Fabp4 contributes toward regulating inflammatory gene expression and oxidative stress in Ctenopharyngodon idella

Lei, Chunyan; Xie, Yujing; Li, Shengjie; Jiang, Peng; Du, Jianguo; Tian, Jingjing

doi:10.1016/j.cbpb.2022.110715

Cited by 5 publications

(4 citation statements)

References 40 publications

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“…Therefore, High-ω3 might have repressed specific components of Atlantic salmon’s innate immune system [e.g., cxcl8 at 24 h post-poly(I:C) injection] and promoted others (e.g., lyz2 at 24 h post-Asal injection). FABP4 and LXRA are involved in the lipid metabolism-inflammation crosstalk in mammalian and fish leukocytes 21 , 22 , 79 , 80 . FABP4 promotes intracellular cholesterol accumulation and pro-inflammatory signaling 79 , 80 , whereas LXRA promotes cholesterol efflux and inhibits inflammation 21 , 22 .…”

Section: Discussionmentioning

confidence: 99%

“…FABP4 and LXRA are involved in the lipid metabolism-inflammation crosstalk in mammalian and fish leukocytes 21 , 22 , 79 , 80 . FABP4 promotes intracellular cholesterol accumulation and pro-inflammatory signaling 79 , 80 , whereas LXRA promotes cholesterol efflux and inhibits inflammation 21 , 22 . However, fabp4 and lxra showed inconsistent responses to Asal or poly(I:C) –only salmon fed the High-ω6 showed down-regulated lxra transcript levels 24 h post-poly(I:C) injection.…”

Section: Discussionmentioning

confidence: 99%

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Vegetable omega-3 and omega-6 fatty acids differentially modulate the antiviral and antibacterial immune responses of Atlantic salmon

Caballero-Solares,

Eslamloo,

Hall

et al. 2024

Sci Rep

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The immunomodulatory effects of omega-3 and omega-6 fatty acids are a crucial subject of investigation for sustainable fish aquaculture, as fish oil is increasingly replaced by terrestrial vegetable oils in aquafeeds. Unlike previous research focusing on fish oil replacement with vegetable alternatives, our study explored how the omega-6 to omega-3 polyunsaturated fatty acid (PUFA) ratio in low-fish oil aquafeeds influences Atlantic salmon's antiviral and antibacterial immune responses. Atlantic salmon were fed aquafeeds rich in soy oil (high in omega-6) or linseed oil (high in omega-3) for 12 weeks and then challenged with bacterial (formalin-killed Aeromonas salmonicida) or viral-like (polyriboinosinic polyribocytidylic acid) antigens. The head kidneys of salmon fed high dietary omega-3 levels exhibited a more anti-inflammatory fatty acid profile and a restrained induction of pro-inflammatory and neutrophil-related genes during the immune challenges. The high-omega-3 diet also promoted a higher expression of genes associated with the interferon-mediated signaling pathway, potentially enhancing antiviral immunity. This research highlights the capacity of vegetable oils with different omega-6 to omega-3 PUFA ratios to modulate specific components of fish immune responses, offering insights for future research on the intricate lipid nutrition-immunity interplay and the development of novel sustainable low-fish oil clinical aquaculture feeds.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Vegetable omega-3 and omega-6 fatty acids differentially modulate the antiviral and antibacterial immune responses of Atlantic salmon

Caballero-Solares,

Eslamloo,

Hall

et al. 2024

Sci Rep

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“…The specific operation steps were recommended by the reagent manufacturer. The gene expression was amplified in triplicate using the Step-One-plus real-time PCR system (Applied Biosystems, Foster City, CA, United States) according to our previous study ( Lei et al, 2022 ). The amplification mixture was comprised of 1 μl (25 ng) cDNA template, 0.8 μl each of forward and upstream primers, 10 μl 2 × Power SYBR™ Green PCR Master Mix (Thermo Fisher Scientific Co., Ltd., Shanghai, China), and 7.4 μl sterilized ultrapure water.…”

Section: Methodsmentioning

confidence: 99%

Different responses to glucose overload between two strains of largemouth bass (Micropterus salmoides)

