Eye development

Baker, Nicholas E.; Li, Ke; Quiquand, Manon; Ruggiero, Robert P.; Wang, Lan-Hsin

doi:10.1016/j.ymeth.2014.04.007

Cited by 35 publications

(30 citation statements)

References 80 publications

(76 reference statements)

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“…Immunochemistry and scanning electron microscopy were performed as described (Firth et al, 2006; Baker et al, 2014). Flp-on expression clones were generated by crossing UAS -lines to hs-FLP 122 ; Act5C>CD2> GAL4 UAS-GFP.…”

Section: Methodsmentioning

confidence: 99%

Salvador-Warts-Hippo Pathway in a Developmental Checkpoint Monitoring Helix-Loop-Helix Proteins

Wang

Baker

2015

Developmental Cell

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The E-proteins and Id-proteins are, respectively, the positive and negative heterodimer partners for the basic-helix-loop-helix protein family, and as such contribute to a remarkably large number of cell fate decisions. E-proteins and Id-proteins also function to inhibit or promote cell proliferation and cancer. Using a genetic modifier screen in Drosophila, we show that the Id-protein Extramacrochaetae enables growth by suppressing activation of the Salvador-Warts-Hippo pathway of tumor suppressors, activation that requires transcriptional activation of the expanded gene by the E-protein Daughterless. Daughterless protein binds to an intronic enhancer in the expanded gene, both activating the SWH pathway independently of the transmembrane protein Crumbs, and bypassing the negative feedback regulation that targets the same expanded enhancer. Thus the Salvador-Warts-Hippo pathway has a cell-autonomous function to prevent inappropriate differentiation due to transcription factor imbalance, and monitors the intrinsic developmental status of progenitor cells, distinct from any responses to cell-cell interactions.

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Section: Methodsmentioning

confidence: 99%

Salvador-Warts-Hippo Pathway in a Developmental Checkpoint Monitoring Helix-Loop-Helix Proteins

Wang

Baker

2015

Developmental Cell

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“…Antibody staining was performed as previously described (Baker et al, 2014). The following primary antibodies were used: mouse anti-βGal (1:100, DSHB 40-1a), rabbit anti-βGal (Cappell), rat anti-ElaV(1:50, DSHB 7E8A10), mouse anti-Da (1:200)(Cronmiller and Cummings, 1993), rabbit anti-Emc (1:8000, a gift from Y. N. Jan), rabbit anti-Ato (1:50000)(Jarman et al, 1994), rat anti-GFP(1:1000, Nacalai Tesque GF090R), mouse anti-Dl (mAb202).…”

Section: Methodsmentioning

confidence: 99%

The Notch pathway regulates the Second Mitotic Wave cell cycle independently of bHLH proteins

Bhattacharya

Quiquand

et al. 2017

Developmental Biology

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Notch regulates both neurogenesis and cell cycle activity to coordinate precursor cell generation in the differentiating Drosophila eye. Mosaic analysis with mitotic clones mutant for Notch components was used to identify the pathway of Notch signaling that regulates the cell cycle in the Second Mitotic Wave. Although S phase entry depends on Notch signaling and on the transcription factor Su(H), the transcriptional co-activator Mam and the bHLH repressor genes of the E(spl)-Complex were not essential, although these are Su(H) coactivators and targets during the regulation of neurogenesis. The Second Mitotic Wave showed little dependence on ubiquitin ligases neuralized or mindbomb, and although the ligand Delta is required non-autonomously, partial cell cycle activity occurred in the absence of known Notch ligands. We found that myc was not essential for the Second Mitotic Wave. The Second Mitotic Wave did not require the HLH protein Extra macrochaetae, and the bHLH protein Daughterless was required only cell-nonautonomously. Similar cell cycle phenotypes for Daughterless and Atonal were consistent with requirement for neuronal differentiation to stimulate Delta expression, affecting Notch activity in the Second Mitotic Wave indirectly. Therefore Notch signaling acts to regulate the Second Mitotic Wave without activating bHLH gene targets.

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“…Immunochemistry procedures have been described[44]. Orientation of cell divisions was measured as described previously[8].…”

Section: Methodsmentioning

confidence: 99%

Local Cell Death Changes the Orientation of Cell Division in the Developing Drosophila Wing Imaginal Disc Without Using Fat or Dachsous as Orienting Signals

2016

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Drosophila imaginal disc cells exhibit preferred cell division orientations according to location within the disc. These orientations are altered if cell death occurs within the epithelium, such as is caused by cell competition or by genotypes affecting cell survival. Both normal cell division orientations, and their orientations after cell death, depend on the Fat-Dachsous pathway of planar cell polarity (PCP). The hypothesis that cell death initiates a planar polarity signal was investigated. When clones homozygous for the pineapple eye (pie) mutation were made to initiate cell death, neither Dachsous nor Fat was required in pie cells for the re-orientation of nearby cells, indicating a distinct signal for this PCP pathway. Dpp and Wg were also not needed for pie clones to re-orient cell division. Cell shapes were evaluated in wild type and mosaic wing discs to assess mechanical consequences of cell loss. Although proximal wing disc cells and cells close to the dorso-ventral boundary were elongated in their preferred cell division axes in wild type discs, cell shapes in much of the wing pouch were symmetrical on average and did not predict their preferred division axis. Cells in pie mutant clones were slightly larger than their normal counterparts, consistent with mechanical stretching following cell loss, but no bias in cell shape was detected in the surrounding cells. These findings indicate that an unidentified signal influences PCP-dependent cell division orientation in imaginal discs.

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Eye development

Cited by 35 publications

References 80 publications

Salvador-Warts-Hippo Pathway in a Developmental Checkpoint Monitoring Helix-Loop-Helix Proteins

Salvador-Warts-Hippo Pathway in a Developmental Checkpoint Monitoring Helix-Loop-Helix Proteins

The Notch pathway regulates the Second Mitotic Wave cell cycle independently of bHLH proteins

Local Cell Death Changes the Orientation of Cell Division in the Developing Drosophila Wing Imaginal Disc Without Using Fat or Dachsous as Orienting Signals

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