F‐695 emission from the purified photosystem II chlorophyll <i>a</i>‐protein complex

Satoh, Kimiyuki

doi:10.1016/0014-5793(80)80021-4

Cited by 42 publications

(8 citation statements)

References 20 publications

(1 reference statement)

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“…Increasing the protein:lipid ratio during reconstitution of PSII fragments into artificial membranes also increases the probability of696 nm emission. These findings suggest that, although 696 nm fluorescence may emanate from artificial centers which form upon freezing (21), emission at this wavelength can monitor the microenvironment near PSII. Clearly, the Chl banding profiles of Figure 5A suggest that the microenvironment of PSII is altered by iron deficiency.…”

Section: Resultsmentioning

confidence: 96%

Organization and Function of Chlorophyll in Membranes of Cyanobacteria during Iron Starvation

Guikema¹,

Sherman²

1983

Plant Physiol.

155

117

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Cells of Anacystis nidulans strain R2 and of Synechococcus cedrorum were grown in an iron-deficient medium. Iron starvation induced several pronounced effects without influencing the viability of these cells. The phycocyanin and chlorophyll contents of these cells were depressed, and the absorption maxima of membrane-bound chlorophyll was blue-shifted by 5 nanometers. Cells showed a dramatic increase in original and in maximal chlorophyll fluorescence when monitored at room temperature. Low temperature chlorophyll fluorescence revealed a loss in fluorescence at 696 and 716 nanometers; much of the remaining fluorescence emission was at 686 nanometers. These chanes sugest an alteration of membrane composition and structure. This was documented by an electrophoretic analysis of iron-deficient membranes.%The prominent findings were: (a) large chlorophyll-protein complexes were not observed in iron-deficient membranes, although the chlorophyll-binding proteins were present; (b) the staining of acrylamide gels with 3,3',5,5'-tetramethylbenzidine plus peroxide indicated that iron deficiency led to a decrease in the quantity of cytochromes. These results support a stral model of the relation between fluorescence and chlorophyll organization in Anacystis. In addition, they suggest a method for studying cytochrome and chlorophyll protein assembly in these membranes. thesis of nuclear and chloroplastic gene products, and the subsequent assembly of these products into functional membranes (1 1). MATERIALS AND METHODSCells of Synechococcus cedrorum UTEX 1191 were obtained from the Indiana University culture collection, whereasAnacystis nidulans R2 was kindly provided by G. A. van Arkel, University of Utrecht, The Netherlands. These strains differed notably in such characteristics as cold sensitivity and efficiency of genetic transformation (23). However, no major differences were observed in the photosynthetic parameters described here or in the cellular responses to iron deficiency. Cells were routinely monitored for bacterial contamination by microscopic observation and by plating on nutrient agar.Cells were grown in shaking culture (120 rpm) in a manner described previously (7). Illumination was provided by banks of cool-white fluorescent lights at an intensity of approximately 0.4 mw/cm2 (YSI Radiometer). Optimal growth was obtained using Allen's BG-l 1 growth medium (1). Iron-deficient media was prepared by replacing the ferric ammonium citrate stock solution with equal molar amounts of ammonium citrate. Glassware Cells in buffer A were incubated with 10 mM EDTA and lysozyme (10 mg/ml; Sigma) for 2 to 3 h at 37°C. A membrane fraction was obtained by centrifugation after the osmotic rupture of these spheroplasts (8). The Chl-protein organization and the heme-dependent peroxidase profile of membranes were determined using LiDS-PAGE in a manner previously described (8). Polyacrylamide gradient gels were cast and were loaded with samples (80 ,g protein/well) which had been solubilized for 10 min with 1% LiDS (...

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Section: Resultsmentioning

confidence: 96%

Organization and Function of Chlorophyll in Membranes of Cyanobacteria during Iron Starvation

Guikema¹,

Sherman²

1983

Plant Physiol.

155

117

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“…The emission maximum of chloroplast thylakoids isolated from chlorina-f2 is lower, at 727 nm (34,38) or 722 nm (8) and appears to be the result of the isolation procedure and length of storage of the thylakoids after isolation. Similar effects may be responsible for the changes in the relative fluorescence intensities of F-685 and F-693 compared with F-727 in isolated chlorinaf2 thylakoids, since the fluorescence emission spectrum of wild-type leaves is similar to that obtained from isolated thylakoids (20,34,35,38,43).…”

Section: Low Temperature Fluorescence Emission Spectramentioning

confidence: 83%

“…There is, however, a temperature sensitive pea mutant which loses photosystem II activity and the 695 nm fluorescence emission peak when grown at 30 ~ (44). This peak can be enhanced in isolated thylakoids or photosystem II preparations by 1,10-phenanthroline (35), which may be a useful method for detecting the presence of the reaction centre of photosystem II in mutant thylakoids.…”

Section: Low Temperature Fluorescence Emission Spectramentioning

confidence: 99%

Macromolecular physiology of plastids XIV.Viridis mutants in barley: Genetic, fluoroscopic and ultrastructural characterisation

Simpson

Wettstein

1980

Carlsberg Res. Commun.

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“…It is noteworthy that fraction 2 and 3 spectra have longer wavelength maxima than those given by Satoh [17] or Wessels [27], using different purification procedures. Wavelength X (nm) Figure 5.…”

Section: Preparation Of Protein Pigment Complexesmentioning

confidence: 91%

A single step separation of PS 1, PS 2 and chlorophyll-antenna particles from spinach chloroplasts

1982

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After solubilization of photosynthetic membranes by digitonin, three main protein pigment complexes were isolated by electrophoresis with deoxycholate as detergent.The band with the slowest mobility, fraction 1, had PS 1 activity and was devoid of PS 2 activity. This fraction was four times enriched in P700 when compared with chloroplasts. Fraction 1 had little chl b, a long wavelength absorption maximum in the red, a maximum of low temperature emission fluorescence at 730nm, and a circular dichroism spectrum characteristic of PS 1 enriched fraction.Fraction 2 exhibited a PS 2 activity and no PS 1 activity. It was enriched five times in PS 2 reaction centre and had little chl b and carotenoids. The absorption maximum was at 674 nm and the low temperature fluorescence emission maximum was at 700 nm. Fraction 2 might be useful PS 2 enriched particle because of the great stability of this fraction with regard to photochemical activity and also rapidity and simplicity of its preparation.Fraction 3, which had the fastest migration, was devoid of photochemical activities; It was rich in chl b and had the fluorescence and the circular dichroism spectrum characteristic of an antenna complex.

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F‐695 emission from the purified photosystem II chlorophyll a‐protein complex

Cited by 42 publications

References 20 publications

Organization and Function of Chlorophyll in Membranes of Cyanobacteria during Iron Starvation

Organization and Function of Chlorophyll in Membranes of Cyanobacteria during Iron Starvation

Macromolecular physiology of plastids XIV.Viridis mutants in barley: Genetic, fluoroscopic and ultrastructural characterisation

A single step separation of PS 1, PS 2 and chlorophyll-antenna particles from spinach chloroplasts

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