et al. 2022

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In order to improve the glucose utilization capacity of largemouth bass (Micropterus salmoides), responses to glucose overload between two strains (Y: breeding strain; W: wild strain) were compared at 0, 6, 12, and 24 h after glucose injection (1.67 g/kg). The data revealed that plasma glucose in the Y strain (<12 h) recovered faster than in the W strain (12 h), with the Y strain secreted more insulin within 6 h post-injection. Triglyceride (TG) and low-density lipoprotein-cholesterol (VLDL-CH) content in the Y strain increased, peaking at 12 h, then decreased, whereas the W strain’s TG content was not affected and VLDL-CH content decreased. The hepatic and muscular fatty acid synthetase, liver x receptor-1, and sterol regulatory element-binding protein expressions were consistent with the TG content change. Both strains’ liver and muscle glycogen contents exhibited similar trends to that of the glycogen synthase gene—increasing, then declining, and peaking at 6 and 12 h. The expression levels of hepatic and muscular phosphofructokinase and pyruvate kinase in the Y strain increased, peaking at 12 h. In the W strain, they were suppressed and reached the minimum at 24 h. The mRNA levels of hepatic and muscular phosphoenolpyruvate carboxykinase and glucose-6-phosphatase were enhanced and peaked at 24 h in both strains, hepatic isocitrate dehydrogenase-1, and α-ketoglutarate dehydrogenase complex expression increased after declining, peaking at 12 and 24 h. Two genes in the W strain’s muscles showed a similar trend. Both strains’ transcriptome results identified seven common functional genes for resistance to hyperglycemia that were involved in the circadian rhythm pathway, which is a suggested key pathway for coping with hyperglycemia. Furthermore, 48 differential genes were identified between the two strains, and these genes were enriched in the TGF-beta and cell cycle signaling pathways, indicating that these pathways may be key factors affecting the differential responses to glucose overload. We conducted a comprehensive comparison of glucose overload molecular responses between two strains of M. salmoides, and the results can provide a promising strategy to improve the glucose utilization capacity of M. salmoides based on advantageous pre-existing traits.

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“…It is worth noting that the gene fabp4 expression (fatty acid-binding protein-4) was induced in the gills of greater amberjack when exposed to 10 or 40 ppt saline. In fish, Fabp4 can bind to the marine environmental toxins perfluoroalkyl acids (PFAAs), trigger the undesirable uptake and accumulation (Lei et al, 2022), which has been demonstrated to suppress lysozyme activity in marine organisms, then interrupt their resistance to bacterial infection (Betts, 2007). Furthermore, PFAA can easily enter human bodies via the food chain and disturb human hormonal signaling, which poses potential risks to food safety and public health (Betts, 2007;McComb et al, 2020), which highlights the importance of a well-controlled rearing condition during the aquaculture to ensure the food safety of greater amberjack.…”

Section: Immune Responsementioning

confidence: 99%

Divergent molecular responses of greater amberjack (Seriola dumerili) to acute salinity stress revealed by comparative transcriptome analysis

et al. 2023

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Greater amberjack (Seriola dumerili) is an important commercial fish for its high growth rate and excellent flesh quality. However, its sensitivity to variations of water salinity poses challenges to the cage culture. In this study, the greater amberjack were reared in the optimum salinity (30 ppt, CK) and undesired regimes (10 and 40 ppt) for 72 hours. The molecular adaptive mechanisms to salinity stress were revealed by the comparative transcriptome analysis for the gills and kidneys. In gills, a total of 445 and 423 differentially expressed genes (DEGs) were identified in 10 and 40 ppt salinity stress groups, respectively. Those DEGs were involved in cartilage and skeletal development, ions transport, and immune response. The major ion secretion and osmoregulation transport proteins gene slc12a2/nkcc1 and cftr expression levels were significantly down-regulated at 10 ppt, but slightly activated at 40 ppt, compared with the control group. The expression changes in response to the Na+, K+ movement, and Cl- ion secretion reduced under the hypo-osmotic exposure and ion excretion boost upon hyper-salinity stress. Meanwhile, the cartilage and skeletal development were enhanced in the gills by hypo- or hyper-salinity stimuli, which is critical for maintaining gill structures and improving respiration and osmoregulation under salinity stress. In kidneys, 600 and 539 DEGs were identified in 10 and 40 ppt groups, respectively. Those DEGs were enriched in oxygen transport, pronephros development, regulation of growth, blood coagulation, ion transmembrane transport, and immune response. While the known renal Na+/Cl– co-transporter gene slc12a3/ncc expression level was significantly down-regulated at 10 ppt, the organic cation transporter 2 gene slc22a2, ammonium transmembrane transport gene rhd and rhag expression levels were overexpressed under the hyper-salinity condition at 40 ppt, contributing to the salts secretion and ammonium transport regulation, to combat the osmotic influx of salts following the drink of seawater and elevated ammonia production upon high salinity stress. These findings advance our knowledge of adaptative mechanisms to the salinity stress and provide theoretical guidance for the optimal breeding mode for the aquaculture of greater amberjack.

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Fabp4 contributes toward regulating inflammatory gene expression and oxidative stress in Ctenopharyngodon idella

Cited by 5 publications

References 40 publications

Vegetable omega-3 and omega-6 fatty acids differentially modulate the antiviral and antibacterial immune responses of Atlantic salmon

Vegetable omega-3 and omega-6 fatty acids differentially modulate the antiviral and antibacterial immune responses of Atlantic salmon

Different responses to glucose overload between two strains of largemouth bass (Micropterus salmoides)

Divergent molecular responses of greater amberjack (Seriola dumerili) to acute salinity stress revealed by comparative transcriptome analysis

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