mL mHA/CS; 4.5 mg/mL mHA/CS and 20 µg/mL rhAm. Tryptic Soy Broth culture medium was used as a baseline control. Osteogenic effect of rhAm (20 µg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 µg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media. The hPDLCs treated either with osteogenic media or Dulbecco's modified Eagle's medium (DMEM) alone were used as the baseline control. In the animal model, 4week-old nude mice (BALB/c) (n = 6) implanted with root slices subcutaneously were used to observe the cementogenic effect in vivo. The root slices were treated with rhAm (20 µg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 µg/mL rhAm). The root slices treated with osteogenic medium alone were used as the baseline control. The analyses showed that the mHA/CS particles were 2 µm in diameter and had a uniform pore size. Liao et al. A Research on Periodontal Regeneration The mesoporous structure was 7 nm in diameter and its surface area was 33.95 m 2 /g. The scaffold exhibited antibacterial effects against Fusobacterium nucleatum and Porphyromonas gingivalis. The mHA/CS scaffold sustainably released rhAm. The mHA/CS loaded with 20 µg/mL rhAm upregulated ALP activity, the expression levels of osteogenesis-related genes and proteins in vitro. Additionally, it promoted the formation of cementum-like tissue in vivo. Our findings suggest that mHA/CS loaded with 20 µg/mL rhAm could inhibit the growth of periodontal pathogens and promote the formation of bone and cementum-like tissue.

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Section: Discussionmentioning

confidence: 60%

Mesoporous Hydroxyapatite/Chitosan Loaded With Recombinant-Human Amelogenin Could Enhance Antibacterial Effect and Promote Periodontal Regeneration

Liao

Shu

et al. 2020

Front. Cell. Infect. Microbiol.

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“…When the PEG/CS mass ratio was 1/0.1, combined with the influence of surface material wettability and surface point position, the nano PEG/CS coating exhibited the best antibacterial performance and showed significant long-term colony inhibition efficiency (93.3%, 7 days) ( Figure 4 ) [ 69 ]. The CaP/CS coating was prepared by incubating the spin-coated CS layer on the titanium substrate in modified simulated body fluid after 1M NaOH post-treatment, and the hydrophilicity of the sample was significantly increased and the apatite morphology was also transferred onto ellipsoidal clusters [ 70 ]. Compared to the HA/CS hybrid coating, the CaP/CS hybrid coating demonstrated better cell viability, adhesion, and differentiation of MG-63 cells [ 70 ].…”

Section: Application Of Nano-cs In Bone Tissue Engineeringmentioning

confidence: 99%

The Application of Chitosan Nanostructures in Stomatology

2021

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Chitosan (CS) is a natural polymer with a positive charge, a deacetylated derivative of chitin. Chitosan nanostructures (nano-CS) have received increasing interest due to their potential applications and remarkable properties. They offer advantages in stomatology due to their excellent biocompatibility, their antibacterial properties, and their biodegradability. Nano-CSs can be applied as drug carriers for soft tissue diseases, bone tissue engineering and dental hard tissue remineralization; furthermore, they have been used in endodontics due to their antibacterial properties; and, finally, nano-CS can improve the adhesion and mechanical properties of dental-restorative materials due to their physical blend and chemical combinations. In this review, recent developments in the application of nano-CS for stomatology are summarized, with an emphasis on nano-CS’s performance characteristics in different application fields. Moreover, the challenges posed by and the future trends in its application are assessed.

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“…Nitrite accumulation in an aqueous environment and at optimum pH and temperature conditions can lead to producing more toxic compounds that have higher toxicity than nitrate. On the other hand, these two compounds and also by converting to ammonia and nitrate can cause eutrophication phenomena in aqueous environments [3]. Water used in aquaculture includes organic and mineral materials, soluble minerals, dissolved gases, suspended materials, dissolved organic compounds, and microorganisms that affect the quality of aquaculture water.…”

mentioning

confidence: 99%

“…Removal of phosphate and ammonia is one of the important aspects of wastewater treatments. Phosphorus has been successfully removed from the effluent by various treatments, such as chitosan and various nanocomposites [3,7]. It has been known for many decades that the presence of phosphate and ammonia compounds in water can be completely injurious to the environment.…”

mentioning

confidence: 99%

“…The results showed that this nanocomposite had high efficiency in nitrate and phosphate removal. In a study on the adsorption in order to remove phosphate using chitosan with titanium dioxide from an aqueous solution, the results showed that this composite was a useful adsorbent for phosphate removal from aqueous solution [3]. Thus, in this study, chitosan and activated carbon nanocomposite adsorbent were prepared to remove organic pollutants from the fish farm effluents.…”

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confidence: 99%

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Isothermal and Kinetic Evaluation of Adsorption Fish Farm Effluents by Nanocomposites (Chitosan and Activated Carbon)

Rezaei

Rastegar

Hedayati

et al. 2021

J Adv Environ Health Res

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Background: In wastewater treatment, removal of phosphate and ammonia is of great importance. Chitosan is a copolymer, which can be applied in low-cost adsorption. Thus, in this study, chitosan and activated carbon nanocomposite adsorbents were prepared to remove organic pollutants from the fish farm effluents. Methods: This study was performed at different physicochemical conditions of pH (5-8), effluent dose (25-100 mg/L), and contact time (15-90 min) minutes. Adsorption isotherm studies were analyzed using Freundlich, Langmuir models, and adsorption kinetics studies. Results: The Langmuir and Freundlich isotherms for nitrite (R2=0.9076, R2=0.5911), phosphate (R2=0.9307, R2=0.5755), and ammonia (R2=0.7288 and R2=0.7549) were respectively obtained. According to the results, the data of nitrite and phosphate pollutants were more consistent with the Langmuir model, but the data of ammonia pollutants were more consistent with the Freundlich. The best optimal adsorption occurred at a pH=7. Elevation of the initial concentration of the pollutant led to the depletion of the removal functions. With increasing the contact time, adsorption efficiency increased. Conclusion: Finally, with respect to the obtained elimination percentage (R=99.98%), chitosan and activated carbon nanocomposites have a high ability to remove organic pollutants.

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Fabrication and biological properties of calcium phosphate/chitosan composite coating on titanium in modified SBF

Cited by 25 publications

References 20 publications

Mesoporous Hydroxyapatite/Chitosan Loaded With Recombinant-Human Amelogenin Could Enhance Antibacterial Effect and Promote Periodontal Regeneration

Mesoporous Hydroxyapatite/Chitosan Loaded With Recombinant-Human Amelogenin Could Enhance Antibacterial Effect and Promote Periodontal Regeneration

The Application of Chitosan Nanostructures in Stomatology

Isothermal and Kinetic Evaluation of Adsorption Fish Farm Effluents by Nanocomposites (Chitosan and Activated Carbon)

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