EZH2 promotes invasion and tumour glycolysis by regulating STAT3 and FoxO1 signalling in human OSCC cells

Zheng, Min; Cao, Mingxin; Luo, Xinmei; Li, Li; Wang, Ke; Wang, Shasha; Wang, Haofan; Tang, Ya‐Jie; Tang, Yaling; Liang, Xin‐hua

doi:10.1111/jcmm.14579

Cited by 37 publications

(25 citation statements)

References 45 publications

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“…Then using miR-specific primers and universal adaptor PCR primers (Genecopoeia), qRT-PCR was performed with All-in-One™ miRNA qRT-PCR Detection Kit (Genecopoeia) on CFX Connect Real-Time System (Bio-Rad). For mRNA, the reverse transcription was carried out using HiScript II Q RT SuperMix (Vazyme), and qRT-PCR was performed with ChamQ™ SYBR Color qPCR Master Mix (Vazyme) on CFX Connect Real-Time System (Bio-Rad) [21]. Specific primers for qRT-PCR of mRNA were listed in Supplementary Data.…”

Section: Qrt-pcrmentioning

confidence: 99%

RETRACTED ARTICLE: Interplay between cancer cells and M2 macrophages is necessary for miR-550a-3-5p down-regulation-mediated HPV-positive OSCC progression

Cao

Zhang

et al. 2020

J Exp Clin Cancer Res

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Background: Human papillomavirus (HPV)-positive oral squamous cell carcinoma (OSCC) is increasing worldwide with typically higher grade and stage, while better prognosis. microRNAs (miRNAs) has been shown to play a critical role in cancer, however, their role in HPV-positive OSCC progression remains unclear. Methods: miRNA microarray was performed to identify differentially expressed miRNAs. qRT-PCR and FISH were performed to determine the relative expression of miR-550a-3-5p. CCK-8, Flow cytometry, Wound healing, Cell invasion assays and xenograft experiments were conducted to analyze the biological roles of miR-550a-3-5p. Tumor-associated macrophages (TAMs) generation, co-culturing of cancer cells with TAMs, Western blot, Dualluciferase reporter gene assay, Immunohistochemistry and animal studies were performed to explore the mechanisms underlying the functions of miR-550a-3-5p. Results: We identified 19 miRNAs differentially expressed in HPV-positive OSCC specimens and miR-550a-3-5p was down-regulated. The low expression of miR-550a-3-5p correlated with higher tumor size and nodal metastasis of HPV-positive OSCC patients. Then, we found that miR-550a-3-5p suppressed the migration, invasion and EMT of HPV-positive OSCC cells dependent on decreasing M2 macrophages polarization. Moreover, miR-550a-3-5p, down-regulated by E6 oncoprotein, inhibited M2 macrophages polarization by YAP/ CCL2 signaling, which in turn abrogating EMT program in HPV-positive OSCC cells. In addition, in both xenografts and clinical HPV-positive OSCC samples, miR-550a-3-5p levels were inversely associated with YAP, CCL2 expressions and the number of M2 macrophages. Conclusions: E6/miR-550a-3-5p/YAP/CCL2 signaling induces M2 macrophages polarization to enhance EMT and progression, revealing a novel crosstalk between cancer cells and immune cells in HPV-positive OSCC microenvironment.

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Section: Qrt-pcrmentioning

confidence: 99%

RETRACTED ARTICLE: Interplay between cancer cells and M2 macrophages is necessary for miR-550a-3-5p down-regulation-mediated HPV-positive OSCC progression

Cao

Zhang

et al. 2020

J Exp Clin Cancer Res

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View full text Add to dashboard Cite

show abstract

“…Then using miR-speci c primers and universal adaptor PCR primers (Genecopoeia), qRT-PCR was performed with All-in-One TM miRNA qRT-PCR Detection Kit (Genecopoeia) on CFX Connect Real-Time System (Bio-Rad). For mRNA, the reverse transcription was carried out using HiScript II Q RT SuperMix (Vazyme), and qRT-PCR was performed with ChamQ TM SYBR Color qPCR Master Mix (Vazyme) on CFX Connect Real-Time System (Bio-Rad) (21).…”

Section: Qrt-pcrmentioning

confidence: 99%

WITHDRAWN: Interplay between cancer cells and M2 macrophages is necessary for miR-550a-3-5p down-regulation-mediated HPV-positive OSCC progression

