F-prime transfer and multiplication of sexduced cells

Haan, P. G. de; Stouthamer, A. H.

doi:10.1017/s0016672300003402

Cited by 40 publications

(10 citation statements)

References 11 publications

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“…on August 9, 2020 by guest http://jb.asm.org/ Downloaded from WALKER AND PITTARD at 32 C. It can be seen that the curve shows no "plateau" region which would represent completion of transfer in the pairs formed in the first 5 min, as is found in classical entry curves using Hfr donors and allowing 5 min for pair formation (9). The low cell density in the dilution flask (about 105 per ml) precludes the possibility of pairs forming after the dilution step; thus, the continued increase of Ilv+ recombinants with time must reflect the ability of the F ilv merogenote to replicate in the zygote population without requiring integration (10). This is in contrast to the transfer of a chromosomal marker by an Hfr, where multiplication of recombinants cannot occur until after the donor marker has been integrated in the recipient chromosome.…”

Section: Vol 100 1969 321mentioning

confidence: 95%

Temperature-Sensitive Conjugation-Defective F Factor in Escherichia coli

Walker

Pittard

1969

J Bacteriol

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A mutant strain of Escherichia coli K-12 which is unable to transfer genetic material at 42 C has been isolated. Although transfer is inhibited at this temperature, the formation of specific pairs is unaffected. This strain and its derivatives also show an altered sensitivity to certain ribonucleic acid-containing male specific phages at 42 C. Both phenotypic changes are reversed when a derepressed fi+ R factor is present in the same cell and a revertant which has regained both activities has been obtained. The nature of the temperature-sensitive defect and its effect on the conjugation process are discussed.

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Section: Vol 100 1969 321mentioning

confidence: 95%

Temperature-Sensitive Conjugation-Defective F Factor in Escherichia coli

Walker

Pittard

1969

J Bacteriol

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“…Normally, two F' elements cannot coexist in the same cell (1)(2)(3), and similarly, replication of an F' element is inhibited in an Hfr strain (1,4,5). This property is called incompatibility and may stem from the same control mechanism that limits the number of copies of an F' element to 1-2 per chromosome (6,7).…”

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confidence: 99%

Genetic and Physicochemical Characterization of Escherichia coli Strains Carrying Fused F′ Elements Derived from KLF1 and F57

Willetts

Bastarrachea

1972

Proc. Natl. Acad. Sci. U.S.A.

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Although the two F' elements, F57 (carrying his+) and KLF1 (carrying leu+), cannot normally coexist in the same cell, crosses between RecA-strains of Escherichia coli carrying these two elements gave rise, at a low frequency, to progeny carrying both the his + and leu + markers in an extrachromosomal state. Genetic studies showed that the his+ and leu+ markers were now linked, and centrifugation studies of F' DNA isolated from these strains confirmed the presence of a new species of DNA molecule of altered size. It is proposed that the his+ and leu + genes in these strains are covalently linked on a single "fused" F' element.

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“…The plates were also supplemented with histidine and proline, two amino acids required by the recipient. Colonies that grew after incubation at 28 C were candidates for containing an episome produced by the fusion of FilO rill and Flsf2lac+ because growth on this medium required the argE+, met+, and pur+ genes from FilO rif as well as the lac+ gene from Fts62lac+, and also because only one F' factor can exist stably in a cell (9,33). Although deletion of some DNA seems to occur during episomal fusion, probably because of the incompatibility of two sets of F replication genes (37), the rifr gene should be on the fused episome because rif lies between argE+ and pur+, two genes required for growth on the selective plates.…”

Section: Resultsmentioning

confidence: 99%

Method for the isolation of Escherichia coli K-12 mutants deficient in essential genes

Armstrong¹,

Herman²

1976

J Bacteriol

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We developed a general procedure for the induction and identification of mutations in chromosomal essential genes that are located in a diploid region of Escherichia coli K-12. The partial diploidy is conferred by an episome that is temperature sensitive for replication so that a mutant strain will form microcolonies at 42 C on complete media if an essential chromosomal gene in the diploid region is defective. Mutations identified by this procedure can be classified into cistrons by a complementation method devised for the purpose. To verify that the procedure works in practice, we fused an episome covering the rif region with an Fjlac+ and used the resulting temperature-sensitive episome to identify chromosomal mutations in essential functions near rif. As expected, a certain proportion of the mutations were in the rif gene, an essential gene that codes for the ,3 subunit of ribonucleic acid polymerase.

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F-prime transfer and multiplication of sexduced cells

Cited by 40 publications

References 11 publications

Temperature-Sensitive Conjugation-Defective F Factor in Escherichia coli

Temperature-Sensitive Conjugation-Defective F Factor in Escherichia coli

Genetic and Physicochemical Characterization of Escherichia coli Strains Carrying Fused F′ Elements Derived from KLF1 and F57

Method for the isolation of Escherichia coli K-12 mutants deficient in essential genes

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