Fabricating polystyrene fiber-dehydrogenase assemble as a functional biocatalyst

An, Hongjie; Jin, Bo; Dai, Sheng

doi:10.1016/j.enzmictec.2014.09.010

Cited by 18 publications

(14 citation statements)

References 28 publications

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“…Desirable PSNFs can be obtained using 20% (w/v) PS regardless of the applied voltage or distance. These findings were in agreement with the data reported by An et al (2015). At the lowest polymer concentration (10%, w/v), liquid droplets were produced instead of fibers, probably due to a high surface tension which is associated with a low viscosity of the liquid (Deitzel et al, 2001).…”

Section: Fabrication and Functionalization Of Polystyrene Nanofiberssupporting

confidence: 92%

“…Oxidation using HNO 3 has shown a favourable surface modification for protein adsorption (Kaur and Suri, 2007). In a previous study, different ratios of HNO 3 /H 2 SO 4 for modifying polystyrene nanofiber surface were reported (An et al, 2015) and an acid mixing ratio of 4:1 (HNO 3 /H 2 SO 4 ) was found to significantly improve the enzyme loading. However, the acidic solution containing sulphuric acid resulted in fiber aggregation.…”

Section: Fabrication and Functionalization Of Polystyrene Nanofibersmentioning

confidence: 97%

“…The surface of polystyrene nanofibers (PS) was modified through surface oxidation to introduce oxygen-containing reactive groups of carboxyl (COOH) and hydroxyl (OH) for enzyme binding (Ros et al, 2002). An approximately 1 cm × 2.5 cm piece of nanofibers was immersed in HNO 3 (69%) for 2 h at room temperature (An et al, 2015). The acid-modified support was rinsed with water and PBS (pH 7.2) three times to remove excess acid.…”

Section: Enzyme Immobilization On Nanofibersmentioning

confidence: 99%

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Manipulation of nanofiber-based β-galactosidase nanoenvironment for enhancement of galacto-oligosaccharide production

Misson

Dai

Jin

et al. 2016

Journal of Biotechnology

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The nanoenvironment of nanobiocatalysts, such as local hydrophobicity, pH and charge density, plays a significant role in optimizing the enzymatic selectivity and specificity. In this study, Kluyveromyces lactis β-galactosidase (Gal) was assembled onto polystyrene nanofibers (PSNFs) to form PSNF-Gal nanobiocatalysts. We proposed that local hydrophobicity on the nanofiber surface could expel water molecules so that the transgalactosylation would be preferable over hydrolysis during the bioconversion of lactose, thus improve the galacto-oligosaccharides (GOS) yield. PSNFs were fabricated by electro-spinning and the operational parameters were optimized to obtain the nanofibers with uniform size and ordered alignment. The resulting nanofibers were functionalized for enzyme immobilization through a chemical oxidation method. The functionalized PSNF improved the enzyme adsorption capacity up to 3100 mg/g nanofiber as well as enhanced the enzyme stability with 80% of its original activity. Importantly, the functionalized PSNF-Gal significantly improved the GOS yield and the production rate was up to 110 g/l/h in comparison with 37 g/l/h by free β-galactosidase. Our research findings demonstrate that the localized nanoenvironment of the PSNF-Gal nanobiocatalysts favour transgalactosylation over hydrolysis in lactose bioconversion.

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Section: Fabrication and Functionalization Of Polystyrene Nanofiberssupporting

confidence: 92%

Section: Fabrication and Functionalization Of Polystyrene Nanofibersmentioning

confidence: 97%

Section: Enzyme Immobilization On Nanofibersmentioning

confidence: 99%

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Manipulation of nanofiber-based β-galactosidase nanoenvironment for enhancement of galacto-oligosaccharide production

