Fabio Weintraub

Weintraub, Fabio

doi:10.11606/issn.2447-8997.teresa.2010.116818

Cited by 3 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A specific polyclonal antiserum to hCH was prepared by passing the neat anti-hGH RS 1-0 through an affinity column containing Sepharose 4B, to which hCS had been coupled by modification of the methods of Cuatrecasas et al (45), Porath et al (46) and Weintraub (47). The efficiency of the purification of the antiserum was established by monitoring the binding t o 12SI-hGH and "'I-hCS tracer obtained with increasing dilutions of the different antiserum fractions.…”

Section: Monospecific Antiserummentioning

confidence: 99%

Structure‐function studies on human growth hormone

Aubert

Bewley

Grumbach

et al. 1986

International Journal of Peptide and Protein Research

View full text Add to dashboard Cite

The relationship between the conformation of human pituitary growth hormone (hGH), biological activity, and ligand binding activity was studied by comparing conformational details previously published on in vivo and in vitro studies of identical samples of hGH and its known derivatives. In vivo assays included the rat tibia test for somatotropic activity and the pigeon crop‐sac assay for lactogenic hormone activity. Relative binding affinities were compared in radioimmuno‐assays using 125I‐hGH as tracer with 1) anti‐human chorionic somatomammotropin (hCS) serum (low discriminatory hybrid assay), 2) anti‐hGH sera (in conventional assays), 3) monospecific anti‐hGH serum (absence of cross‐reaction with hCS) and 4) human anti‐hGH sera obtained from GH‐deficient patients on replacement therapy. In addition, binding affinities were examined in two receptor‐binding assays, one specific for somatotropic activity (rabbit liver membranes, 125I‐hGH), and the other, for lactogenic hormones (rabbit mammary membranes, 125I‐oPRL). The conformational properties of native hGH and various chemically and enzymatically modified derivatives of the hormone were evaluated primarily from circular dichroism spectra, while conformational stabilities were estimated from the relative rates of tryptic digestion. Unfragmented, but chemically modified derivatives, exhibited good parallelism between retention or loss of native conformation and the in vivo potencies and in vitro binding affinities. None of the fragments of hGH showed activity in any of the radioreceptor assays or radioimmunoassays. Two derivatives of hGH, which contain gaps of 6 or 12 residues in the polypeptide backbone produced by partial enzymatic digestion, had full or increased in vivo potencies, full activities in the radioimmunoassays, and were the most active derivatives in both radio‐receptor assays. One of these, missing the hexapeptide corresponding to residues 135–140, was also found to retain nearly all the conformational properties of native hGH. These studies proved further evidence that 1) retention by modified forms of hGH of a high degree of in vivo biological potency or in vitro binding affinity is causally related to the retention of most of the conformation and conformational stability of the molecule, and 2) the biologically acitve, receptor‐binding and immunoreactive sites on the hGH molecule are 3‐dimensional in nature.

show abstract

Section: Monospecific Antiserummentioning

confidence: 99%

Structure‐function studies on human growth hormone

Aubert

Bewley

Grumbach

et al. 1986

International Journal of Peptide and Protein Research

View full text Add to dashboard Cite

show abstract

“…Despite stressing that it is important to maintain good working relationships, for instance, Fincher claims that he ‘protect[s] for a different thing than a producer protects for’ (Weintraub, 2010). Discussing negotiations during the development phase specifically, Fincher argues that while producers and studio executives reduce costs to provide the minimum resources needed by the director to complete the picture, he protects ‘the last 10 percent, the finesse’, that the extra budget, time and resources offer (Weintraub, 2010). Although Fincher seeks to depict himself as an artist unconcerned about the bottom-line, his comment is symptomatic of a profit-seeking agenda where greater production values and higher budgets provide scope for improving his fee.…”

Section: Fincher and Anonymous: Before House Of Cardsmentioning

confidence: 99%

PackagingHouse of CardsandThe Knick: How talent intermediaries manage the indie-auteur brand to sell premium television

Stubbs

2020

Critical Studies in Television

View full text Add to dashboard Cite

The article explores the migration of indie-auteurs to television during the 2010s. Using David Fincher and Steven Soderbergh’s work on House of Cards and The Knick as case studies, the article argues that talent intermediaries such as producers and talent managers have sought to mobilize the indie-auteur, a branded identity and authorial discourse, to package premium television programmes to exploit growing competition between channels and platforms. The article also explores repercussions of the indie-auteur’s insertion in television as a mark of distinction, arguing that it becomes increasingly important to be aware of the systems behind it.

show abstract

“…Em todo caso, trata-se de uma boca na qual se localiza um molar que lateja por sua polpa estragada, e que logo tem seu canal exposto por uma broca. Uma vez finalizada a intervenção, o molar estará morto, e na lápide se escreverá o nome do narrador: um profissional, a boca ou quem mais entrar na disputa pela narrativa (Weintraub, 2007).…”

unclassified

‘Bocas trabalhadoras’ e os reparos possíveis em tempos de pandemia

Couto

Botazzo

2022

Trab. educ. saúde

View full text Add to dashboard Cite

Resumo Neste artigo, o objetivo foi realizar uma análise crítica da relação entre saúde bucal e trabalho. A reflexão emergiu do aumento expressivo na busca por atendimentos de urgência odontológica em uma unidade básica de saúde durante a pandemia pelo coronavírus, em que os sujeitos apresentavam algo em comum: a demissão de seus empregos em fábricas de calçados. Com apoio na teoria social crítica, efetuou-se uma análise sobre como o trabalho, entendido como categoria fundante do ser social, tem o seu sentido esvaziado nas sociedades capitalistas, operando mais como um obstáculo à saúde bucal na dinâmica do trabalho assalariado e alienado. Isso porque pôde-se perceber que os sujeitos recém-desempregados acumulavam diversas necessidades em saúde bucal, como infecções e inflamações que causavam sofrimentos, até então internalizados e escondidos para que pudessem manter seus empregos em um contexto de desemprego iminente, possibilitando assim que pudessem seguir reproduzindo a sua vida material. Pensar a relação entre saúde bucal e trabalho nos aproxima da compreensão do processo saúde-doença como socialmente determinado, quando as relações de trabalho explorado se materializam nas ‘bocas trabalhadoras’. Desse modo, vislumbra-se a democratização do acesso à saúde bucal entendida como o direito a uma vida plena.

show abstract

Fabio Weintraub

Abstract: Com o você pensa a relação entre o tem po literá rio e o tem po histórico?

Cited by 3 publications

References 0 publications

Structure‐function studies on human growth hormone

Structure‐function studies on human growth hormone

PackagingHouse of CardsandThe Knick: How talent intermediaries manage the indie-auteur brand to sell premium television

‘Bocas trabalhadoras’ e os reparos possíveis em tempos de pandemia

Contact Info

Product

Resources

About