Eyestalk Ablation to Increase Ovarian Maturation in Mud Crabs

Asmat-Ullah, Mohammad; Rozaimi, Rusydi; Fazhan, Hanafiah; Shu-Chien, Alexander Chong; Wang, Youji; Waiho, Khor

doi:10.3791/65039

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Captivity-induced stress in marine invertebrate broodstock, such as handling, overcrowding, diseases, and other inappropriate culture conditions, can interfere with these ovarian maturation-related pathways, which is problematic for their sustainable culture (Gianasi, 2017). In some cultured crustaceans, reproductive dysfunction may be overcome by eyestalk ablation, which stimulates maturation, but it is associated with a high mortality rate (Gianasi, 2017;Jin et al, 2021;Asmat-Ullah et al, 2023;Jia et al, 2023). The use of dsRNA-VIH and reproductionassociated neurohormones has shown promise as a replacement for eyestalk ablation, and is highly effective in enhancing reproductive performance in several crustaceans (Cohen et al, 2021;Kang et al, 2021;Duangprom et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Potent ovarian development as being stimulated by cocktail hormone in the female Scylla olivacea

Saetan,

Duangprom,

Songkoomkrong

et al. 2023

Front. Mar. Sci.

View full text Add to dashboard Cite

The mud crab Scylla olivacea is widely cultured for its economic value, but reproduction issues limit its production. Vitellogenesis-inhibiting hormone (VIH), serotonin (5-HT), and gonadotropin-releasing hormone (GnRH) are important neurohormones that control reproduction in crustaceans. Mimicking crab hormone stimulation during reproduction has scarcely been reported. Comparison of the single hormone and multiple hormone approaches to crab hormonal control in S. olivacea is limited. In situ hybridization showed that injection of dsRNA-VIH could abolish its gene expression in neuronal clusters of female S. olivacea eyestalks, potentially reducing its inhibitory effects on ovarian maturation. This was confirmed by assessing the ovarian gonadosomatic index (GSI), hemolymph vitellogenin (Vg), an indicator of vitellogenesis, and gonad histology using dsRNA-VIH and 5-HT/GnRH combinations. Based on our findings, we demonstrated that administration of dsRNA-VIH significantly increased the gonadosomatic index (GSI) on days 14 and 28 post-treatment. The combination cocktail, however, consisting of 5-HT + GnRH + dsRNA-VIH on days 14 and 28, and GnRH + dsRNA-VIH on day 28, was the most efficacious in increasing GSI and enhancing crab ovarian maturation. Upregulation of hemolymph Vg levels was observed solely on the 28th day following treatment with dsRNA-VIH, 5-HT + GnRH + dsRNA-VIH, and GnRH + dsRNA-VIH. Differential gene expression analysis using quantitative RNA-sequencing of the neural tissues (brain and ventral nerve cord), revealed a significant upregulation of certain receptors (5-HTR, GnRHR, LHR, and FSHR), neuropeptides (sNPF, NPF1, NPF2, SIFamide, AKH/Crz, CHH, and RPCH), downstream reproductive-related genes (FAMeT, ESULT, progesterone-like protein), and prostanoid-related genes (phospholipase A and C, COX, Thromboxane A synthase, prostaglandin D, E, and F synthases) following treatment, particularly dsRNA-VIH + GnRH and/or 5-HT-injected individuals. Upregulation of prostaglandin E synthase and estrogen sulfotransferase genes was confirmed by real-time PCR. Since the construction and propagation of dsRNA-VIH is costly, its lower dose application supplemented with synthetic GnRH and/or 5-HT may be an alternative approach to ensure that female S. olivacea attain sufficient reproductive fecundity in aquaculture. Furthermore, we propose that the administration of multiple hormones in crabs may better emulate the physiological conditions of crustaceans in their natural habitat.

show abstract