F-type Pyocins are Diverse Non-Contractile Phage Tail-Like Weapons for Killing<i>Pseudomonas aeruginosa</i>

Saha, Senjuti; Ojobor, C. D.; MacKinnon, Erik Michael; North, Olesia I.; Bondy‐Denomy, Joseph; Lam, Joseph S.; Ensminger, Alexander W.; Maxwell, Karen L.; Davidson, Alan R.

doi:10.1101/2021.02.16.431561

Cited by 7 publications

(6 citation statements)

References 45 publications

(77 reference statements)

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“…Colicin gene clusters are encoded on plasmids while pyocins are typically located on chromosomal DNA [ 26 , 29 ]. Tailocins rely on receptor-binding proteins (RBPs) located on tail fibres or spikes for initial and specific interaction with susceptible bacteria [ 30 , 31 ]. Phages kill bacteria through a lytic, replicative cycle, whereas PTLBs kill the target cell through membrane depolarisation in a single hit mechanism [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation, Purification, and Characterisation of a Phage Tail-Like Bacteriocin from the Insect Pathogenic Bacterium Brevibacillus laterosporus

et al. 2022

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The Gram-positive and spore-forming bacterium Brevibacillus laterosporus (Bl) belongs to the Brevibacillus brevis phylogenetic cluster. Isolates of the species have demonstrated pesticidal potency against a wide range of invertebrate pests and plant diseases. Two New Zealand isolates, Bl 1821L and Bl 1951, are under development as biopesticides for control of diamondback moth and other pests. However, due to the often-restricted growth of these endemic isolates, production can be an issue. Based on the previous work, it was hypothesised that the putative phages might be involved. During investigations of the cause of the disrupted growth, electron micrographs of crude lysate of Bl 1821L showed the presence of phages’ tail-like structures. A soft agar overlay method with PEG 8000 precipitation was used to differentiate between the antagonistic activity of the putative phage and phage tail-like structures (bacteriocins). Assay tests authenticated the absence of putative phage activity. Using the same method, broad-spectrum antibacterial activity of Bl 1821L lysate against several Gram-positive bacteria was found. SDS-PAGE of sucrose density gradient purified and 10 kD MWCO concentrated lysate showed a prominent protein band of ~48 kD, and transmission electron microscopy revealed the presence of polysheath-like structures. N-terminal sequencing of the ~48 kD protein mapped to a gene with weak predicted amino acid homology to a Bacillus PBSX phage-like element xkdK, the translated product of which shared >90% amino acid similarity to the phage tail-sheath protein of another Bl published genome, LMG15441. Bioinformatic analysis also identified an xkdK homolog in the Bl 1951 genome. However, genome comparison of the region around the xkdK gene between Bl 1821L and Bl 1951 found differences including two glycine rich protein encoding genes which contain imperfect repeats (1700 bp) in Bl 1951, while a putative phage region resides in the analogous Bl 1821L region. Although comparative analysis of the genomic organisation of Bl 1821L and Bl 1951 PBSX-like region with the defective phages PBSX, PBSZ, and PBP 180 of Bacillus subtilis isolates 168 and W23, and Bacillus phage PBP180 revealed low amino acids similarity, the genes encode similar functional proteins in similar arrangements, including phage tail-sheath (XkdK), tail (XkdO), holin (XhlB), and N-acetylmuramoyl-l-alanine (XlyA). AMPA analysis identified a bactericidal stretch of 13 amino acids in the ~48 kD sequenced protein of Bl 1821L. Antagonistic activity of the purified ~48 kD phage tail-like protein in the assays differed remarkably from the crude lysate by causing a decrease of 34.2% in the number of viable cells of Bl 1951, 18 h after treatment as compared to the control. Overall, the identified inducible phage tail-like particle is likely to have implications for the in vitro growth of the insect pathogenic isolate Bl 1821L.

