Fabrication and application of zwitterionic phosphorylcholine functionalized monoliths with different hydrophilic crosslinkers in hydrophilic interaction chromatography

ZIC-cHILIC Functionalized Magnetic Nanoparticle for Rapid and Sensitive Glycopeptide Enrichment from <1 µL Serum

“…Phosphocholine is a relatively new zwitterion used in the HILIC approach (ZIC-cHILIC), and has the opposite charge arrangement compared to the commercially available ZIC-HILIC (sulfobetaine). Because the positive charge is located on the outermost part of the particle, ZIC-cHILIC has more accessible positive charge to enhance the electrostatic attraction of negatively charged glycopeptides, especially towards negatively charged sialylated glycopeptide [ 33 , 34 ]. Wang et al studied the physicochemical and chromatographic properties of phosphocholine-functionalized monolith columns; a total of 24 glycopeptides, including 19 fucosylated and 2 sialyl-glycopeptide, were enriched from human IgG, suggesting a promising material for N-glycopeptide enrichment [ 33 ].…”

“…Because the positive charge is located on the outermost part of the particle, ZIC-cHILIC has more accessible positive charge to enhance the electrostatic attraction of negatively charged glycopeptides, especially towards negatively charged sialylated glycopeptide [ 33 , 34 ]. Wang et al studied the physicochemical and chromatographic properties of phosphocholine-functionalized monolith columns; a total of 24 glycopeptides, including 19 fucosylated and 2 sialyl-glycopeptide, were enriched from human IgG, suggesting a promising material for N-glycopeptide enrichment [ 33 ]. Although a vast number of glycoproteomic workflows have been developed, many suffer from the time-consuming and complicated enrichment process.…”

Supported Metal Nanoparticles in Metal‐Organic Monoliths for Assembly of a Catalytic Microfluidic Reactor

“…The macro‐ and mesopores are present in porous monoliths and are beneficial for rapid mass transfer, high flow rates and low column back pressure [21] . In recent years, organic and inorganic monoliths have been overgrew their use in flow reactors [19,22–24] and chromatography separation [25,26] . Usually composite monoliths of porous materials are in situ prepared by mixing with organic polymers or inorganic silica inside the column [24,25] …”

“…[21] In recent years, organic and inorganic monoliths have been overgrew their use in flow reactors [19,[22][23][24] and chromatography separation. [25,26] Usually composite monoliths of porous materials are in situ prepared by mixing with organic polymers or inorganic silica inside the column. [24,25] Metal-organic gels (MOGs) are an attractive catalogue of porous materials which are self-assembled from metal ions and organic ligands through metal-organic coordination, and have found applications in catalysis.…”

“…[25,26] Usually composite monoliths of porous materials are in situ prepared by mixing with organic polymers or inorganic silica inside the column. [24,25] Metal-organic gels (MOGs) are an attractive catalogue of porous materials which are self-assembled from metal ions and organic ligands through metal-organic coordination, and have found applications in catalysis. [27][28][29][30][31] As a catalogue of hierarchically porous materials, MOGs provide both meso-/macropores for mass transfer channels and micropores for the immobilization of catalytically active centers.…”