Misson

Dai

Jin

et al. 2016

Journal of Biotechnology

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“…For our nanofiber/β-galactosidase hybrids, although minor cracks are noticed on 2 h treated nanofibers, all enzyme-loading nanofibers have a nearly identical thickness, which means that a thin layer of enzyme coating uniformly covers the PSNF surface ( Figure 1e,f at 20 µm and 50 µm magnification, respectively). This thin layer is formed by the enzyme molecule interaction with functional groups distributed on the nanofiber surface that is characterized by our previous reports [7,26]. A similar layer of homogenous antibody coating was recently reported on electrospun polyethersulfone nanofibrous membrane due to hydrophobic interactions [28].…”

Section: Physical Characterization Of Nanofibers/β-galactosidase Assesupporting

confidence: 59%

“…The chemical characteristics of the nanofibers play an important role in β-galactosidase binding and enzyme-catalyzed reactions. To introduce functional groups for enzyme immobilisation and tune the nanoenvironment for enzyme reaction, the PSNF surface was treated by concentrated HNO 3 (63%) for 2, 10 and 24 h to generate oxygen-containing functional groups such as carboxyl (COOH) and hydroxyl (OH) to facilitate β-galactosidase binding [26]. The effect of surface modifications on mechanical stability and wettability of the nanofibers was first examined.…”

Section: Textural Properties Of Polymer Nanofibersmentioning

confidence: 99%

Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides

et al. 2020

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Molecular distribution, structural conformation and catalytic activity at the interface between enzyme and its immobilising support are vital in the enzymatic reactions for producing bioproducts. In this study, a nanobiocatalyst assembly, β-galactosidase immobilized on chemically modified electrospun polystyrene nanofibers (PSNF), was synthesized for converting lactose into galacto-oligosaccharides (GOS). Characterization results using scanning electron microscopy (SEM) and fluorescence analysis of fluorescein isothiocyanat (FITC) labelled β-galactosidase revealed homogenous enzyme immobilization, thin layer structural conformation and biochemical functionalities of the nanobiocatalyst assembly. The β-galactosidase/PSNF assembly displayed enhanced enzyme catalytic performance at a residence time of around 1 min in a disc-stacked column reactor. A GOS yield of 41% and a lactose conversion of 88% was achieved at the initial lactose concentration of 300 g/L at this residence time. This system provided a controllable contact time of products and substrates on the nanofiber surface and could be used for products which are sensitive to the duration of nanobiocatalysis.

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Electrospinning and Photocrosslinking of Highly Modified Fungal Chitosan

Christ

Menzel

2022

Macro Materials & Eng

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The main focus of this study is to elucidate and optimize the electrospinning process for highly modified fungal chitosan. An efficient one‐step process for functionalization of chitosan with arylazide and other desired functional groups via amidation is used for synthesis. Critical electrospinning process parameters, namely, molecular weight, concentration, and ratio of chitosan and additive poly(ethylene oxide) as well as degree of substitution of chitosan are identified by systematic parameter variation following design‐of‐experiment guidelines. Their influence on the viscoelastic properties of spinning solutions is studied and attributed to changes in chain entanglements. These changes result in drastic shifts in the electrohydrodynamic jet behavior and the resulting fiber morphologies. When the viscosity is increased above a critical limit, complete cancellation of whipping instabilities is observed, resulting in a stable linear jet and highly aligned but partly coalescing microfibers. It is shown how this process conditions can be avoided and how the production of uniform and defect‐free nanofibers from highly functional chitosan can be carried out. In addition, a new photocrosslinking method for generation of water and acid stable chitosan nanofiber meshes is established.

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Fabricating polystyrene fiber-dehydrogenase assemble as a functional biocatalyst

Cited by 18 publications

References 28 publications

Manipulation of nanofiber-based β-galactosidase nanoenvironment for enhancement of galacto-oligosaccharide production

Manipulation of nanofiber-based β-galactosidase nanoenvironment for enhancement of galacto-oligosaccharide production

Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides

Electrospinning and Photocrosslinking of Highly Modified Fungal Chitosan

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