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Section: Introductionmentioning

confidence: 99%

Isolation, Purification, and Characterisation of a Phage Tail-Like Bacteriocin from the Insect Pathogenic Bacterium Brevibacillus laterosporus

et al. 2022

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“…15B, and Supp. Table 3 )(63), indicating pyocin induction in this condition. This likely explains the presence of plaque-like clearings on the hard agar (e.g.…”

Section: Resultsmentioning

confidence: 87%

Interspecies surfactants serve as public goods enabling surface motility inPseudomonas aeruginosa

Warrell,

Zarrella,

Machalek

et al. 2024

Preprint

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In most natural environments, bacteria live in polymicrobial communities where secreted molecules from neighboring species alter bacterial behaviors including motility, but such interactions are understudied.Pseudomonas aeruginosais a motile opportunistic pathogen that exists in diverse multispecies environments such as the soil and is frequently found in human wound and respiratory tract co-infections with other bacteria includingStaphylococcus aureus. Here we show thatP. aeruginosacan co-opt secreted molecules from other species for flagellar-based emergent motility. We found that in the presence of exoproducts fromS. aureusand other bacteria,P. aeruginosaswitched from swarming to an alternative form of motility where it slid and spread on semi-solid surfaces, and it showed similar behavior on hard agar where it was otherwise unable to move. Surfactants from these species were required for theP. aeruginosaemergent motility, and it was also induced by the addition of numerous exogenous biological and synthetic surfactants. Mutant analysis indicated that this motility was similar to a previously described mucin-basedP. aeruginosamotility, ‘surfing’, albeit with divergent regulation. Thus, our study establishes a majorP. aeruginosasurfing-like emergent motility where secreted surfactants from the host as well as neighboring bacterial and interkingdom species serve as public goods, allowingP. aeruginosato access different environmental niches.

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“…Pyocins of the R/F type have a large molecular weight. Pyocins of the S-type have a lower molecular weight 20 .…”

Section: Types Of Pyocinsmentioning

confidence: 99%

Soluble Antimicrobial Peptide Pyocin of Pseudomonas aeruginosa and its Therapeutics: A Review Article

Sura Saleem Alberman,

EssamFadel Al-wan Al-Jumaili

2022

Indian Journal of Forensic Medicine & Toxicology

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Pyocinsis a bacteriocin produced by a group of Gram-ngative bacteria that belongs to Pseudomonasspecies. Pyocin is classified as a two distinct families of pyocins. (i) S-type pyocins (colicin-like bacteriocins), (ii) Tailocins (high-molecular weight bacteriocins that resemble phage tails).The structure of S-type pyocin is similar to that of colicin except that many S-type pyocins have three domains. Under normal conditions, the expression of prtN is repressed by PrtR. Upon exposure to stress conditions, such as DNA damage by chemicals or ultraviolet irradiation, an activated RecA triggers autoproteolytic cleavage of PrtR, which abrogates prtN repression and leads to pyocinproduction. The outer membrane receptors for three pyocins have been identified. These are FpvAI, FpvAII and FptA, all of which are involved in the uptake of iron-siderophore complexes. Before being translocated through the membrane and killing their target.This is becoming increasingly important as microbial imbalances in the natural gut flora have been suggested to play a role in a range of chronic diseases such as inflammatory bowel disease, diabetes, obesity and rheumatoid arthritis.Pyocin has now been shown to have antimicrobial activity againstbacteria in a biofilm is a limiting factor in the successful treatment of a range of chronic infections.

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F-type Pyocins are Diverse Non-Contractile Phage Tail-Like Weapons for KillingPseudomonas aeruginosa

Cited by 7 publications

References 45 publications

Isolation, Purification, and Characterisation of a Phage Tail-Like Bacteriocin from the Insect Pathogenic Bacterium Brevibacillus laterosporus

Isolation, Purification, and Characterisation of a Phage Tail-Like Bacteriocin from the Insect Pathogenic Bacterium Brevibacillus laterosporus

Interspecies surfactants serve as public goods enabling surface motility inPseudomonas aeruginosa

Soluble Antimicrobial Peptide Pyocin of Pseudomonas aeruginosa and its Therapeutics: A Review Article

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