Ezrin accelerates breast cancer liver metastasis through promoting furin-like convertase-mediated cleavage of Notch1

Chen, Miaojuan; Pan, Yue; Liu, Hanbo; Ning, Fen; Lu, Qin; Duan, Yaoyun; Gan, Xiaowen; Lu, Shenjiao; Hou, Huomei; Zhang, Min; Tian, Ye; Lash, Gendie E.

doi:10.1007/s13402-022-00761-x

Cited by 4 publications

(4 citation statements)

References 47 publications

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“…S1 cleavage of the Notch receptor is necessary for Notch receptor maturation. Furin-like convertase is the protease for the S1 cleavage site [26] . S1 furin-like cleavage in the Golgi is regulated by Botch, and NICD is increased after treatment with furin [ 37 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

“…Fluorogenic furin substrate peptide (Pyr-Arg-Thr-Lys-Arg-AMC; Univ, Shanghai, China) was used to measure furin-like proprotein convertase activity. The experiment was performed as described in previous studies [ 25 , 26 ]. Briefly, Cell lysates were thawed on ice and diluted 2-fold using 5× lysis/reaction buffer, then 20 μL was added to black opaque 96-well plates (Corning, Corning, USA) containing 70 μL of ultrapure water.…”

Section: Methodsmentioning

confidence: 99%

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Fucosyltransferase 9 promotes neuronal differentiation and functional recovery after spinal cord injury by suppressing the activation of Notch signaling

Chen¹,

Zeng²,

Zhang³

et al. 2023

ABBS

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Individuals with spinal cord injury (SCI) suffer from permanent disabilities such as severe motor, sensory and autonomic dysfunction. Neural stem cell transplantation has proven to be a potential strategy to promote regeneration of the spinal cord, since NSCs can produce neurotrophic growth factors and differentiate into mature neurons to reconstruct the injured site. However, it is necessary to optimize the differentiation of NSCs before transplantation to achieve a better regenerative outcome. Inhibition of Notch signaling leads to a transition from NSCs to neurons, while the underlying mechanism remains inadequately understood. Our results demonstrate that overexpression of fucosyltransferase 9 (Fut9), which is upregulated by Wnt4, promotes neuronal differentiation by suppressing the activation of Notch signaling through disruption of furin-like enzyme activity during S1 cleavage. In an in vivo study, Fut9-modified NSCs efficiently differentiates into neurons to promote functional and histological recovery after SCI. Our research provides insight into the mechanisms of Notch signaling and a potential treatment strategy for SCI.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Fucosyltransferase 9 promotes neuronal differentiation and functional recovery after spinal cord injury by suppressing the activation of Notch signaling

Chen¹,

Zeng²,

Zhang³

et al. 2023

ABBS

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“… 24 Notch1 ezrin accelerates breast cancer liver metastasis through promoting furin-like convertase-mediated cleavage of Notch1 mice Chen et al. 25 AGR3 anterior gradient 3 promotes breast cancer metastasis to liver and bone and chemotherapy response human Xu et al. 26 ER estrone, the major postmenopausal estrogen, binds ERa to induce SNAI2, epithelial-to-mesenchymal transition, and ER+ breast cancer metastasis mice Qureshi et al.…”

Section: Factors Contributing To Drug Resistance In Bclmmentioning

confidence: 99%

Mechanisms of drug resistance in breast cancer liver metastases: Dilemmas and opportunities

Yan¹,

Zhao²,

Wei³

et al. 2023

Molecular Therapy - Oncolytics

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“…Much research has indicated that Ezrin overexpression is strongly associated with tumor metastasis and poor patient prognosis, acting as a key player in various cancer types. This has been observed in rhabdomyosarcoma (RMS) [ 215 ], breast cancer [ 220 , 221 ], PDAC [ 202 ], melanoma [ 36 ], ESCC [ 222 ], prostate cancer [ 223 ], and nasopharyngeal carcinoma [ 224 ], among others. Ezrin is abundantly expressed in highly metastatic cells in RMS compared to poorly metastatic cells [ 215 ].…”

Section: Ezrin As a Target For Treating Metastatic Diseasementioning

confidence: 99%

Ez-Metastasizing: The Crucial Roles of Ezrin in Metastasis

Buenaventura,

Merlino,

2023

Cells

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Ezrin is the cytoskeletal organizer and functions in the modulation of membrane–cytoskeleton interaction, maintenance of cell shape and structure, and regulation of cell–cell adhesion and movement, as well as cell survival. Ezrin plays a critical role in regulating tumor metastasis through interaction with other binding proteins. Notably, Ezrin has been reported to interact with immune cells, allowing tumor cells to escape immune attack in metastasis. Here, we review the main functions of Ezrin, the mechanisms through which it acts, its role in tumor metastasis, and its potential as a therapeutic target.

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Ezrin accelerates breast cancer liver metastasis through promoting furin-like convertase-mediated cleavage of Notch1

Cited by 4 publications

References 47 publications

Fucosyltransferase 9 promotes neuronal differentiation and functional recovery after spinal cord injury by suppressing the activation of Notch signaling

Fucosyltransferase 9 promotes neuronal differentiation and functional recovery after spinal cord injury by suppressing the activation of Notch signaling

Mechanisms of drug resistance in breast cancer liver metastases: Dilemmas and opportunities

Ez-Metastasizing: The Crucial Roles of Ezrin in Metastasis

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