Fabrication and Biocompatibility of Rutin-containing PHBV Nanofibrous Scaffolds

Abstract

초록: 루틴은 항발암, 소염제, 항바이러스성 기능을 갖는 물질이다. 미생물이 만들어낸 폴리에스테르인 PHBV와 루틴 을 전기방사하여 나노섬유 부직포를 얻었다. 나노섬유 부직포의 항균성은 황색포도상구균(Staphylococcus aureus), 폐 렴간균(Klebsiella pneumoniae)을 사용하여 평가하였고, KB 셀을 이용하여 세포독성을 평가하였다. 그 결과 루틴을 3 wt% 함유할 때 지지체는 우수한 항균성을 보였으며, KB 셀을 이용한 실험결과로부터 루틴을 함유하는 PHBV 지지체 는 세포독성을 나타내지 않음을 알 수 있었다.Abstract: Rutin(R) exhibits a wide range of biological activities including anticarcinogenic, antiinflammatory and antiviral actions. The purpose of this study is to investigate the effect of rutin concentrations (1 and 3 wt%) on the antibacterial activity of poly(3-hydroxybutylate-co-hydroxyvalerate)(PHBV) scaffolds. Antibacterial activity was evaluated by using Staphylococcus aureus and Klebsiella pneumoniae. Furthermore, the qualitative ongrowth of human KB endothelial cells was done to study in vitro cytotoxicity of the scaffolds. As the results, PHBV scaffolds containing 3 wt% rutin completely inhibited the proliferation of Staphylococcus aureus and Klebsiella pneumoniae. In addition, the PHBV/R scaffolds used in this study did not show any cytotoxicity when evaluated them with KB endothelial cells.

Recent Development of Polyhydroxyalkanoates (PHA)-Based Materials for Antibacterial Applications: A Review

“…Regarding incorporating Ag NPs into submicron polymer fibers, only a few studies have been reported, such as poly­( l -lactide), ethylene vinyl alcohol, poly­(ε-caprolactone), polyacrylonitrile, or polypropylene . Furthermore, few studies about the preparation of Ag NPs and polyhydroxy­butyrate electrospun fibers. , For example, Castro-Mayorga et al used the electrospinning coating technique to develop active PHA–Ag NPs with bactericidal efficiency against Salmonella enterica . Similarly, PHB–Ag NPs composite was synthesized by Jayakumar et al The nanocomposite was stable, polydisperse, and exhibited high antimicrobial resistance against E. coli and Pseudomonas sp.…”

Rheological Measurements of Physical Gelation in a Crystallized Poly(3-hydroxybutyrate)/Poly(p-vinylphenol) Blends

“…As a matter of fact, the blend of PHB with PVPh is miscible 25,[27][28][29][30][31][32][33] and the miscibility, crystallization and morphology of this system have been widely affected by intermolecular hydrogen bonding interaction. The presence of the hydrogen bonding between PHB and PVPh may confine the movement between the chain segments, reduce interchains convolution and block the entanglement between chains of the blends.…”

“…When they are mixed together, it has been evidenced that the PHB/PVPh blends displayed a single-albeit broad-glass transition temperature (T g ), which indicates the blends are miscible at amorphous region. As a classic blend sample, it has been reported in many studies, 25,27,28 regarding to its miscibility and crystallization. It indicated that the addition of PVPh prevented crystallization of PHB in the blends, of course, it meant that PVPh decreased the crystalline melting temperature and degree of crystallinity of PHB in the blends.…”

“…[17][18][19][20][21][22][23][24] Poly(3-hydroxybutyrate) (PHB) is a crystalline polymer and poly(p-vinylphenol) (PVPh) is wholly amorphous. [25][26][27] In our previous works, 28 it showed that the PHB/PVPh blends displayed a single-albeit broad-glass transition temperature (T g ), which indicates the miscibility of the blends at amorphous region. The powder WAXD experiments evidenced that, when PHB content is more than 60%, the PHB can crystallize in the blends and the crystallinity of PHB increases with the addition of PHB contents.…”

Fabrication and Characterization of Collagen-Immobilized Porous PHBV/HA Nanocomposite Scaffolds for Bone Tissue Engineering

“…The morphology of the composite scaffolds was examined using a field emission scanning electron microscope (FE-SEM S4300, Hitachi, Tokyo, Japan). [25].…”