Cao

Zhang

et al. 2020

Preprint

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Background: Human papillomavirus (HPV)-positive oral squamous cell carcinoma (OSCC) is increasing worldwide with typically higher grade and stage, while better prognosis. microRNAs (miRNAs) has been shown to play a critical role in cancer, however, their role in HPV-positive OSCC progression remains unclear.Methods: miRNA microarray was performed to identify differentially expressed miRNAs. qRT-PCR and FISH were performed to determine the relative expression of miR-550a-3-5p. CCK-8, Flow cytometry, Wound healing, Cell invasion assays and xenograft experiments were conducted to analyze the biological roles of miR-550a-3-5p. Tumor-associated macrophages (TAMs) generation, co-culturing of cancer cells with TAMs, Western blot, Dual-luciferase reporter gene assay, Immunohistochemistry and animal studies were performed to explore the mechanisms underlying the functions of miR-550a-3-5p.Results: We identified 19 miRNAs differentially expressed in HPV-positive OSCC specimens and miR-550a-3-5p was down-regulated. The low expression of miR-550a-3-5p correlated with higher tumor size and nodal metastasis of HPV-positive OSCC patients. Then, we found that miR-550a-3-5p suppressed the migration, invasion and EMT of HPV-positive OSCC cells dependent on decreasing M2 macrophages polarization. Moreover, miR-550a-3-5p, down-regulated by E6 oncoprotein, inhibited M2 macrophages polarization by YAP/CCL2 signaling, which in turn abrogating EMT program in HPV-positive OSCC cells. In addition, in both xenografts and clinical HPV-positive OSCC samples, miR-550a-3-5p levels were inversely associated with YAP, CCL2 expressions and the number of M2 macrophages.Conclusions: E6/miR-550a-3-5p/YAP/CCL2 signaling induces M2 macrophages polarization to enhance EMT and progression, revealing a novel crosstalk between cancer cells and immune cells in HPV-positive OSCC microenvironment.

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“…Several types of cancer, including OSCC highly depend on glycolysis for ATP generation. Zheng et al (47) found that zeste homolog2 can regulate STAT3 and FoxO1 signaling in human OSCC cells and promote invasion and tumor glycolysis. Another study demonstrated that circMDM2 could promote the proliferation and glycolysis of human OSCC cells by acting as ceRNAs to sponge miR-532-3p (48).…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of the significance of ferroptosis-related genes in the prognosis and immunotherapy of oral squamous cell carcinoma

Yin¹,

Zeng²,

Ai³

et al. 2020

Preprint

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Background: Oral squamous cell carcinoma (OSCC) is a life-threatening disease that emerged as a major international health concern, associated with poor prognosis and the absence of specific biomarkers. Studies have shown that the ferroptosis-related genes (FRGs) can be used as tumor prognostic markers. However, FRGs’ prognostic value in OSCC needs further exploration. Our aim was to construct a novel FRG signature for overall survival (OS) prediction in OSCC patients and explore its role in immunotherapy.Methods: In our study, gene expression profile and clinical data of OSCC patients were collected from a public domain. FRGs were available from the FerrDb database. We performed univariate and multivariate Cox regression analyses to construct a multigene signature. The Kaplan-Meier (K-M) and receiver operating characteristic (ROC) methods were utilized to test the effectiveness of the FRG signature. A differential gene expression analysis was performed by the limma R package, followed by functional enrichment analyses. CIBERSORT was applied to analyze the tumor microenvironment (TME). Finally, the expression of human leukocyte antigen (HLA) and immune checkpoint molecules were analyzed to confirm the sensitivity of immunotherapy.Results: A total of 103 FRGs, expressed in OSCC (FRGs-OSCC), were identified from the two datasets by the Venn analysis. The Cox regression analysis identified 5 FRGs-OSCC that were associated with overall survival (all P < 0.01). The FRGs-OSCC risk model was established to classify patients into high risk and low risk groups. Compared with the low risk group, the survival time of the high-risk group was significantly reduced (P < 0.001). According to the multivariate Cox regression analyses, the risk score acted as an independent predictor for OS (HR > 1, P < 0.001). The accuracy of the FRGs-OSCC risk predictive model was confirmed by ROC curve analysis. The results of the Kyoto Encyclopedia of Genes and Genomes (KEGG) showed significant enrichment of immune-related pathways, and a difference in tumor microenvironment between the two groups. The low risk group had the characteristics of higher expression of HLA and immune checkpoints (IDO1, LAG3, PDCD1 and TIGHT), a lower tumor purity and a higher infiltration of immune cells, indicating a more sensitive response to immunotherapy.Conclusions: The novel FRGs-OSCC risk score system can be used to predict OSCC prognosis. Ferroptosis targeting may be a therapeutic option for OSCC.

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EZH2 promotes invasion and tumour glycolysis by regulating STAT3 and FoxO1 signalling in human OSCC cells

Cited by 37 publications

References 45 publications

RETRACTED ARTICLE: Interplay between cancer cells and M2 macrophages is necessary for miR-550a-3-5p down-regulation-mediated HPV-positive OSCC progression

RETRACTED ARTICLE: Interplay between cancer cells and M2 macrophages is necessary for miR-550a-3-5p down-regulation-mediated HPV-positive OSCC progression

WITHDRAWN: Interplay between cancer cells and M2 macrophages is necessary for miR-550a-3-5p down-regulation-mediated HPV-positive OSCC progression

Comprehensive analysis of the significance of ferroptosis-related genes in the prognosis and immunotherapy of oral squamous cell carcinoma

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